中医药学报

补肾壮筋汤对肾虚血瘀型膝骨性关节炎大鼠软骨损伤的保护作用与分子机制探讨

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李新 ¹^,² , 史鹏博 ¹

中医药学报 | 实验研究 2026,54(4): 16-23

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中医药学报 |实验研究 2026 , 54 (4) : 16 -23

补肾壮筋汤对肾虚血瘀型膝骨性关节炎大鼠软骨损伤的保护作用与分子机制探讨

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李新¹^,², 史鹏博¹

作者信息

^1.河南中医药大学第一附属医院，河南郑州 450099

^2.河南中医药大学，河南郑州 450046

作者简介:

李新（1983-），女，副主任医师，研究方向：腰椎管狭窄症的中医保守治疗。

Exploration of the Protective Effect and Molecular Mechanism of Bushen Zhuangjin Decoction on Cartilage Injury in Rats with Knee Osteoarthritis of Kidney Deficiency and Blood Stasis Type

Xin LI¹^,², Pengbo SHI¹

Affiliations

^1.First Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou 450099, China

^2.Henan University of Chinese Medicine, Zhengzhou 450046, China

出版时间: 2026-04-20 doi: 10.19664/j.cnki.1002-2392.260070

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摘要

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目的：

观察补肾壮筋汤对肾虚血瘀型膝骨性关节炎（KOA）大鼠软骨损伤的保护作用，并探讨其分子机制。

方法：

70只雌性SD大鼠采用随机数字表法分为正常组，模型组，阳性药组，补肾壮筋汤低、中、高剂量组，SB203580组，每组10只。除正常组外，其余均建立肾虚血瘀型KOA模型。模型组与正常组中随机抽取1只处死验证模型，剩余大鼠中阳性药组予以依托考昔5.4 mg/kg灌胃，补肾壮筋汤低、中、高剂量组分别予以补肾壮筋汤2.1、4.2、8.4 g/kg灌胃，SB203580组灌胃SB203580 3.0 mg/kg，模型组予以1 mL/100 g生理盐水灌胃。正常组10只灌胃等体积生理盐水。干预30 d，酶联免疫法检测各组关节液肿瘤坏死因子- α（TNF-α）、白介素-1β（IL-1β）、白介素- 6（IL-6）水平；苏木素-伊红（HE）染色观察各组软骨组织病理改变，比较Mankin评分；实时-逆转录聚合酶链反应（RT-qPCR）检测软骨组织p38丝裂原活化蛋白激酶（p38MAPK）、核转录因子- κB p65（NF-κB p65）、核苷酸结合寡聚化结构域样受体3（NLRP3）、天冬氨酸蛋白水解酶3（Caspase3）基因表达水平；免疫印迹法（WB）检测软骨组织p38MAPK、NF-κB p65、NLRP3、Caspase3蛋白表达及p-p38MAPK水平。

结果：

模型组大鼠关节液TNF-α、IL-1β、IL-6水平，Mankin评分，膝关节软骨组织p38MAPK基因、NF-κB p65、NLRP3和Caspase3基因和蛋白表达水平以及p-p38MAPK均高于正常组（P < 0.01），各治疗组均低于模型组（P < 0.01），除Caspase3表达外补肾壮筋汤高剂量组均低于SB203580组（P < 0.01），且补肾壮筋汤的作用呈剂量依赖性；模型组大鼠软骨组织呈严重病理改变，各治疗组均减轻，补肾壮筋汤低剂量组与阳性药组、补肾壮筋汤中剂量组及SB203580组的作用均基本相当。

结论：

补肾壮筋汤可减轻肾虚血瘀型KOA大鼠软骨组织炎症反应，推测可通过抑制p38MAPK/NF-κB通路实现此作用。

关键词

补肾壮筋汤 / 膝骨性关节炎 / 肾虚血瘀 / 软骨损伤

Abstract

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Objective:

To observe the protective effect of Bushen Zhuangjin Decoction on cartilage damage in knee osteoarthritis (KOA) rats with kidney deficiency and blood stasis type, and to explore its molecular mechanism.

