中医药学报

小青龙汤通过激活自噬与抑制IL-33/ ST2通路治疗变应性鼻炎的作用及机制研究

PDF下载

李云松 ¹ , 杨荣刚 ¹^,^* , 梁友情 ²

中医药学报 | 实验研究 2026,54(8): 6-13

收起

中医药学报 |实验研究 2026 , 54 (8) : 6 -13

小青龙汤通过激活自噬与抑制IL-33/ ST2通路治疗变应性鼻炎的作用及机制研究

全屏

[Author(id=1307264801772691805, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307264801848189279, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, authorId=1307264801772691805, language=EN, stringName=Yunsong LI, firstName=Yunsong, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307264801919492448, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, authorId=1307264801772691805, language=CN, stringName=李云松, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.贵州中医药大学，贵州贵阳 550025, bio={"content":"

李云松（1990-），男，硕士，主治医师，主要从事中西医结合治疗耳鼻喉疾病的研究。

"}, bioImg=null, bioContent=

李云松（1990-），男，硕士，主治医师，主要从事中西医结合治疗耳鼻喉疾病的研究。

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307264801604919638, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, xref=1., ext=[AuthorCompanyExt(id=1307264801613308247, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, companyId=1307264801604919638, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^1.Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China), AuthorCompanyExt(id=1307264801621696856, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, companyId=1307264801604919638, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^1.贵州中医药大学，贵州贵阳 550025)])]), Author(id=1307264801978212706, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1307264802049515876, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, authorId=1307264801978212706, language=EN, stringName=Ronggang YANG, firstName=Ronggang, middleName=null, lastName=YANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹^,^*, address=^1.Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307264802120819045, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, authorId=1307264801978212706, language=CN, stringName=杨荣刚, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹^,^*, address=^1.贵州中医药大学，贵州贵阳 550025, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307264801604919638, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, xref=1., ext=[AuthorCompanyExt(id=1307264801613308247, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, companyId=1307264801604919638, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^1.Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China), AuthorCompanyExt(id=1307264801621696856, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, companyId=1307264801604919638, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^1.贵州中医药大学，贵州贵阳 550025)])]), Author(id=1307264802183733607, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1307264802246648169, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, authorId=1307264802183733607, language=EN, stringName=Youqing LIANG, firstName=Youqing, middleName=null, lastName=LIANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=², address=^2.The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1307264802301174122, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, authorId=1307264802183733607, language=CN, stringName=梁友情, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=², address=^2.贵州中医药大学第二附属医院，贵州贵阳 550001, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1307264801680417113, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, xref=2., ext=[AuthorCompanyExt(id=1307264801688805722, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, companyId=1307264801680417113, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^2.The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, China), AuthorCompanyExt(id=1307264801697194331, tenantId=1146029695717560320, journalId=1302318977290174537, articleId=1307264800841556307, companyId=1307264801680417113, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=^2.贵州中医药大学第二附属医院，贵州贵阳 550001)])])]

李云松¹, 杨荣刚¹^,^*, 梁友情²

作者信息

^1.贵州中医药大学，贵州贵阳 550025

^2.贵州中医药大学第二附属医院，贵州贵阳 550001

通讯作者:

^* 杨荣刚（1977-），男，博士，副教授，副主任医师，主要从事中医药对耳鼻喉疾病的研究。

作者简介:

李云松（1990-），男，硕士，主治医师，主要从事中西医结合治疗耳鼻喉疾病的研究。

Therapeutic Effect and Mechanism of Xiaoqinglong Decoction in Treating Allergic Rhinitis via Activating Autophagy and Inhibiting the IL-33/ST2 Pathway

Yunsong LI¹, Ronggang YANG¹^,^*, Youqing LIANG²

Affiliations

^1.Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China

^2.The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550001, China

出版时间: 2026-08-20 doi: 10.19664/j.cnki.1002-2392.260154

文章导航

摘要

收起

目的：

探讨小青龙汤通过自噬与IL -33/ST2通路治疗变应性鼻炎的可能作用机制。

方法：

采用卵清蛋白（OVA）致敏建立变应性鼻炎小鼠模型。24只小鼠随机分为对照组、模型组、地塞米松组及小青龙汤组。除对照组外，其余各组第1、7、14天腹腔注射含50 μg OVA与2 mg氢氧化铝的PBS溶液致敏；第21 ~ 27天每24 h鼻腔滴注400 μg OVA激发（共4次）。小青龙汤组给予小青龙汤（10.14 g/kg），地塞米松组给予地塞米松磷酸二钠（1 mg/kg），模型组和对照组给予对应量PBS，采用灌胃给药1次/d，共7 d。末次激发24 h后，通过双盲法评估鼻部症状（喷嚏、鼻痒、鼻涕）。采集血清应用ELISA法检测OVA-sIgE及Th1/Th2细胞因子（IL-2、IL-12、IL-5、IL-13），取鼻黏膜组织行HE染色及IL-2、IL-12、IL-5、IL-13、IL-33、ST2、Beclin1、P62免疫组化分析。

结果：

与对照组比较，模型组小鼠症状评分显著升高（P <0.01），鼻黏膜呈现上皮剥脱、血管扩张和腺体增生并伴有大量炎症浸润，血清OVA-sIgE、IL-5、IL-13水平显著升高（P <0.01），IL-2、IL-12水平降低（P <0.01），鼻黏膜组织IL-5、IL-13、IL-33、ST2、P62蛋白表达升高（P <0.01），IL-2、IL-12、Beclin1表达降低（P <0.01）。与模型组比较，小青龙汤组及地塞米松组小鼠症状评分显著降低（P <0.01），鼻黏膜病理改善，血清OVA-sIgE、IL-5、IL-13降低（P <0.01）而IL-2、IL-12升高（P <0.01），鼻黏膜组织IL-2、IL-12、Beclin1蛋白表达均升高（P <0.01），而IL-5、IL-13、IL -33、ST2、P62蛋白表达均降低（P <0.01）。与地塞米松组比较，小青龙汤组小鼠症状评分，血清IL-2、IL-12、IL-5、OVA-sIgE含量，鼻黏膜组织IL-2、IL-12、IL-5、IL-13、Beclin1和P62蛋白表达差异均无统计学意义（P >0.05）。

