中医药学报

基于蛋白质组学探讨如意金黄散促进放射性皮肤损伤修复的作用及潜在机制

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薛莹 ¹ , 庞浡仚 ¹ , 张怡雯 ¹ , 曲玉婷 ¹ , 康宁 ² , 龚文心 ¹ , 张浩军 ³^,^* , 李仝 ¹^,²^,^*

中医药学报 | 实验研究 2026,54(8): 14-26

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中医药学报 |实验研究 2026 , 54 (8) : 14 -26

基于蛋白质组学探讨如意金黄散促进放射性皮肤损伤修复的作用及潜在机制

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薛莹¹, 庞浡仚¹, 张怡雯¹, 曲玉婷¹, 康宁², 龚文心¹, 张浩军³^,^*, 李仝¹^,²^,^*

作者信息

^1.北京中医药大学，北京 100029

^2.北京中医药大学第三附属医院，北京 100029

^3.中日友好医院临床医学研究所，北京 100029

通讯作者:

^* 张浩军（1977-），男，博士，副研究员，研究方向：中医药防治纤维化的药效评价与作用机制研究。

李仝（1966-），男，博士，教授，主任医师，研究方向：中西医结合防治恶性肿瘤及血液病。

作者简介:

薛莹（1996-），女，博士研究生，研究方向：中西医结合防治恶性肿瘤及血液病。

Exploration on the Role and Potential Mechanisms of Ruyi Jinhuang Powder in Promoting the Repair of Radiation-Induced Skin Injury Based on Proteomics

Ying XUE¹, Boxian PANG¹, Yiwen ZHANG¹, Yuting QU¹, Ning KANG², Wenxin GONG¹, Haojun ZHANG³^,^*, Tong LI¹^,²^,^*

Affiliations

^1.Beijing University of Chinese Medicine, Beijing 100029, China

^2.The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100029, China

^3.Institute of Clinical Medical Sciences, China-Japan Friendship Hospital, Beijing 100029, China

出版时间: 2026-08-20 doi: 10.19664/j.cnki.1002-2392.260155

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摘要

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目的：

探讨如意金黄散（RYJHS）对放射性皮肤损伤大鼠创面修复的促进作用及其潜在机制。

方法：

采用UHPLC-QE-MS对RYJHS化学成分进行非靶标检测。将75只雄性SD大鼠随机分为正常组（Normal）、模型组（Model）、三乙醇胺组（TEA）、RYJHS低频组（RYJHS-L）及RYJHS高频组（RYJHS-H）。除正常组外，其余各组大鼠均在左侧臀部以90 Gy单次照射建立放射性皮肤损伤模型，随后药物连续治疗8周。通过创面拍照及ImageJ测量评估创面愈合情况。采用HE染色观察皮肤组织病理学改变，天狼星红染色、免疫组化评价胶原沉积。于辐照后4周取正常组、模型组及RYJHS高频组皮肤组织进行DIA定量蛋白质组学分析，并对差异蛋白进行功能富集分析。

结果：

UHPLC-QE-MS共获得1 490个可注释化合物，结合外用制剂理化性质筛选得到95个候选成分，为后续机制分析提供了化学物质基础。RYJHS可促进放射性皮肤损伤创面闭合，提高愈合率（P <0.01），减轻表皮结构破坏及真皮炎性细胞浸润（P <0.001），改善胶原沉积不足及纤维排列紊乱（P <0.001，P <0.000 1），其中高频给药组作用更为明显。蛋白质组学共定量9 075个蛋白，其中模型组与正常组比较，共鉴定差异表达蛋白3 183个；RYJHS高频组与模型组比较，共鉴定差异表达蛋白1 614个。进一步分析显示，RYJHS可逆转1 186个异常表达蛋白，其主要包括表皮屏障功能、角化分化、紧密连接及细胞外基质重塑相关分子。GO富集分析显示，RYJHS干预后差异蛋白在生物学过程层面主要富集于中间丝组织、异生物刺激反应、胆固醇生物合成及角化等过程。KEGG富集分析显示，差异蛋白显著富集于肌动蛋白细胞骨架调控、内吞作用、MAPK信号通路、PI3K-Akt信号通路、内质网蛋白加工、泛素介导的蛋白水解、PPAR信号通路、NOD样受体信号通路及代谢通路等。

