Dietary studies of polyps and ephyrae are important to understand the formation and magnitude of jellyfish blooms and provide important insights into the marine food web. However, the diet of polyps and ephyrae in situ is largely unknown. Here, prey species of the polyps and ephyrae of the moon jellyfish Aurelia coerulea in situ were identified using high-throughput DNA sequencing techniques. The results show that A. coerulea polyps and ephyrae consume a variety of prey items. The polyps consume both planktonic and benthic prey, including hydromedusae, copepods, ciliates, polychaetes, stauromedusae, and phytoplankton. A. coerulea ephyrae mainly feed on copepods and hydromedusae. Gelatinous zooplankton, including Rathkea octopunctata and Sarsia tubulosa, were frequently found as part of the diet of A. coerulea polyps and ephyrae. The utilization of high-throughput sequencing technique is a useful tool for studying the diet of polyps and ephyrae in the field, complementing the traditional techniques towards a better understanding of the complex role of gelatinous animals in marine ecosystems.

DNA polymerase δ (Polδ) plays a crucial and versatile role in DNA replication and DNA repair processes. Vent shrimp Rimicaris exoculata is the primary megafaunal community living in hydrothermal vents. In this study, the Polδ from shrimp Rimicaris exoculata was cloned, expressed and characterized. The results showed that the Polδ catalytic subunit (POLD1), 852 amino acids in length, shared high homology with crayfish Procambarus clarkii and shrimp Oratosquilla oratoria. The recombinant POLD1 expressed in Escherichia coli showed that the enzyme was active in a range of 20°C to 40°C with an optimum temperature at 25°C and in a wide range of pH with an optimum at pH 6.0. The activities of POLD1 were significantly enhanced in the presence of Triton-X 100, Tween 20 and Mn²⁺. The K_m (dNTP) value of POLD1 was 4.7 μmol/L. The present study would be helpful to reveal the characterization of Polδ of deep-sea vent animals.

This paper applies the narrow band Internet of things communication technology to develop a wireless network equipment and communication system, which can quickly set up a network with a radius of 100 km on water surface. A disposable micro buoy based on narrow-band Internet of things and Beidou positioning function is also developed and used to collect surface hydrodynamic data online. In addition, a web-based public service platform is designed for the analysis and visualization of the data collected by buoys. Combined with the satellite remote sensing data, the study carries a series of marine experiments and studies such as sediment deposition tracking and garbage floating tracking.

In order to assess the effect of enhancement release of chum salmon (Oncorhynchus keta), otolith strontium (Sr) marking method was used to tag chum salmon released in Tangwang River in 2016. The homing chum salmon were detected and the samples were collected in Tangwang River, Ussuri River and Suifen River in the autumn of 2018. The samples were analyzed by examining Sr and calcium (Ca) fingerprints in the otolith using electron probe microanalysis. The results suggested that two samples collected in Tangwang River had the marking ring near the core of otolith where the Sr concentration and Sr/Ca ratio were significantly higher than comparative samples. Proving that the two fish belonged to the released population in Tangwang River in 2016. This article indicated the success of the enhancement release of chum salmon from the Tangwang River for the first time and also confirmed the validity of Sr marking in enhancement release of fishes.

Using the hydrographic data obtained during two nearly simultaneous surveys in June 2015, we carried out semi-diagnostic calculations with the help of a finite element model and a modified inverse method, to study the circulation in the northern South China Sea (NSCS) during the early summer of 2015. A number of new circulation features were found. (1) In most of the observation region, a large, basin-scale anticyclonic gyre appeared south of the 50-m isobath, which contained anticyclonic eddies. One anticyclonic eddy existed from the sea surface to 50-m depth, whose center showed no tilt, while the center of another eddy tilted eastward from the sea surface to 500-m depth. In the eastern part of the observation region, which is west of the Dongsha Islands, there was a sub-basin-scale cyclonic gyre containing a cyclonic eddy whose center tilted southward from the sea surface to 200-m depth. (2) There was a cross-continental slope current (CCSC) in the area southwest of the Dongsha Islands. Its volume transport was about 2.0×10⁶ m³/s. (3) From the estimated order of magnitude of the stream function equation, the joint effect term of the baroclinity and relief (JEBAR) and β-effect term are two important dynamic mechanisms affecting the variation of the circulation in the NSCS. (4) The JEBAR, as a transport-generating term, resulted in the dynamic mechanism determining the pattern of the depth-averaged flow across the contours of potential vorticity fH^–1. Furthermore, we show that the negative values of the JEBAR were the most dominant dynamic mechanism, causing the CCSC southwest of the Dongsha Islands to deflect from the isobaths and veer toward the deep water. The CCSC around the Dongsha Islands was located further southwest during the early summer of 2015 than during the fall of 2005 (revealed by a published study), which suggests that the location of the CCSC around the Dongsha Islands may vary with season.

