Article(id=1304438787497550229, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1304438786377674789, articleNumber=null, orderNo=null, doi=10.19664/j.cnki.1002-2392.260003, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1729353600000, receivedDateStr=2024-10-20, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788932042240, onlineDateStr=2026-09-09, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788932042240, onlineIssueDateStr=2026-09-09, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788932042240, creator=13701087609, updateTime=1788932042240, updator=13701087609, issue=Issue{id=1304438786377674789, tenantId=1146029695717560320, journalId=1302318977290174537, year='2026', volume='54', issue='1', pageStart='1', pageEnd='128', issueExtLink='null', onlineDate='null', pubDate='1768838400000', pubDateStr='2026-01-20', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788932041973, creator='13701087609', updateTime=1788934739716, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304450101590651052, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1304438786377674789, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304450101590651053, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1304438786377674789, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=10, endPage=18, ext={EN=ArticleExt(id=1304438787795345815, articleId=1304438787497550229, tenantId=1146029695717560320, journalId=1302318977290174537, language=EN, title=Mechanism of Yangxin Granules in Regulating GSK3β, COX2 and AQP2 to Intervene in Water - Sodium Retention in Heart Failure Rats, columnId=null, journalTitle=Acta Chinese Medicine and Pharmacology, columnName=null, runingTitle=null, highlight=null, articleAbstract=To investigate the mechanism of Yangxin Granules in regulating GSK3β, COX2 and AQP2 to intervene in water - sodium retention in heart failure rats. Methods: One hundred SPF - grade SD rats were randomly divided into sham operation group (10 rats) and modeling group (90 rats). The modeling group underwent ligation of the left anterior descending coronary artery for 2 weeks to establish a post - myocardial infarction heart failure model. The modeling group was then randomly divided into model group, Yangxin Granules low - dose, medium - dose, and high - dose groups, tolvaptan group, and combined intervention group (tolvaptan + Yangxin Granules). All groups received once - daily drug interventions. Urine output was collected at 6, 12, and 24 h during weeks 2 and 4 of treatment. After the final administration, cardiac ultrasound was performed to record left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS). Urine output and body weight were recorded, and heart and kidney indices were calculated. Serum brain natriuretic peptide (BNP) levels were measured by ELISA. Myocardial tissue pathology was observed via HE and Masson staining. Western blot was used to detect expression levels of GSK3β, COX2, and AQP2 proteins in renal tissues.Results: Compared with the model group, Yangxin Granules increased urine output(P < 0.05), decreased serum BNP levels (P <0.05), improved cardiac function (LVEF and LVFS, P < 0.05, P <0.01), and upregulated COX2 expression (P <0.05). Compared with the model group, the tolvaptan and combination groups showed downregulated GSK3β and AQP2 expression (P <0.01). Yangxin Granules alleviated myocardial tissue damage and inflammatory cell infiltration.Conclusion: Yangxin Granules can regulate renal tubular GSK3β and COX2 expression, thereby inhibiting AQP2, reducing water reabsorption, thus exerting therapeutic effects on water - sodium retention in heart failure., authors=WANG Yang1 , ZHANG Junru2 , DAI Xin1 , WANG Cong1 , GAO Xiaolong3 , LIU Ge1 , REN Dezhi2 , authorsList=WANG Yang, ZHANG Junru, DAI Xin, WANG Cong, GAO Xiaolong, LIU Ge, REN Dezhi, authorCompany=1 Shaanxi University of Chinese Medicine, Xianyang 712046, China; 目的： 探究养心颗粒调控GSK3β、COX2和AQP2干预心衰大鼠水钠潴留的作用机制。 方法： SPF级SD大鼠100只，随机分为假手术组（10只）和造模组（90只），将造模组结扎冠状动脉左前降支2周，诱导心肌梗死后心衰大鼠模型，随后将造模组随机分为模型组，养心颗粒低、中、高剂量组，托伐普坦组和联合干预组（托伐普坦联合养心颗粒），各组大鼠接受1次/d的药物干预，在给药第2周和第4周，分别在6、12、24 h的时间点收集尿量。末次给药后取材，通过超声心动图记录各组大鼠的左室射血分数（LVEF）和左室短轴缩短分数（LVFS）。记录大鼠尿量、体质量，计算得出心脏和肾脏指数。ELISA法检测大鼠血清脑钠肽（BNP）水平；HE和Masson染色对大鼠心肌组织进行病理学观察；Western blot法检测大鼠肾脏组织中GSK3β、COX2和AQP2蛋白的表达水平。 结果： 与模型组比较，养心颗粒可以使心衰大鼠尿量增加（P < 0.05，P < 0.01），血清BNP水平下降（P < 0.05），心功能指标LVEF、LVFS值升高（P < 0.05，P < 0.01），并使COX2的表达上调（P < 0.05）。与模型组比较，托伐普坦组及养心颗粒+托伐普坦组GSK3β及AQP2的表达下调（P < 0.01）；养心颗粒能改善心衰大鼠心肌组织损伤及炎性细胞浸润。 结论： 养心颗粒可通过调控肾小管GSK3β、COX2的表达，继而抑制AQP2，减少水的重吸收，发挥改善心力衰竭水钠潴留的作用。, authors=王洋1 , 张军茹2 , 代鑫1 , 王聪1 , 高小龙3 , 刘歌1 , 任得志2 , authorsList=王洋, 张军茹, 代鑫, 王聪, 高小龙, 刘歌, 任得志, authorCompany=1 陕西中医药大学，陕西 咸阳 712046;
