The mitochondrial genome (mitogenome) of hybrid grouper Epinephelus moara (♀)×Epinephelus tukula (♂), a new hybrid progeny, can provide valuable information for analyzing phylogeny and molecular evolution. In this study, the mitogenome was analyzed using PCR amplification and sequenced, then the phylogenetic relationship of E. moara (♀)×E. tukula (♂) and 35 other species were constructed using Maximum Likelihood and Neighbor-Joining methods with the nucleotide sequences of 13 conserved protein-coding genes (PCGs). The complete mitogenome of E. moara (♀)×E. tukula (♂) was 16 695 bp in length, which contained 13 PCGs, 2 rRNA genes, 22 tRNA genes, a replication origin and a control region. The composition and order of these genes were consistent with most other vertebrates. Of the 13 PCGs, 12 PCGs were encoded on the heavy strand, and ND6 was encoded on the light strand. The mitogenome of the E. moara (♀)×E. tukula (♂) had a higher AT nucleotide content, a positive AT-skew and a negative GC-skew. All protein initiation codons were ATG, except for COX and ND4 (GTG), ATP6 (CTG), and ND3 (ATA). ND2, COXII, ND3, ND4 and Cytb had T as the terminating codon, COXIII’s termination codon was TA, and the remaining PCGs of that were TAA. All tRNA genes, except for the lacking DHU-arm of tRNA^{Ser (AGN)}, were predicted to form a typical cloverleaf secondary structure. In addition, sequence similarity analysis (99% identity) and phylogenetic analysis (100% bootstrap value) indicated that the mitochondrial genome was maternally inherited. This study provides mitogenome data for studying genetic, phylogenetic relationships and breeding of grouper.

This study provides new insights for the hatchery released Chinese shrimp (Fenneropenaeus chinensis), including proportion, dynamic migration route, after they were released into nature for stock enhancement using a new strategy quite different than ever. Chinese shrimp were sampled at 22 survey stations during two investigation voyages acrossing 74 survey stations in the Bohai Sea from July 16 to August 9 in 2015. Among 289 sampled individuals during the second voyage, totally 155 shrimps were identified as hatchery shrimp released into the Laizhou Bay at mid-May in 2015 based on finger-print of eight SSR (simple sequence repeats) markers, and the proportion of hatchery released shrimp in recapture samples were from 41.30%–85.71% in each station with an average value 53.63%, which verified a previous view point that up to 90% of autumn season Chinese shrimp landing in the Bohai Sea were composed of hatchery released. Meanwhile, the dynamic migration route of hatchery released shrimp revealed that part of released shrimp migrated heading northwest along the west coast of the Bohai Sea up to the Bohai Bay but just remained at the Laizhou Bay until over-wintering migration at mid-October when they initiate over-wintering migration. Present unnatural spring season shrimp fishing model cut the throat of spawner shrimp chance to swim back to their respective spawning plants at each spring, it still no chance to clarify whether the hatchery released shrimp could replenish to the reproduce population and complete a whole life cycle as same as their natural relatives.

Medusae in 116 samples collected from the coastal waters of the northern Beibu Gulf during four seasonal oceanographic cruises from October 2017 to August 2018 were analyzed. In total, 34 species were identified. Two of the species are new to science, Proboscidactyla pentacanalis Xu, Chen & Yang sp. nov. and Helgicirrha apapillata Xu, Chen & Wang sp. nov., and three species are newly recorded in the Beibu Gulf, i.e., Hydractinia vacuolata Xu & Huang, 2006, Proboscidactyla flavicirrata Brandnt, 1834 and Phialella macrogona Xu, Huang & Wang, 1985. Collections of the species were deposited at the Third Institute of Oceanography, Ministry of Natural Resources.

The scuticociliatid ciliates Ancistrum haliotis and A. crassum are parasites that may cause high mortality in the cultured abalone Haliotis spp. and the bivalve Ruditapes philippinarum. Traditional identification with silver staining methods is hampered by their morphological similarities to closely related species and the complicated procedures of morphological analysis. We designed two SSU rRNA-targeted oligonucleotide probes labeled with a fluorochrome, and optimized the fluorescence in situ hybridization (FISH) protocols for identification of A. halioti and A. crassum, respectively. The assays resulted in a clear identification by strong fluorescence signals from the oligonucleotide probes. The method can be used for quick and accurate quantitative analysis of A. haliotis and A. crassum infections on host molluscs.

Parent and alkylated polycyclic aromatic hydrocarbons (alkyl-PAHs), which are a class of important toxic components of crude oil especially in the marine environment, exhibit adverse effects on aquatic life and potentially pose a human health risk. However, the lack of chronic toxicity data is one of the hindrances for alkyl-PAHs when assessing their ecological risks. In this study, predicted no-effect concentrations (PNECs) in seawater and marine sediment for ten parent- and alkyl-PAHs were derived by applying species sensitivity distributions (SSDs) and quantitative structure−activity relationships (QSARs). The local area, Dalian Bay, where an oil-spilled accident happened in 2010, was chosen as a case site to assess ecological risks for ten PAHs in surface seawaters and marine sediments. Their PNECs in seawater and sediment for protecting aquatic organisms in marine ecosystems were calculated and recommended in the range of 0.012−2.79 μg/L and 48.2−1337 ng/g (dry weight), respectively. Overall, the derived PNECs for the studied PAHs in seawater and marine sediment were comparable to those obtained by classical methods. Risk quotient results indicate low ecological risks to ecosystems for ten parent- and alkyl-PAHs in surface seawaters and surface sediments from the Dalian Bay. These findings provide a first insight into the PNECs and ecological risks of alkyl-PAHs, emphasizing the role of the computational toxicology in ecological risk assessments. The use of QSARs has been identified as a valuable tool for preliminarily assessing ecological risks of emerging pollutants, being more predictable of real exposure scenarios for risk assessment purposes.

