中医药学报

基于宏基因组学探讨扶正消癌Ⅰ号方对结直肠癌荷瘤裸鼠肠道菌群的影响

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肖文娟 ¹ , 张苗 ¹ , 宋雅楠 ² , 夏伟 ¹^,²^,^*

中医药学报 | 实验研究 2026,54(4): 7-15

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中医药学报 |实验研究 2026 , 54 (4) : 7 -15

基于宏基因组学探讨扶正消癌Ⅰ号方对结直肠癌荷瘤裸鼠肠道菌群的影响

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肖文娟¹, 张苗¹, 宋雅楠², 夏伟¹^,²^,^*

作者信息

^1.上海中医药大学附属第七人民医院，上海 200137

^2.上海市浦东新区公利医院，上海 200135

通讯作者:

^* 夏伟（1976-），男，博士，教授，主要从事肿瘤核医学诊疗。

作者简介:

肖文娟（1999-），女，硕士研究生，主要从事肿瘤的中西医结合治疗研究。

Metagenomics-Based Study on Effects of Fuzheng Xiao'ai Formula I on Intestinal Flora in Colorectal Tumor-Bearing Nude Mice

Wenjuan XIAO¹, Miao ZHANG¹, Yanan SONG², Wei XIA¹^,²^,^*

Affiliations

^1.The Seventh People's Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200137, China

^2.Gongli Hospital of Pudong New District, Shanghai 200135, China

出版时间: 2026-04-20 doi: 10.19664/j.cnki.1002-2392.260069

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摘要

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目的：

从肠道菌群角度研究扶正消癌Ⅰ号方对结直肠癌（CRC）裸鼠肿瘤的抑制作用。

方法：

构建CRC裸鼠模型，将造模成功的20只裸鼠随机分为模型对照组和扶正消癌Ⅰ号方低（17.47 g/kg）、中（34.94 g/kg）、高（69.89 g/kg）剂量组，每组5只。模型对照组灌胃生理盐水，扶正消癌Ⅰ号方各剂量组灌胃对应浓度水煎液，连续干预2周后处死裸鼠，完整剥离瘤体并取部分肿瘤组织进行HE染色。基于瘤重、体积及病理结果筛选出疗效较好的中剂量组，收集该组及模型对照组的盲肠内容物进行宏基因组测序分析。

结果：

扶正消癌Ⅰ号方各剂量组裸鼠的瘤重和体积均下降（P <0.05，P <0.01）。HE染色显示扶正消癌Ⅰ号方低、中、高剂量组的肿瘤细胞密度降低，且伴有不同程度坏死。宏基因组学测序显示，与模型对照组相比，扶正消癌Ⅰ号方中剂量组的副萨特氏菌属、双歧杆菌属、粪杆菌属、耳蜗形梭菌、啮齿粪杆菌丰度显著增加（P <0.05），变形杆菌属、柠檬酸杆菌属、摩根菌属、肠杆菌属、摩氏摩根菌、霍氏肠杆菌的丰度显著降低（P <0.05）。京都基因与基因组百科全书（KEGG）分析发现淀粉与蔗糖代谢通路在两组中差异显著（P <0.05），其中α-葡萄苷酶、UTP-葡萄糖-1-磷酸尿苷酸转移酶、磷酸葡萄糖变位酶、麦芽糖α-D -葡萄糖基转移酶在该通路中明显下调（P <0.05）。

结论：

扶正消癌Ⅰ号方通过增加有益菌的丰度，降低有害菌的丰度，进而修复肠道屏障，维持肠道内环境的稳态，而下调淀粉和蔗糖代谢通路中的α -葡糖苷酶可能是其发挥抗CRC的潜在途径。

关键词

结直肠癌 / 扶正消癌Ⅰ号方 / 肠道菌群 / 宏基因组学 / α -葡萄糖苷酶

Abstract

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Objective:

To study the inhibitory effect of Fuzheng Xiao'ai Formula I on colorectal cancer tumors in nude mice from the perspective of intestinal flora.

