Article(id=1304438791691857968, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1304438786377674789, articleNumber=null, orderNo=null, doi=10.19664/j.cnki.1002-2392.260004, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1731600000000, receivedDateStr=2024-11-15, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788932043240, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788932043240, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788932043240, creator=13701087609, updateTime=1788932043240, updator=13701087609, issue=Issue{id=1304438786377674789, tenantId=1146029695717560320, journalId=1302318977290174537, year='2026', volume='54', issue='1', pageStart='1', pageEnd='128', issueExtLink='null', onlineDate='null', pubDate='1768838400000', pubDateStr='2026-01-20', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788932041973, creator='13701087609', updateTime=1788934739716, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304450101590651052, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1304438786377674789, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304450101590651053, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1304438786377674789, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=19, endPage=25, ext={EN=ArticleExt(id=1304438791989653554, articleId=1304438791691857968, tenantId=1146029695717560320, journalId=1302318977290174537, language=EN, title=Effect of Dahuang Lingxian Formula on the Intestinal Microbiota in Guinea Pig Model of Gallstones, columnId=null, journalTitle=Acta Chinese Medicine and Pharmacology, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective: To investigate the impact of Dahuang Lingxian Formula (Rheum Officinale and Radix Clematidis Formula, DHLX) on the intestinal microbiota's structure and function in guinea pigs suffering from gallbladder stones.Methods: Fifty guinea pigs were randomly assigned to a blank group, a model group, and a traditional Chinese medicine(TCM) group. The model group consumed a high - fat diet to induce gallbladder stones, while the TCM group received DHLX for 8 weeks alongside the same high - fat diet. Colon fecal samples were collected at the 8th week and subjected to 16S rDNA sequencing for analyzing intestinal flora composition, diversity, and functional predictions.Results: ①The OTU count was 4623 in the normal group, 3970 in the model group, and 4335 in the TCM group; 474 OTUs were common to all three groups. ② Major dominant phyla encompassed Bacteroidetes, Firmicutes, Proteobacteria, Verrucomicrobia, and Spirochaetes. In the model group, Bacteroidetes and Verrucomicrobia increased, while Firmicutes and Spirochaetes decreased. Conversely, in the TCM group, Bacteroidetes, Firmicutes, and Verrucomicrobia decreased, while Proteobacteria and Spirochaetes increased. ③ The intestinal flora diversity index (Ace, Chao1, Shannon, Simpson) showed no significant difference between groups. ④ The intestinal flora distribution of the model group significantly diverged from the normal group, with partial overlap observed between the TCM and model groups.