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The Effect of Dynamic High Pressure Microfluidizationon the Activity of Papain
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Wei LIU, Ye-Jun ZHONG, Cheng-Mei LIU, Ming-Yong XIE, Bin GUAN, Man YIN, Qian WANG, Ting-Ting CHEN
Chinese Journal of High Pressure Physics | 2010, 24(2) : 129 - 135
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Chinese Journal of High Pressure Physics | 2010, 24(2): 129-135
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The Effect of Dynamic High Pressure Microfluidizationon the Activity of Papain
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Wei LIU, Ye-Jun ZHONG, Cheng-Mei LIU, Ming-Yong XIE, Bin GUAN, Man YIN, Qian WANG, Ting-Ting CHEN
Affiliations
  • State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

    • LIU Wei (1972—), male, doctoral candidate, associate professor, major in food engineering technology. E-mail:

About Author:

LIU Wei (1972—), male, doctoral candidate, associate professor, major in food engineering technology. E-mail:

Published: 2010-04-15
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The effect of dynamic high pressure microfluidization (DHPM) on the activity of papain had been investigated, and the molecular conformation changes of papain had been detected by fluorescence spectra. The results showed that after DHPM (120~180 MPa) treatment, the enzyme activity decreased in different degrees. The papain activity reduced to 90.04% after 180 MPa treatment. With the increasing of treatment pressure, the fluorescence emission peaks of papain, Tyr and Trp residues gradually red shifted from 334, 285 and 277.5 nm for untreated sample to 335.5, 285.5 and 278.5 nm for 140 MPa treated sample, and then returned to 334, 285 and 278 nm for 180 MPa treated sample, respectively. After 24 h placing at 0~4 ℃, papain activity further decreased and the fluorescence intensity of papain and Tyr residue showed oscillate (down, up and down), which indicated that DHPM treatment (120~180 MPa) changed the molecular conformation of papain visibly with low stability of the new formed conformation.

dynamic high pressure microfluidization (DHPM)  /  fluorescence spectra  /  papain  /  molecular conformation
刘伟, 钟业俊, 刘成梅, 谢明勇, 官斌, 尹曼, 王倩, 陈婷婷. 动态高压微射流技术对木瓜蛋白酶活性的影响. 高压物理学报, 2010 , 24 (2) : 129 -135 .
Wei LIU, Ye-Jun ZHONG, Cheng-Mei LIU, Ming-Yong XIE, Bin GUAN, Man YIN, Qian WANG, Ting-Ting CHEN. The Effect of Dynamic High Pressure Microfluidizationon the Activity of Papain[J]. Chinese Journal of High Pressure Physics, 2010 , 24 (2) : 129 -135 .
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Funding
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  • National High Technology Research and Development Program (863 Program)(2007AA100403)
  • Research Program of Sate Key Laboratory of Food Science and Technology, Nanchang University(SKLF-MB-200808)
References
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Year 2010 volume 24 Issue 2
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Article Info
  • Receive Date：2009-02-23
  • Online Date：2026-10-30
  • Published：2010-04-15
Article Data
Affiliations
History
  • Received：2009-02-23
  • Revised：2009-04-07
Funding
National High Technology Research and Development Program (863 Program)(2007AA100403)
Research Program of Sate Key Laboratory of Food Science and Technology, Nanchang University(SKLF-MB-200808)
Affiliations
    State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China

Corresponding:

LIU Cheng-Mei (1963—), male, doctor, professor, doctoral supervisor, major in food science. E-mail:
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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