Methods:

The CRC nude mouse model was constructed and 20 successfully modeled nude mice were randomLy divided into the model control group and low dose (17.47 g/kg), medium dose (34.94 g/kg), and high dose (69.89 g/kg) groups of Fuzheng Xiao'ai Formula I, with 5 mice in each group. The model control group was gavaged with normal saline, and each dose of Fuzheng Xiao'ai Formula I was gavaged with the corresponding concentration of decoction. After 2 consecutive weeks of intervention, the nude mice were sacrificed, the tumor bodies were completely dissected, and some tumor tissues were taken for HE staining. Based on the tumor weight, volume and pathological results, the medium dose group with better efficacy was screened out. Meanwhile, the cecal contents of this group and the model control group were collected for metagenomic sequencing analysis.

Results:

The tumor weight and volume of nude mice in each dose group of Fuzheng Xiao'ai Formula I decreased. HE staining showed that the density of tumor cells in the low, medium, and high dose groups of Fuzheng Xiao'ai Formula I decreased, accompanied by different degrees of necrosis. Metagenomic sequencing showed that compared with the model control group, the abundances of Parabacteroides, Bifidobacterium, Faecalibacterium, Clostridium auriscati, and Faecalibaculum rodentium in the medium dose group significantly increased (P < 0.05), and the abundances of Proteus, Citrobacter, Morganella, Enterobacter, Morganella morganii, and Enterobacter hormaechei significantly decreased (P < 0.05). Kyoto Encyclopedia of Genes and Genomes analysis found that the starch and sucrose metabolism pathway was significantly different between the two groups (P < 0.05), among which α-glucosidase, UTP-glucose-1-phosphate uridylyltransferase, phosphoglucomutase, and maltose α-D-glucosyltransferase were significantly downregulated in this pathway (P <0. 05).

Conclusion:

Fuzheng Xiao'ai Formula I repairs the intestinal barrier and maintains the homeostasis of the intestinal environment by increasing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria. Down-regulating α-glucosidase in the starch and sucrose metabolism pathway may be a potential way for it to exert anti-colorectal cancer effects.

Key words

Colorectal cancer / Fuzheng Xiao'ai Formula Ⅰ / Intestinal flora / Metagenomics / α-Glucosidase

引用本文

肖文娟, 张苗, 宋雅楠, 夏伟. 基于宏基因组学探讨扶正消癌Ⅰ号方对结直肠癌荷瘤裸鼠肠道菌群的影响. 中医药学报, 2026 , 54 (4) : 7 -15 . DOI: 10.19664/j.cnki.1002-2392.260069

Wenjuan XIAO, Miao ZHANG, Yanan SONG, Wei XIA. Metagenomics-Based Study on Effects of Fuzheng Xiao'ai Formula I on Intestinal Flora in Colorectal Tumor-Bearing Nude Mice[J]. Acta Chinese Medicine and Pharmacology, 2026 , 54 (4) : 7 -15 . DOI: 10.19664/j.cnki.1002-2392.260069

基金

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国家自然科学基金项目(82204845)
上海市浦东新区卫生系统重点学科建设项目(PWZxk2022-04)
上海市科学技术委员会科技创新行动计划(22ZR1447900)

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排序方式：

2026年第54卷第4期

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文章信息

doi: 10.19664/j.cnki.1002-2392.260069

接收时间：2025-01-07
首发时间：2026-09-17
出版时间：2026-04-20

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出版历史

收稿日期：2025-01-07
修回日期：2025-09-12

基金

国家自然科学基金项目(82204845)

上海市浦东新区卫生系统重点学科建设项目(PWZxk2022-04)

上海市科学技术委员会科技创新行动计划(22ZR1447900)

作者信息

^1.上海中医药大学附属第七人民医院，上海 200137

^2.上海市浦东新区公利医院，上海 200135

通讯作者:

^* 夏伟（1976-），男，博士，教授，主要从事肿瘤核医学诊疗。

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https://castjournals.cast.org.cn/joweb/zyyxb/CN/10.19664/j.cnki.1002-2392.260069

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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