While prokaryotes play key roles in nutrient cycling and energy flow during Phaeocystis globosa blooms, the information on the spatial and diel temporal distribution of the prokaryotic community during Phaeocystis blooms remains scarce. In January 2019, we used high-throughput sequencing of the 16S rRNA gene to explore the spatial and diel variations of particle-attached (PA) and free-living (FL) prokaryotic communities during the blooming phase of P. globosa in Beibu Gulf, Guangxi, China. The results suggested a significant spatial variation pattern in the horizontal distribution of prokaryotic communities, while there was no significant difference in the vertical direction. Both spatial distance and environmental variables shaped the horizontal distribution of the prokaryotic community structure, while environmental variables, particularly the abundance of P. globosa colony and Chl a, showed more significant influence and were closely related to the structure and variation of the prokaryotic community. Strong vertical mixing of the water column disrupted the vertical structure heterogeneity of the prokaryotic community in winter. There were significant differences in the diel samples of PA prokaryotic communities, but not in the FL prokaryotic communities. Nitrate, ammonium and the abundance of P. globosa colony were the key environmental variables impacting the diel variations of prokaryotic communities over the sampling period. The present study provided valuable information to depict the spatial-temporal variations of the microbial community and its association with environmental parameters during P. globosa bloom in the tropical gulf.

The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal (HPG) axis in vertebrates. Turbot (Scophthalmus maximus) has become an economically important marine fish in Europe and North China. Previous research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm production. However, the morphology and histology of the organs in the HPG axis have not been studied. In this study, we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal development. The results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts: the telencephalon, diencephalon, cerebellum, hypothalamus, pituitary gland, myelencephalon, and olfactory bulbs. The telencephalon was well-developed and contained five distinct lobes, with the contiguous diencephalon at the caudal portion. The torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface, and the rostral corpus cerebellum was located in the dorsal portion. The actual total brain volume in mature males was significantly greater (p<0.05) than that of females with gonadal development. Notably, the pituitary volume in male turbot significantly increased (p<0.05) from immature to mature stage, but this difference did not occur in females. The data together illustrate a distinct sex difference in the turbot brain during gonadal development, providing insight into their HPG axes.

High-resolution multi-beam/single-beam bathymetric data and seismic profiling data from the latest surveys are used to map and interpret the detailed seafloor geomorphology of the western region of the North Yellow Sea (NYS), China. The mapping area covers 156 410 km², and incorporates a flat shelf plain, subaqueous accumulation shoals, tidal scouring troughs, and tidal sand ridge groups. Offshore areas with water depths less than 50 m in the western region of the NYS are mainly covered by thick, loose sediments, forming wide spread accumulation geomorphological features; these include the Liaodong Peninsula subaqueous accumulation system containing shoals and rugged scouring troughs, and the large mud wedge of the Shandong Peninsula. In the central part of the NYS, there is a relatively flat residual shelf plain with coarser sediment deposits. This flat shelf plain has a water depth larger than 50 m and a thin layer of sediment, on which there is a large pockmark field caused by seafloor seepage. These geomorphological structures indicate that modern sedimentary processes are the main driving force controlling the sculpture of the current seafloor surface landform. Extensive strong tidal current systems and abundant sediment sources provide the critical external forces and essential conditions for the formation of seafloor geomorphology. The tectonic basement controls the macroscopic morphological shape of the NYS, but is reflected very little in the seafloor geomorphic elements. Our results provide a detailed seafloor geomorphological map of the western region of the NYS, an area that has not previously mapped and also provide a scientific framework for further research into offshore seafloor geomorphology, shelf sedimentary processes, and submarine engineering construction in this region.

An ecological optimization project (semi-closed reclamation project) was implemented to control the invasion of Spartina alterniflora, and optimize the habitat of the Chongming Dongtan wetland, in the Changjiang (Yangtze) River Estuary. After project implementation, a macrobenthic ecological survey was conducted in a natural tidal flat and a semi-closed reclamation restoration area within the Chongming Dongtan wetland from 2019 to 2020. Compared with historical data before reclamation, findings showed that the groups, numbers, and species diversity of the macrobenthos increased significantly, and the ecological optimization project resulted in good ecological benefits. In addition, compared to the natural tidal flat, the number of collected macrobenthic phyla, and the macrobenthic density and biomass were significantly lower in the restoration area. Furthermore, the biodiversity index and functional redundancy of natural tidal flats were generally higher, indicating that the community composition and function of natural tidal flats were relatively more stable. Even though the species composition differed between a number of restoration areas and natural tidal flats, there was no difference in functional diversity, indicating that the effect of restoring ecological functions in restoration areas was optimal. Among them, the biodiversity and functional redundancy of Site S2 were significantly reduced, and the ecosystem function was extremely unstable. Habitat heterogeneity, vegetation community and decreasing salinity were the main factors that affected the ecological functions of macrobenthos. The ecological quality was also evaluated; the Transects N3 and N4 showed good quality. The overall ecological quality of the restoration area was generally high, but that of Site S2 was poor and that of Site S2E was merely good, which was mainly due to modifications of the ecological function of macrobenthos. It is suggested that reeds mowing and freshwater species release should be adopted in restoration areas to improve the community function and the environmental disturbance resistance of the macrobenthos.