结论：

小青龙汤可以通过激活细胞自噬与抑制IL -33/ST2通路协同调节Th1/Th2免疫平衡显著缓解变应性鼻炎小鼠鼻部症状及鼻黏膜病理损伤。

关键词

小青龙汤 / 变应性鼻炎 / 自噬 / IL -33/ST2通路 / Th1/Th2平衡

Abstract

收起

Objective:

To investigate the potential mechanism of Xiaoqinglong Decoction (XQLD) in treating allergic rhinitis (AR) through autophagy and the IL -33/ST2 pathway.

Methods:

An ovalbumin (OVA)-sensitized mouse model of allergic rhinitis was established. Twenty-four mice were randomly assigned to four groups: control group, model group, dexamethasone (Dex) group, and XQLD group. All groups except the control received intraperitoneal injections of PBS containing 50 μg OVA and 2 mg aluminum hydroxide on days 1, 7, and 14 for sensitization. From days 21 to 27, mice were challenged intranasally with 400 μg OVA every 24 hours (4 total challenges). Interventions: The XQLD group received XQLD (10. 14 g/kg), the dexamethasone group received dexamethasone disodium phosphate (1 mg/kg), while the model and control groups received equivalent volumes of PBS. All treatments were administered once daily via gavage for 7 days. At 24 h after the final challenge, nasal symptoms (sneezing, scratching, rhinorrhea) were evaluated using a double-blind protocol. Serum was collected for the detection of OVA-specific IgE (OVA-sIgE) and Th1/Th2 cytokines (IL-2, IL-12, IL-5, IL-13) using ELISA. Nasal mucosa tissues underwent Hematoxylin and Eosin (H&E) staining, and immunohistochemical analysis was performed for IL-2, IL-12, IL-5, IL-13, IL-33, ST2, Beclin1, and P62.

Results:

compared to the control group, mice in the model group exhibited significantly elevated symptom scores (P < 0.01). The nasal mucosa showed pathological changes including epithelial desquamation, vasodilation, glandular hyperplasia, and extensive inflammatory cell infiltration. Serum OVA-sIgE, IL-5, and IL-13 levels were significantly increased (P < 0.01), while IL-2 and IL-12 levels were decreased (P < 0.01). In nasal mucosa tissue, the protein expression of IL-5, IL-13, IL-33, ST2, and P62 was elevated (P < 0.01), whereas the expression of IL-2, IL-12, and Beclin1 was decreased (P < 0.01). Compared to the model group, mice treated with XQLD and Dex groups showed significantly reduced symptom scores (P < 0.01) and improved nasal mucosal pathology. Serum OVA-sIgE, IL-5, and IL-13 levels decreased (P <0.01), while IL-2 and IL-12 levels increased (P <0.01). In nasal mucosa tissue, the protein expression of IL-2, IL-12, and Beclin1 increased (P <0.01), while the expression of IL-5, IL-13, IL-33, ST2, and P62 decreased (P < 0.01). No statistically significant differences were observed between the XQLD group and the Dex group regarding symptom scores, serum IL-2, IL-12, IL-5, OVA- sIgE levels, and nasal mucosa tissue IL-2, IL-12, IL-5, IL-13, Beclin1, and P62 protein expression (P >0.05).

Conclusion:

XQLD alleviates nasal symptoms and pathological damage in AR mice by synergistically regulating Th1/Th2 immune balance through autophagy activation and IL -33/ST2 pathway inhibition.

Key words

Xiaoqinglong Decoction / Allergic rhinitis / Autophagy / IL -33/ST2 pathway / Th1/Th2 balance

引用本文

李云松, 杨荣刚, 梁友情. 小青龙汤通过激活自噬与抑制IL-33/ ST2通路治疗变应性鼻炎的作用及机制研究. 中医药学报, 2026 , 54 (8) : 6 -13 . DOI: 10.19664/j.cnki.1002-2392.260154

Yunsong LI, Ronggang YANG, Youqing LIANG. Therapeutic Effect and Mechanism of Xiaoqinglong Decoction in Treating Allergic Rhinitis via Activating Autophagy and Inhibiting the IL-33/ST2 Pathway[J]. Acta Chinese Medicine and Pharmacology, 2026 , 54 (8) : 6 -13 . DOI: 10.19664/j.cnki.1002-2392.260154

基金

收起

国家自然科学基金项目(82260955)
贵州省中医药管理局科研项目(QZYY-2020-003)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

2026年第54卷第8期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.19664/j.cnki.1002-2392.260154

接收时间：2025-07-03
首发时间：2026-09-17
出版时间：2026-08-20

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2025-07-03
修回日期：2026-03-12

基金

国家自然科学基金项目(82260955)

贵州省中医药管理局科研项目(QZYY-2020-003)

作者信息

^1.贵州中医药大学，贵州贵阳 550025

^2.贵州中医药大学第二附属医院，贵州贵阳 550001

通讯作者:

^* 杨荣刚（1977-），男，博士，副教授，副主任医师，主要从事中医药对耳鼻喉疾病的研究。

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zyyxb/CN/10.19664/j.cnki.1002-2392.260154

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT