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Point-of-care testing for lysine concentration in swine serum via blueemissive carbon dot-entrapped microfluidic chip
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Chizhu Ding, Xiang Chen, Xiaoyu Chen, Yue Liu, Menglin Xia, Ziyi He, Qinshu Kang, Xianghua Yan*
Animal Nutrition | 2023, 12(1) : 236 - 244
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Animal Nutrition | 2023, 12(1): 236-244
• Original Research Article •
Point-of-care testing for lysine concentration in swine serum via blueemissive carbon dot-entrapped microfluidic chip
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Chizhu Ding, Xiang Chen, Xiaoyu Chen, Yue Liu, Menglin Xia, Ziyi He, Qinshu Kang, Xianghua Yan*
Affiliations
  • State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Science, College of Animal Sciences and Technology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China
Published: 2023-03-10 doi: 10.1016/j.aninu.2022.08.017
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Lysine is one of the essential amino acids and plays a vital role in the growth, development and health of pigs. Blood lysine concentration is a direct indication of lysine status; however, current methods can not satisfy the demands for rapid and on-site lysine concentration measurement of swine serum. Here, we developed blue-emissive nitrogen-doped carbon dots as a fluorescence probe for the determination of lysine with high fluorescence quantum yield, stability, sensitivity and specificity. The carbon dots were entrapped within hydrogel microstructures to fabricate microfluidic chips for rapid assay for lysine quantification. We further developed an imaging attachment to integrate the microfluidic chip and a smartphone into a portable point-of-care testing platform. This platform requires only 3 μL sample and has a linear detection range of 25 to 300 μmol/L with a limit of detection less than 16 μmol/L, which covers the normal range of lysine concentration in swine serum. We tested lysine concentration in swine serum using this platform with high accuracy, low sample consumption, and within 3 min. Together, these results may provide a rapid and portable platform for dynamic monitoring of swine lysine status and contribute to precise feed formula modulation with low-protein diet strategy.

Lysine  /  Swine serum  /  Carbon dots  /  Microfluidics  /  Point-of-care testing
Chizhu Ding, Xiang Chen, Xiaoyu Chen, Yue Liu, Menglin Xia, Ziyi He, Qinshu Kang, Xianghua Yan. Point-of-care testing for lysine concentration in swine serum via blueemissive carbon dot-entrapped microfluidic chip[J]. Animal Nutrition, 2023 , 12 (1) : 236 -244 . DOI: 10.1016/j.aninu.2022.08.017
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Article Info
doi: 10.1016/j.aninu.2022.08.017
  • Receive Date：2022-05-03
  • Online Date：2026-02-03
  • Published：2023-03-10
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History
  • Received：2022-05-03
  • Revised：2022-06-16
  • Accepted：2022-08-13
Affiliations
    State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Frontiers Science Center for Animal Breeding and Sustainable Production, College of Science, College of Animal Sciences and Technology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China

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* Corresponding author. E-mail address: (X. Yan).
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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