Satellite-tracked Lagrangian drifters are used to investigate the transport pathways of near-surface water around the Luzon Strait. Particular attention is paid to the intrusion of Pacific water into the South China Sea (SCS). Results from drifter observations suggest that except for the Kuroshio water, other Pacific water that carried by zonal jets, Ekman currents or eddies, can also intrude into the SCS. Motivated by this origin problem of the intrusion water, numerous simulated trajectories are constructed by altimeter-based velocities. Quantitative estimates from simulated trajectories suggest that the contribution of other Pacific water to the total intrusion flux in the Luzon Strait is approximately 13% on average, much smaller than that of Kuroshio water. Even so, over multiple years and many individual intrusion events, the contribution from other Pacific water is quite considerable. The interannual signal in the intrusion flux of these Pacific water might be closely related to variations in a wintertime westward current and eddy activities east of the Luzon Strait. We also found that Ekman drift could significantly contribute to the intrusion of Pacific water and could affect the spreading of intrusion water in the SCS. A case study of an eddy-related intrusion is presented to show the detailed processes of the intrusion of Pacific water and the eddy-Kuroshio interaction.

At the end of May 2008, a massive bloom of macroalgae occurred in the western Yellow Sea off China and lasted for nearly two months, and annual blooms have occurred since then on. During bloom period, the surface-drifting macroalgae have showed an interesting pattern dominated by a banded structure, and the distance between neighboring bands ranged from hundreds of meters to about 6 km with a peak at 1–1.5 km, which is an order of higher than the scale of Langmuir circulation of 50–100 m. In order to explain this new phenomenon, ocean current data obtained from a Doppler current profiler off Qingdao was used to implement stability analysis. By numerically solving the resulting differential Orr-Sommerfeld equation, the secondary circulation induced from the instability of the Emkan current was found to fit well with the observed spatial scale of the surface-drifting macroalgae’s banded structure. As the wind driven Emkan current exist universally in the global ocean, it is reasonable to conclude that the banded structure with kilometers distance between adjoining bands is ubiquitous. We found a new circulation in the upper ocean which is important for exchange of energy, materials and gas between the upper ocean and subsurface layer.

Oceanic geostrophic turbulence theory predicts significant inverse kinetic energy (KE) cascades at scales larger than the energy injection wavelength. However, the characteristics of the mesoscale variabilities associated with the inverse KE cascade in the real oceans have not been clear enough up to now. To further examine this problem, we analyzed the spectral characteristics of the oceanic mesoscale motions over the scales of inverse KE cascades based on high-resolution gridded altimeter data. The applicability of the quasigeostrophic (QG) turbulence theory and the surface quasigeostrophic (SQG) turbulence theory in real oceans is further explored. The results show that the sea surface height (SSH) spectral slope is linearly related to the eddy-kinetic-energy (EKE) level with a high correlation coefficient value of 0.67. The findings also suggest that the QG turbulence theory is an appropriate dynamic framework at the edge of high-EKE regions and that the SQG theory is more suitable in tropical regions and low-EKE regions at mid-high latitudes. New anisotropic characteristics of the inverse KE cascade are also provided. These results indicate that the along-track spectrum used by previous studies cannot reveal the dynamics of the mesoscale variabilities well.

Wave radiation stress is the main driving force of wave-induced near-shore currents. It is directly related to the hydrodynamic characteristics of near-shore current whether the calculation of wave radiation stress is accurate or not. Irregular waves are more capable of reacting wave motion in the ocean compared to regular waves. Therefore, the calculation of the radiation stress under irregular waves will be more able to reflect the wave driving force in the actual near-shore current. Exact solution and approximate solution of the irregular wave radiation stress are derived in this paper and the two kinds of calculation methods are compared. On the basis of this, the experimental results are used to further verify the calculation of wave energy in the approximate calculation method. The results show that the approximate calculation method of irregular wave radiation stress has a good accuracy under the condition of narrow-band spectrum, which can save a lot of computing time, and thus improve the efficiency of calculation. However, the exact calculation method can more accurately reflect the fluctuation of radiation stress at each moment and each location.

A deep-learning-based method, called ConvLSTMP3, is developed to predict the sea surface heights (SSHs). ConvLSTMP3 is data-driven by treating the SSH prediction problem as the one of extracting the spatial-temporal features of SSHs, in which the spatial features are “learned” by convolutional operations while the temporal features are tracked by long short term memory (LSTM). Trained by a reanalysis dataset of the South China Sea (SCS), ConvLSTMP3 is applied to the SSH prediction in a region of the SCS east off Vietnam coast featured with eddied and offshore currents in summer. Experimental results show that ConvLSTMP3 achieves a good prediction skill with a mean RMSE of 0.057 m and accuracy of 93.4% averaged over a 15-d prediction period. In particular, ConvLSTMP3 shows a better performance in predicting the temporal evolution of mesoscale eddies in the region than a full-dynamics ocean model. Given the much less computation in the prediction required by ConvLSTMP3, our study suggests that the deep learning technique is very useful and effective in the SSH prediction, and could be an alternative way in the operational prediction for ocean environments in the future.