Based upon cruise observations broadly covering the tropical North Pacific during July−November 2017, together with data obtained from the World Ocean Circulation Experiment Hydrographic Program, this study examined the distribution of dissolved inorganic nitrogen (DIN, nitrate (${{\rm {NO}}_3^-} $)+nitrite (${{\rm {NO}}_2^-} $)), dissolved inorganic phosphorus (DIP) and related N* (nitrogen-anomaly, N*=N−16P+2.9, where N and P are the concentrations of DIN (>1.0 μmol/L) and DIP (>0.1 μmol/L)), used as an index of N₂ fixation, in the upper 1 000 m of the water column. Nutrient concentrations displayed distinct spatial variability in the upper ocean but became relatively constant at a depth of 1 000 m: they were high at low latitudes and in the eastern region, with an obvious nutricline at ~150 m (DIN, ~32.0 μmol/L; DIP, ~2.4 μmol/L) and then generally increased with depth; they decreased markedly (DIN, ~1.2 μmol/L; DIP, ~0.1 μmol/L; at ~150 m) at high latitudes and in the western region, where a nutricline was not apparent. The N* index showed significant meridional and zonal variation, with the most negative values located at low latitudes and in the eastern region (~10°N, ~150°−170°E), while becoming positive towards the northwest (the north of ~18°N, ~160°E westward). A N* concentration larger than 2.0 μmol/L which often used as an indicator of N₂ fixation, was observed between 155°E and 165°E; N* values were 2.0 μmol/L to 6.0 μmol/L at ~15°−28°N, i.e., much higher than those in the southern sector (0−2.0 μmol/L at ~5°−10°N). Zonally, N* decreased gradually from west (−2.0 μmol/L to 4.0 μmol/L, ~145°−165°E) to east (−2.0 μmol/L to −8.0 μmol/L, ~155°W) along ~10°N, which was consistent with the distribution of Trichodesmium abundance and N₂ fixation rates. Furthermore, since such region was also supplied with aeolian deposition, high N* was probably not only induced by N₂ fixation but also influenced by iron and/or nitrogen deposition.

Scyphozoan jellyfish outbreak events are drawing increasing attentions during the past decade. Elemental compositions of statoliths are helpful to understand jellyfish life history and blooming mechanisms, but very rare endeavor has been focused on the Scyphozoan class. In this work, we explored the feasibility of element analysis of Aurelia aurita (a representative Scyphozoan jellyfish outbreak species in China) which may be used as proxies of environment parameters during jellyfish living and moving. Statolith crystals of Aurelia aurita were found to be a gathering of hexahedron type trigonal needle with size of 10−50 μm long, and 5−10 μm in diameter. By using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) downhole profiling method, elements such as Ca, Sr, Mg, Na and P were found to be above the limit of detection and limit of quantification. The epidermis of statocyst could significantly impact the element analyses, so the real statolith element signal section needs to be selected based on elements and Ca profiles together with care. By laser ablated a signal spot repeatedly, the analytical uncertainty was about 3%−4% for Sr/Ca content ratio and Mg/Ca content ratio, but above 10% for other element/Ca content ratios (n=3). Based on the analysis of statolith from temperature-control cultured jellyfish, Sr/Ca content ratios among different statoliths of the same jellyfish were about 6% (n=14), demonstrating biological processes/vital effects causing small variations compared with analytical uncertainties. Therefore, Sr/Ca content ratios may be used as a potential proxy to reveal the living environment variations the Scyphozoan jellyfish has experienced, such as temperature history, which is helpful to understand jellyfish bloom mechanisms.

Hypoxia is increasingly reported off the Changjiang River Estuary with the confluence of multiple high volume nutrient sources. The Regional Ocean Modeling System coupled with a biological model was used to analyze the effect of different nutrient sources on the development of hypoxia off the Changjiang River Estuary. By comparing to observed data, our model suitably captured the regional dynamics of chlorophyll a, dissolved oxygen, and nutrient concentration. A series of sensitivity experiments were conducted to investigate the hypoxia response to the various nutrient sources, such as loading from the Changjiang River, Kuroshio and Taiwan Warm Current. Our model results indicated that nutrients from different sources significantly influenced the hypoxia off the Changjiang River Estuary, and it was mostly affected by nutrients sourced from the Kuroshio. The nutrients input from the Changjiang River had larger impacts on the hypoxia in the north of 30°N than that in the south of 30°N. The nutrients sourced from the Taiwan Strait had a least influence on the hypoxia off the Changjiang River Estuary.