Methods:

Seventy female SD rats were divided into 7 groups by a random digital table, with 10 in each. Except the normal group, the others were established kidney deficiency and blood stasis type KOA models. Each 1 rat was selected from the model group and the normal group, which were euthanized to verify successful modeling. Among the others, the positive drug group was given a dose of 5.4 mg/kg of etoposide by gavage. The low, medium, and high dose groups of Bushen Zhuangjin Decoction were given Bushen Zhuangjin Decoction 2.1, 4.2, and 8.4 g/kg by gavage respectively. SB203580 group was orally administered with SB203580 3.0 mg/kg. The model group was given 1 mL/100 g of physiological saline by gavage. The other 10 female SD rats were given an equal volume of physiological saline by gavage (normal group). All lasted for 30 days. Enzyme linked immunosorbent assay was used for detecting tumor necrosis factor-α (TNF)-α, interleukin-1β (IL-1β) and interleukin-6 (IL-6) levels in joint fluid. Hematoxylin eosin (HE) staining was used to observe the pathological changes of cartilage tissues, and the Mankin scores were compared. Real time reverse transcription polymerase chain reaction (RT-qPCR) was used to detect p38 mitogen activated protein kinase (p38MAPK) and nuclear transcription factors in various groups of cartilage tissues-κB p65 (NF-κB p65), nucleotide binding oligomerization domain like receptor 3 (NLRP3), and aspartate proteolytic enzyme 3 (Caspase3) gene expressions. Western blotting (WB) was used for detecting p38MAPK and NF-κB p65, NLRP3, Caspase3 protein expressions and p-p38MAPK levels in cartilage tissues.

Results:

KOA models of kidney deficiency and blood stasis type were successfully established. The TNF-α, IL-1β, IL-6 levels of joint fluid, Mankin score, p38MAPK gene, NF-κB p65, NLRP3, Caspase3 expressions and p-p38MAPK in knee cartilage tissues in the model group were all higher than those in the normal group (P <0.01), of which the treatment groups were all lower than the model group (P <0.01), and except for Caspase3 expressions the indexes in the high-dose group of Bushen Zhuangjin Decoction were lower than the SB203580 group (P <0.01), and the effect of Bushen Zhuangjin Decoction was dose-dependent. The cartilage tissue of the model group rats showed severe pathological changes, and the treatment groups all showed relief, and the effects of the low-dose and positive drug groups of Bushen Zhuangjin Decoction, the medium dose group of Bushen Zhuangjin Dectoction and SB203580 group were basically equivalent.

Conclusion:

Bushen Zhuangjin Decoction can alleviate the inflammatory response of cartilage tissue in KOA rats with kidney deficiency and blood stasis type, and it is speculated to inhibit p38MAPK/NF-κB pathway achieves this effect.

Key words

Bushen Zhuangjin Decoction / Knee osteoarthritis / Kidney deficiency and blood stasis / Cartilage injury

引用本文

李新, 史鹏博. 补肾壮筋汤对肾虚血瘀型膝骨性关节炎大鼠软骨损伤的保护作用与分子机制探讨. 中医药学报, 2026 , 54 (4) : 16 -23 . DOI: 10.19664/j.cnki.1002-2392.260070

Xin LI, Pengbo SHI. Exploration of the Protective Effect and Molecular Mechanism of Bushen Zhuangjin Decoction on Cartilage Injury in Rats with Knee Osteoarthritis of Kidney Deficiency and Blood Stasis Type[J]. Acta Chinese Medicine and Pharmacology, 2026 , 54 (4) : 16 -23 . DOI: 10.19664/j.cnki.1002-2392.260070

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2023年度河南省中医药科学研究专项课题(2023ZY2010)

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2026年第54卷第4期

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doi: 10.19664/j.cnki.1002-2392.260070

接收时间：2025-03-06
首发时间：2026-09-17
出版时间：2026-04-20

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收稿日期：2025-03-06
修回日期：2025-12-11

基金

2023年度河南省中医药科学研究专项课题(2023ZY2010)

作者信息

^1.河南中医药大学第一附属医院，河南郑州 450099

^2.河南中医药大学，河南郑州 450046

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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