中医药学报
|实验研究
2026
, 54
(1) :
10
-18
养心颗粒调控GSK3β、COX2和AQP2干预心衰大鼠水钠潴留的作用机制
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王洋, 张军茹, 代鑫, 王聪, 高小龙, 刘歌, 任得志
作者信息
通讯作者:
* 任得志（1983-），男，博士，副主任医师，研究方向：中西医结合治疗心血管病。
作者简介:
王洋（1999-），男，硕士研究生，研究方向：中西医结合治疗心血管病。
Mechanism of Yangxin Granules in Regulating GSK3β, COX2 and AQP2 to Intervene in Water - Sodium Retention in Heart Failure Rats
WANG Yang, ZHANG Junru, DAI Xin, WANG Cong, GAO Xiaolong, LIU Ge, REN Dezhi
Affiliations
1 Shaanxi University of Chinese Medicine, Xianyang 712046, China;
doi: 10.19664/j.cnki.1002-2392.260003
文章导航
目的： 探究养心颗粒调控GSK3β、COX2和AQP2干预心衰大鼠水钠潴留的作用机制。 方法： SPF级SD大鼠100只，随机分为假手术组（10只）和造模组（90只），将造模组结扎冠状动脉左前降支2周，诱导心肌梗死后心衰大鼠模型，随后将造模组随机分为模型组，养心颗粒低、中、高剂量组，托伐普坦组和联合干预组（托伐普坦联合养心颗粒），各组大鼠接受1次/d的药物干预，在给药第2周和第4周，分别在6、12、24 h的时间点收集尿量。末次给药后取材，通过超声心动图记录各组大鼠的左室射血分数（LVEF）和左室短轴缩短分数（LVFS）。记录大鼠尿量、体质量，计算得出心脏和肾脏指数。ELISA法检测大鼠血清脑钠肽（BNP）水平；HE和Masson染色对大鼠心肌组织进行病理学观察；Western blot法检测大鼠肾脏组织中GSK3β、COX2和AQP2蛋白的表达水平。 结果： 与模型组比较，养心颗粒可以使心衰大鼠尿量增加（P < 0.05，P < 0.01），血清BNP水平下降（P < 0.05），心功能指标LVEF、LVFS值升高（P < 0.05，P < 0.01），并使COX2的表达上调（P < 0.05）。与模型组比较，托伐普坦组及养心颗粒+托伐普坦组GSK3β及AQP2的表达下调（P < 0.01）；养心颗粒能改善心衰大鼠心肌组织损伤及炎性细胞浸润。 结论： 养心颗粒可通过调控肾小管GSK3β、COX2的表达，继而抑制AQP2，减少水的重吸收，发挥改善心力衰竭水钠潴留的作用。
养心颗粒
/
心力衰竭
/
水钠潴留
/
GSK3β
/
COX2
/
AQP2
To investigate the mechanism of Yangxin Granules in regulating GSK3β, COX2 and AQP2 to intervene in water - sodium retention in heart failure rats. Methods: One hundred SPF - grade SD rats were randomly divided into sham operation group (10 rats) and modeling group (90 rats). The modeling group underwent ligation of the left anterior descending coronary artery for 2 weeks to establish a post - myocardial infarction heart failure model. The modeling group was then randomly divided into model group, Yangxin Granules low - dose, medium - dose, and high - dose groups, tolvaptan group, and combined intervention group (tolvaptan + Yangxin Granules). All groups received once - daily drug interventions. Urine output was collected at 6, 12, and 24 h during weeks 2 and 4 of treatment. After the final administration, cardiac ultrasound was performed to record left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS). Urine output and body weight were recorded, and heart and kidney indices were calculated. Serum brain natriuretic peptide (BNP) levels were measured by ELISA. Myocardial tissue pathology was observed via HE and Masson staining. Western blot was used to detect expression levels of GSK3β, COX2, and AQP2 proteins in renal tissues.Results: Compared with the model group, Yangxin Granules increased urine output(P < 0.05), decreased serum BNP levels (P <0.05), improved cardiac function (LVEF and LVFS, P < 0.05, P <0.01), and upregulated COX2 expression (P <0.05). Compared with the model group, the tolvaptan and combination groups showed downregulated GSK3β and AQP2 expression (P <0.01). Yangxin Granules alleviated myocardial tissue damage and inflammatory cell infiltration.Conclusion: Yangxin Granules can regulate renal tubular GSK3β and COX2 expression, thereby inhibiting AQP2, reducing water reabsorption, thus exerting therapeutic effects on water - sodium retention in heart failure.
Yangxin Granules
/
Heart failure
/
Water - sodium retention
/
GSK3β
/
COX2
/
AQP2
王洋, 张军茹, 代鑫, 王聪, 高小龙, 刘歌, 任得志.
养心颗粒调控GSK3β、COX2和AQP2干预心衰大鼠水钠潴留的作用机制.
中医药学报,
2026
, 54
(1)
: 10
-18
.
DOI: 10.19664/j.cnki.1002-2392.260003
WANG Yang, ZHANG Junru, DAI Xin, WANG Cong, GAO Xiaolong, LIU Ge, REN Dezhi.
Mechanism of Yangxin Granules in Regulating GSK3β, COX2 and AQP2 to Intervene in Water - Sodium Retention in Heart Failure Rats[J].
Acta Chinese Medicine and Pharmacology ,
2026
, 54
(1)
: 10
-18
.
DOI: 10.19664/j.cnki.1002-2392.260003
2026年第54卷第1期
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doi: 10.19664/j.cnki.1002-2392.260003
接收时间：2024-10-20
首发时间：2026-09-09
https://castjournals.cast.org.cn/joweb/zyyxb/CN/10.19664/j.cnki.1002-2392.260003
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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