⑤In the blank group, Ruminococcus, Rikenellaceae, and other genera were highly expressed; Paludibacteraceae and Verrucomicrobia were prominent in the model group; Alphaproteobacteria and Desulfovibrio were highly expressed in the TCM group. ⑥The intestinal microbiota of the blank group exhibited anaerobic and biofilm synthesis traits. In the model group, Gram - negative bacteria increased, while mobile genetic elements and biofilm synthesis decreased. However, in the TCM group, the characteristics of Gram - negative bacteria and biofilm synthesis were restored.Conclusion: The intestinal microbiota structure undergoes significant changes during gallbladder stone formation, and DHLX effectively regulates the flora, restoring it to a normal steady state., authors=CHEN Jinmei1,2 , GAN Yirong3 , YU Yuan1 , FU Jun1 , QIN Yi1 , TANG Qianli2 , authorsList=CHEN Jinmei, GAN Yirong, YU Yuan, FU Jun, QIN Yi, TANG Qianli, authorCompany=1 The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530299, China; 目的： 探讨大黄灵仙方对胆囊结石豚鼠模型肠道菌群结构与功能的影响。 方法： 50只豚鼠按随机数字表法分为空白组15只、模型组20只、中药组15只。空白组给予豚鼠正常饲料喂养，每日予以0.9%生理盐水灌胃；模型组给予高脂致石饲料喂养，每日予以0.9%生理盐水灌胃；中药组给予高脂致石饲料喂养，每日予以1 g/mL大黄灵仙方灌胃。共干预8周。收集第8周的结肠粪便，利用16SrDNA测序分析肠道菌群的组成、多样性及功能预测。 结果： ①空白组OTUs数为4 623，模型组为3 970，中药组为4 335；3组共有OTUs为474；②主要优势菌门包括Bacteroidetes、Firmicutes、Proteobacteria、Verrucomicrobia和Spirochaetes；模型组中Bacteroidetes和Verrucomicrobia增加，而Firmicutes和Spirochaetes减少，中药组Bacteroidetes、Firmicutes、Verrucomicrobia减少，但Proteobacteria和Spirochaetes增加；③各组间肠道菌群的多样性指数（Ace、Chao1、Shannon、Simpson）无明显差异（P > 0.05）；④模型组与空白组菌群分布差异显著（P < 0.01），中药组肠道菌群部分重叠于模型组；⑤空白组中Ruminococcus、Rikenellaceae和其他属高表达，模型组Paludibacteraceae和Verrucomicrobia表现突出，中药组Alphaproteobacteria和Desulfovibrio高表达；⑥空白组肠道菌群呈厌氧和生物膜合成特征，模型组中革兰氏阴性菌增加，可移动基因原件和生物膜合成减少，而中药组则表现出革兰氏阴性和生物膜合成特征的恢复。 结论： 胆囊结石形成过程中肠道菌群结构变化明显，而大黄灵仙方能够有效调节菌群趋向正常稳态。, authors=陈金梅1,2 , 甘苡榕3 , 俞渊1 , 付军1 , 覃毅1 , 唐乾利2 , authorsList=陈金梅, 甘苡榕, 俞渊, 付军, 覃毅, 唐乾利, authorCompany=1 广西中医药大学第一附属医院，广西 南宁 530299;
中医药学报
|实验研究
2026
, 54
(1) :
19
-25
大黄灵仙方对胆囊结石豚鼠模型肠道菌群的影响
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陈金梅1,2 , 甘苡榕3 , 俞渊1 , 付军1 , 覃毅1 , 唐乾利2
作者信息
1 广西中医药大学第一附属医院，广西 南宁 530299;
通讯作者:
* 俞渊（1981-），男，博士，教授，主任医师，博士生导师，从事肝胆疾病的中医防治研究。
作者简介:
陈金梅（1992-），女，博士研究生，住院医师，从事肝胆疾病的中医防治研究。
Effect of Dahuang Lingxian Formula on the Intestinal Microbiota in Guinea Pig Model of Gallstones
CHEN Jinmei1,2 , GAN Yirong3 , YU Yuan1 , FU Jun1 , QIN Yi1 , TANG Qianli2
Affiliations
1 The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530299, China;
doi: 10.19664/j.cnki.1002-2392.260004
文章导航
目的： 探讨大黄灵仙方对胆囊结石豚鼠模型肠道菌群结构与功能的影响。 方法： 50只豚鼠按随机数字表法分为空白组15只、模型组20只、中药组15只。空白组给予豚鼠正常饲料喂养，每日予以0.9%生理盐水灌胃；模型组给予高脂致石饲料喂养，每日予以0.9%生理盐水灌胃；中药组给予高脂致石饲料喂养，每日予以1 g/mL大黄灵仙方灌胃。共干预8周。收集第8周的结肠粪便，利用16SrDNA测序分析肠道菌群的组成、多样性及功能预测。 结果： ①空白组OTUs数为4 623，模型组为3 970，中药组为4 335；3组共有OTUs为474；②主要优势菌门包括Bacteroidetes、Firmicutes、Proteobacteria、Verrucomicrobia和Spirochaetes；模型组中Bacteroidetes和Verrucomicrobia增加，而Firmicutes和Spirochaetes减少，中药组Bacteroidetes、Firmicutes、Verrucomicrobia减少，但Proteobacteria和Spirochaetes增加；③各组间肠道菌群的多样性指数（Ace、Chao1、Shannon、Simpson）无明显差异（P > 0.05）；④模型组与空白组菌群分布差异显著（P < 0.01），中药组肠道菌群部分重叠于模型组；⑤空白组中Ruminococcus、Rikenellaceae和其他属高表达，模型组Paludibacteraceae和Verrucomicrobia表现突出，中药组Alphaproteobacteria和Desulfovibrio高表达；⑥空白组肠道菌群呈厌氧和生物膜合成特征，模型组中革兰氏阴性菌增加，可移动基因原件和生物膜合成减少，而中药组则表现出革兰氏阴性和生物膜合成特征的恢复。 结论： 胆囊结石形成过程中肠道菌群结构变化明显，而大黄灵仙方能够有效调节菌群趋向正常稳态。