Oceanic turbulence measurements made by an acoustic Doppler velocimeter (ADV) suffer from noise that potentially affects the estimates of turbulence statistics. This study examines the abilities of Kalman filtering and autoregressive moving average models to eliminate noise in ADV velocity datasets of laboratory experiments and offshore observations. Results show that the two methods have similar performance in ADV de-noising, and both effectively reduce noise in ADV velocities, even in cases of high noise. They eliminate the noise floor at high frequencies of the velocity spectra, leading to a longer range that effectively fits the Kolmogorov −5/3 slope at mid-range frequencies. After de-noising adopting the two methods, the values of the mean velocity are almost unchanged, while the root-mean-square horizontal velocities and thus turbulent kinetic energy decrease appreciably in these experiments. The Reynolds stress is also affected by high noise levels, and de-noising thus reduces uncertainties in estimating the Reynolds stress.

Imaging altimeter (IALT) is a new type of radar altimeter system. In contrast to the conventional nadir-looking altimeters, such as HY-2A altimeter, Jason-1/2, and TOPEX/Poseidon, IALT observes the earth surface at low incident angles (2.5°–8°), so its swath is much wider and its spatial resolution is much higher than the previous altimeters. This paper presents a wind speed inversion method for the recently launched IALT onboard Tiangong-2 space station. Since the current calibration results of IALT do not agree well with the well-known wind geophysical model function at low incidence angles, a neural network is used to retrieve the ocean surface wind speed in this study. The wind speed inversion accuracy is evaluated by comparing with the ECMWF reanalysis wind speed, buoy wind speed, and in-situ ship measurements. The results show that the retrieved wind speed bias is about –0.21 m/s, and the root-mean-square (RMS) error is about 1.85 m/s. The wind speed accuracy of IALT meets the performance requirement.

The morphological evolution of the sand barrier in the Anhaiao coastal zone of Pingtan from 1996 to 2018 was studied. Tidal correction was used to refine the location of the coastline. A standard deviation ellipse method was applied to further analyze the movement of the barrier head with the axis and rotation angle. A natural neighbor interpolation (NNI) method was carried out to calculate the terrain of the intertidal area, and the erosion and deposition characteristics were illustrated based on the terrain. The results showed that the northern part of the sand barrier facing the lagoon area was deposited over the whole studied period, while erosion has always occurred in the southern part of the sand barrier facing the open sea. The erosion and deposition were slightly different on both sides of the barrier head due to hydrodynamic turbulence. The middle sand barrier moved 102.60 m away from its original location in 1996, and the end of the barrier moved 65.45 m. The head of the sand barrier continued moving 379 m to the northwest. Consequently, the preliminary morphological evolution of the sand barrier corresponding to the distance and direction of movement was detected.

This study investigates the submesoscale fronts and their dynamic effects on the mean flow due to frontal instabilities in the wind-driven summer offshore jet of the western South China Sea (WSCS), using satellite observations, a 500 m-resolution numerical simulation, and diagnostic analysis. Both satellite measurements and simulation results show that the submesoscale fronts occupying a typical lateral scale of O(~10) km are characterized with one order of Rossby (Ro) and Richardson (Ri) numbers in the WSCS. This result implies that both geostrophic and ageostrophic motions feature in these submesoscale fronts. The diagnostic results indicate that a net cross-frontal Ekman transport driven by down-front wind forcing effectively advects cold water over warm water. By this way, the weakened local stratification and strong lateral buoyancy gradients are conducive to a negative Ertel potential vorticity (PV) and triggering frontal symmetric instability (SI) at the submesoscale density front. The cross-front ageostrophic secondary circulation caused by frontal instabilities is found to drive an enhanced vertical velocity reaching O(100) m/d. Additionally, the estimate of the down-front wind forcing the Ekman buoyancy flux (EBF) is found to be scaled with the geostrophic shear production (GSP) and buoyancy flux (BFLUX), which are the two primary energy sources for submesoscale turbulence. The large values of GSP and BFLUX at the fronts suggest an efficient downscale energy transfer from larger-scale geostrophic flows to the submesoscale turbulence owing to down-front wind forcing and frontal instabilities. In this content, submesoscale fronts and their instabilities substantially enhance the local vertical exchanges and geostrophic energy cascade towards smaller-scale. These active submesoscale processes associated density fronts and filaments likely provide new physical interpretations for the filamentary high chlorophyll concentration and frontal downscale energy transfer in the WSCS.

An inverse reduced-gravity model is used to simulate the deep South China Sea (SCS) circulation. A set of experiments are conducted using this model to study the influence of the Luzon overflow through the two inlets on the deep circulation in the northern SCS. Model results suggest that the relative contribution of these inlets largely depends on the magnitude of the input transport of the overflow, but the northern inlet is more efficient than the southern inlet in driving the deep circulation in the northern SCS. When all of the Luzon overflow occurs through the northern inlet the deep circulation in the northern SCS is enhanced. Conversely, when all of the Luzon overflow occurs through the southern inlet the circulation in the northern SCS is weakened. A Lagrangian trajectory model is also developed and applied to these cases. The Lagrangian results indicate that the location of the Luzon overflow likely has impacts upon the sediment transport into the northern SCS.