The flux of settling particles in the ocean has been widely explored since 1980s due to its important role in biogenic elements cycling, especially in the transport of particulate organic carbon (POC) in the deep sea. However, research in the seamount area of the oligotrophic subtropical Northwest Pacific Ocean is lacking. In this work, two sediment traps were deployed at the foot and another two at the hillside of Niulang Guyot from August 2017 to July 2018. The magnitude and composition of particle fluxes were measured. The main factors influencing the spatial variations of the fluxes were evaluated. Our results indicated a low particulate flux from Niulang Guyot area in the Northwest Pacific Ocean, reflecting low primary productivity of the oligotrophic ocean. The total mass flux (TMF) decreased from 2.57 g/(m²·a) to 0.56 g/(m²·a) with increasing depth from 600 m to 4 850 m. A clear seasonal pattern of TMF was observed, with higher flux in summer than that in winter. The peak flux of 26.52 mg/(m²·d) occurred in August at 600 m, while the lowest value of 0.07 mg/(m²·d) was shown in February at 4 850 m. The settling particles at the deep layers had similar biochemical composition, with calcium carbonate (CaCO₃) accounting for up to 90%, followed by organic matter and opal, characteristics of Carbonate Ocean. The POC flux decreased more rapidly in the twilight layer because of faster decomposition, remineralization, and higher temperature. A small fraction of POC was transported into the deep ocean by biological pump. Particle fluxes were mainly controlled by the calcareous ballasts besides the primary productivity of the surface water. The advection may be another important factor affecting the flux in the seamount area. The combination of settled matters rich in foraminiferal tests with topography and currents may be the reason for regulating the local abundance of benthos on seamounts. Our results will fill in the knowledge gap of sedimentation flux, improve the understanding of ecosystem in Niulang Guyot area, and eventually provide data support for the optimization of regional ecological modeling.

In this paper, the intra-seasonal variability of the abyssal currents in the China Ocean Mineral Resources Association (COMRA) polymetallic nodule contact area, located in the western part of the Clarion and Clipperton Fraction Zone in the tropical East Pacific, is investigated using direct observations from subsurface mooring instruments as well as sea-surface height data and reanalysis products. Mooring observations were conducted from September 13, 2017 to August 15, 2018 in the COMRA contact area (10°N, 154°W). The results were as follows: (1) At depths below 200 m, the kinetic energy of intra-seasonal variability (20−100 d) accounts for more than 40% of the overall low-frequency variability, while the ratio reaches more than 50% below 2 000 m. (2) At depths below 200 m, currents show a synchronous oscillation with a characteristic time scale of 30 d, lasting from October to the following January; the energy of the 30-d oscillation increases with depth until the layer of approximately 4 616 m, and the maximum velocity is approximately 10 cm/s. (3) The 30-d oscillation of deep currents is correlated with the tropical instability waves in the upper ocean.

The small yellow croaker (Larimichthys polyactis) belongs to the family Sciaenidae, which is an offshore warm fish species and widely distributed in the western Pacific. In this study, the variation of genetic diversity and genetic differentiation among L. polyactis populations was analyzed by mitochondrial DNA control region. A total of 110 polymorphic sites were checked, which defined 134 haplotypes. High level of haplotype diversity (h=0.993±0.002) was detected in the examined range. Population genetic structure analyse (analysis of molecular variance, F_st) showed there were high gene flow among L. polyactis populations. The result showed that there were relatively high genetic diversity and low genetic differentiation among the Yellow Sea and the East China Sea populations, which can be attributed to diverse habitats, wide distribution range and high mutation rate of control region. Using phylogenetic methods, coalescent analyses (neutrality tests, mismatch distribution analysis, Bayesian skyline analyses) and molecular dating interpreted in conjunction with paleoclimatic and physiographic evidence, we inferred that the genetic make-up of extant populations of L. polyactis was shaped by Pleistocene environmental impacts on the historical demography of this species. Besides, relatively constant genetic diversity and larger effective population size were detected in recent L. polyactis population. The result showed that the fishing policy certainly, such as the summer closed fishing, played a role in protecting resources of L. polyactis. This study can offer a wealth of biological novelties which indicates genetic structure of L. polyactis population and provides the foundation for resources protection and policy setting.