大黄灵仙方
/
胆囊结石
/
肠道菌群
/
16SrDNA
Objective: To investigate the impact of Dahuang Lingxian Formula (Rheum Officinale and Radix Clematidis Formula, DHLX) on the intestinal microbiota's structure and function in guinea pigs suffering from gallbladder stones.Methods: Fifty guinea pigs were randomly assigned to a blank group, a model group, and a traditional Chinese medicine(TCM) group. The model group consumed a high - fat diet to induce gallbladder stones, while the TCM group received DHLX for 8 weeks alongside the same high - fat diet. Colon fecal samples were collected at the 8th week and subjected to 16S rDNA sequencing for analyzing intestinal flora composition, diversity, and functional predictions.Results: ①The OTU count was 4623 in the normal group, 3970 in the model group, and 4335 in the TCM group; 474 OTUs were common to all three groups. ② Major dominant phyla encompassed Bacteroidetes, Firmicutes, Proteobacteria, Verrucomicrobia, and Spirochaetes. In the model group, Bacteroidetes and Verrucomicrobia increased, while Firmicutes and Spirochaetes decreased. Conversely, in the TCM group, Bacteroidetes, Firmicutes, and Verrucomicrobia decreased, while Proteobacteria and Spirochaetes increased. ③ The intestinal flora diversity index (Ace, Chao1, Shannon, Simpson) showed no significant difference between groups. ④ The intestinal flora distribution of the model group significantly diverged from the normal group, with partial overlap observed between the TCM and model groups.⑤In the blank group, Ruminococcus, Rikenellaceae, and other genera were highly expressed; Paludibacteraceae and Verrucomicrobia were prominent in the model group; Alphaproteobacteria and Desulfovibrio were highly expressed in the TCM group. ⑥The intestinal microbiota of the blank group exhibited anaerobic and biofilm synthesis traits. In the model group, Gram - negative bacteria increased, while mobile genetic elements and biofilm synthesis decreased. However, in the TCM group, the characteristics of Gram - negative bacteria and biofilm synthesis were restored.Conclusion: The intestinal microbiota structure undergoes significant changes during gallbladder stone formation, and DHLX effectively regulates the flora, restoring it to a normal steady state.
Dahuang Lingxian Formula
/
Gallstones
/
Intestinal microbiota
/
16SrDNA
陈金梅, 甘苡榕, 俞渊, 付军, 覃毅, 唐乾利.
大黄灵仙方对胆囊结石豚鼠模型肠道菌群的影响.
中医药学报,
2026
, 54
(1)
: 19
-25
.
DOI: 10.19664/j.cnki.1002-2392.260004
CHEN Jinmei, GAN Yirong, YU Yuan, FU Jun, QIN Yi, TANG Qianli.
Effect of Dahuang Lingxian Formula on the Intestinal Microbiota in Guinea Pig Model of Gallstones[J].
Acta Chinese Medicine and Pharmacology ,
2026
, 54
(1)
: 19
-25
.
DOI: 10.19664/j.cnki.1002-2392.260004
2026年第54卷第1期
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doi: 10.19664/j.cnki.1002-2392.260004
接收时间：2024-11-15
首发时间：2026-09-09
https://castjournals.cast.org.cn/joweb/zyyxb/CN/10.19664/j.cnki.1002-2392.260004
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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