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Construction and Analysis of SSH-cDNA Library Induced by High Pressure in Rice Seeds during Germination
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Xun-Cheng LIU1, 2, Mei ZHANG1, Jun DUAN1
Chinese Journal of High Pressure Physics | 2008, 22(4) : 370 - 376
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Chinese Journal of High Pressure Physics | 2008, 22(4): 370-376
Construction and Analysis of SSH-cDNA Library Induced by High Pressure in Rice Seeds during Germination
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Xun-Cheng LIU1, 2, Mei ZHANG1, Jun DUAN1
Affiliations
  • 1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China
  • 2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China
Published: 2008-12-05
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In order to study the effect of expression pattern of high hydrostatic pressure on high plant, a SSH-cDNA library was constructed with rice germinating seeds in this paper. Suppression subtractive hybridization (SSH) in combination with mirror orientation selection (MOS) was performed using the mRNA from rice germinating seeds treated by 75 MPa high hydrostatic pressure as tester and the mRNA from control (seeds untreated by high hydrostatic pressure) as the driver. 580 recombinant clones were picked through blue/white screening. PCR detection of the recombinant clones showed that the size of inserts mainly ranged from 200 bp to 1 000 bp, and the average size of these inserts were about 575 bp. Among the 580 recombinant clones, 542 clones (93.3%) were identified to be effective single band ones. After sequencing some of the recombinant clones, it was found that the genes induced by high hydrostatic pressure were mainly involved in metabolism, stress response and transcriptional regulation. The research paved the way for studying the gene expression patterns of rice seeds responding to high hydrostatic pressure.

high hydrostatic pressure  /  suppression subtractive hybridization (SSH)  /  rice
刘勋成, 张美, 段俊. 水稻种子萌发期高压诱导的SSH-cDNA文库构建与分析. 高压物理学报, 2008 , 22 (4) : 370 -376 .
Xun-Cheng LIU, Mei ZHANG, Jun DUAN. Construction and Analysis of SSH-cDNA Library Induced by High Pressure in Rice Seeds during Germination[J]. Chinese Journal of High Pressure Physics, 2008 , 22 (4) : 370 -376 .
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Year 2008 volume 22 Issue 4
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Article Info
  • Receive Date：2007-11-28
  • Online Date：2026-10-30
  • Published：2008-12-05
Article Data
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History
  • Received：2007-11-28
  • Revised：2008-03-28
Funding
Affiliations
    1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China
    2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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