中医药学报

黄蓉五仁膏治疗慢性传输型便秘的毒理及药效学研究

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周雪 ¹ , 路越 ²^,^*

中医药学报 | 实验研究 2026,54(9): 19-26

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中医药学报 |实验研究 2026 , 54 (9) : 19 -26

黄蓉五仁膏治疗慢性传输型便秘的毒理及药效学研究

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周雪¹, 路越²^,^*

作者信息

^1．辽宁中医药大学，辽宁沈阳 110847

^2．辽宁中医药大学附属第三医院，辽宁沈阳 110003

通讯作者:

^* 路越（1980 -），男，硕士，主任医师，硕士生导师，从事肛肠疾病的基础与临床研究。

作者简介:

周雪（2000-），女，硕士研究生，专业：中医外科学。

Toxicological and Pharmacodynamic Studies of Huangrong Wuren Paste in the Treatment of Slow Transit Constipation

Xue ZHOU¹, Yue LU²^,^*

Affiliations

^1.Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China

^2.The Third Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110003, China

出版时间: 2026-09-20 doi: 10.19664/j.cnki.1002-2392.260174

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摘要

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目的：

观察黄蓉五仁膏治疗慢性传输型便秘的有效性及安全性。

方法：

药效学研究中，将36只小鼠随机分为空白组、模型组、乳果糖组（10 mg/kg），黄蓉五仁膏低（2 515 mg/kg）、中（5 030 mg/kg）、高（10 060 mg/kg）剂量组，每组6只，雌雄各半。除空白组外均给予盐酸罗哌丁胺（10 mg/kg）经口灌胃制备小鼠便秘模型，空白组给予等体积的生理盐水，1次/d，连续14 d。造模成功后，给予黄蓉五仁膏进行治疗，1次/d，连续14 d。观察小鼠一般状态，检测首粒黑便排出时间、粪便颗粒数及含水率和肠道水分含量。毒理学研究分为急性经口试验、细菌回复突变试验、哺乳动物红细胞微核试验、精原细胞染色体畸变试验，检测其相应指标。

结果：

①与乳果糖组比较，黄蓉五仁膏中、高剂量组首粒黑便排出时间明显缩短（P < 0.05），且时间随黄蓉五仁膏浓度的增加而缩短。②与模型组和乳果糖组相比，黄蓉五仁膏高剂量组小鼠的肠道碳墨推进率皆明显增加（P < 0.05，P < 0.01）。③与模型组比较，黄蓉五仁膏高剂量组小鼠肠道水分含量明显增加（P < 0.01）。④毒理实验条件下，小鼠一般状态良好，未出现中毒表现及死亡情况，处死解剖后，内脏器官未见病变表现；菌株TA1535的回变菌落数是未处理对照组回变菌落数的3倍以下；其余菌株回变菌落数是未处理对照组回变菌落数的 2倍以下；骨髓PCE微核发生率未出现增高；小鼠精原细胞未产生畸变反应。

结论：

黄蓉五仁膏治疗慢性传输型便秘具有明显的疗效，且具有较高的安全性，未见生殖毒性。

关键词

慢性传输型便秘 / 黄蓉五仁膏 / 药效学研究 / 毒理学研究

Abstract

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Objective:

To observe the efficacy and safety of Huangrong Wuren Paste in the treatment of slow transit constipation.

Methods:

In the pharmacodynamic study, 36 mice were randomly divided into blank group, model group, lactulose group (10 mg/kg), Huangrong Wuren Paste low (2 515 mg/kg), medium (5 030 mg/kg), high (10 060 mg/kg) dose groups, 6 mice in each group, half male and half female. The constipation model was prepared by oral gavage of loperamide hydrochloride at 10mg/kg in all groups except the blank group. The blank group was given equal volume of saline once a day for 14 days. After the model was built, Huangrong Wuren Paste was given for treatment once a day for 14 days. Observe the general state of the mice, the time of first melena excretion, the number and moisture content of faeces, and the intestinal moisture content. Toxicological studies were divided into Acute Oral Toxicity Test, Bacterial Reverse Mutation Assay, Mammalian Erythrocyte Micronucleus Test and Spermatogonia Chromosome Aberration Assay. Observe the corresponding indicators.

Results:

①Compared with lactulose group, the melena excretion time of mice in low, all groups of Huangrong Wuren Paste was significantly shortened, and the time was shortened with the increase of the concentration of Huangrong Wuren Paste. ②Compared with both model group and lactulose group, the propellant rate of carbon powder in Huangrong Wuren Paste high-dose group was significantly increased (P < 0. 05).③Compared with model group, the intestinal moisture content in high-dose Huangrong Wuren Paste group was significantly increased (P <0. 01). ④Under the toxicological studies, the mice were generally in good condition, and there were no signs of poisoning and death. After the dissection, there were no signs of lesions in the organs. The number of revertant colonies for strain TA1535 was less than three times that of the untreated control group, the number of revertant colonies for the remaining strains was less than twice that of untreated control group. the incidence of bone marrow PCE micronucleus did not increase and the spermatogonial cells did not exhibit any aberrant reactions.

Conclusion:

Huangrong Wuren Paste has obvious curative effect and high safety on slow transit constipation, with no reproductive toxicity.

Key words

Slow transit constipation / Huangrong Wuren Paste / Pharmacodynamic study / Toxicological study

引用本文

周雪, 路越. 黄蓉五仁膏治疗慢性传输型便秘的毒理及药效学研究. 中医药学报, 2026 , 54 (9) : 19 -26 . DOI: 10.19664/j.cnki.1002-2392.260174

Xue ZHOU, Yue LU. Toxicological and Pharmacodynamic Studies of Huangrong Wuren Paste in the Treatment of Slow Transit Constipation[J]. Acta Chinese Medicine and Pharmacology, 2026 , 54 (9) : 19 -26 . DOI: 10.19664/j.cnki.1002-2392.260174

基金

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国家自然科学基金面上项目(82174371)

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排序方式：

2026年第54卷第9期

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文章信息

doi: 10.19664/j.cnki.1002-2392.260174

接收时间：2025-03-28
首发时间：2026-09-11
出版时间：2026-09-20

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收稿日期：2025-03-28
修回日期：2025-12-03

基金

国家自然科学基金面上项目(82174371)

作者信息

^1．辽宁中医药大学，辽宁沈阳 110847

^2．辽宁中医药大学附属第三医院，辽宁沈阳 110003

通讯作者:

^* 路越（1980 -），男，硕士，主任医师，硕士生导师，从事肛肠疾病的基础与临床研究。

参考文献

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https://castjournals.cast.org.cn/joweb/zyyxb/CN/10.19664/j.cnki.1002-2392.260174

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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