结论：

RYJHS可促进放射性皮肤损伤创面修复，改善组织学损伤及胶原相关基质重塑异常。蛋白质组学结果提示，其作用可能与表皮屏障重建、细胞结构稳态、炎症应答、信号转导及蛋白质稳态调节等过程有关。上述结果为后续开展关键靶点验证及功能研究提供了实验基础及理论依据。

关键词

放射性皮肤损伤 / 如意金黄散 / 中医外治 / 蛋白质组学 / 基质重塑

Abstract

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Objective:

To investigate the promoting effect of Ruyi Jinhuang Powder on wound repair in rats with radiation-induced skin injury and its potential mechanisms.

Methods:

Untargeted detection of the chemical components of Ruyi Jinhuang Powder was performed using UHPLC-QE-MS. Seventy-five male SD rats were randomly divided into normal group, model group, triethanolamine group (TEA), Ruyi Jinhuang Powder low-frequency group (Ruyi Jinhuang Powder-L), and Ruyi Jinhuang Powder high-frequency group (Ruyi Jinhuang Powder-H). Except for the normal group, rats in all other groups received a single dose of 90 Gy irradiation on the left buttock to establish a radiation-induced skin injury model, followed by continuous drug treatment for 8 weeks. Wound healing was assessed by wound photography and ImageJ measurement. Histopathological changes were observed via HE staining, and collagen deposition was evaluated by Sirius Red staining and immunohistochemistry. Skin tissues from the normal group, model group, and Ruyi Jinhuang Powder-H group were collected at 4 weeks post-irradiation for DIA quantitative proteomic analysis, followed by functional enrichment analysis of differentially expressed proteins.

Results:

A total of 1, 490 annotable compounds were identified by UHPLC-QE-MS, and 95 candidate components were screened based on the physicochemical properties of the topical formulation, providing a chemical basis for subsequent mechanistic analysis. Ruyi Jinhuang Powder promoted wound closure in radiation-induced skin injury, increased the healing rate (P <0.01), alleviated epidermal structural disruption and dermal inflammatory cell infiltration (P <0.001), and improved insufficient collagen deposition and disordered fiber arrangement (P < 0.001, P < 0.000 1), with more pronounced effects in the high-frequency group. Proteomics quantified a total of 9,075 proteins, among which 3,183 differentially expressed proteins were identified between the model group and the normal group, and 1,614 differentially expressed proteins were identified between the Ruyi Jinhuang Powder-H group and the model group. Further analysis revealed that Ruyi Jinhuang Powder reversed 1, 186 abnormally expressed proteins, mainly involving molecules related to epidermal barrier function, keratinization/differentiation, tight junctions, and extracellular matrix remodeling. GO enrichment analysis showed that after Ruyi Jinhuang Powder intervention, the differentially expressed proteins were mainly enriched in biological processes such as intermediate filament organization, response to xenobiotic stimulus, cholesterol biosynthesis, and keratinization. KEGG enrichment analysis showed that the differentially expressed proteins were significantly enriched in pathways including regulation of actin cytoskeleton, endocytosis, MAPK signaling pathway, PI3K-Akt signaling pathway, protein processing in endoplasmic reticulum, ubiquitin-mediated proteolysis, PPAR signaling pathway, NOD-like receptor signaling pathway, and metabolic pathways.

Conclusion:

Ruyi Jinhuang Powder can promote wound repair in radiation-induced skin injury, improve histological damage and collagen-related abnormalities, and its mechanisms may involve the regulation of epidermal barrier function, keratinization/differentiation, tight junctions, extracellular matrix remodeling, and protein homeostasis. These findings provide an experimental basis and theoretical foundation for subsequent validation of key targets and functional studies.

Key words

Radiation-induced skin injury / Ruyi Jinhuang Powder / External treatment of traditional Chinese medicine / Proteomics / Matrix remodeling

引用本文

薛莹, 庞浡仚, 张怡雯, 曲玉婷, 康宁, 龚文心, 张浩军, 李仝. 基于蛋白质组学探讨如意金黄散促进放射性皮肤损伤修复的作用及潜在机制. 中医药学报, 2026 , 54 (8) : 14 -26 . DOI: 10.19664/j.cnki.1002-2392.260155

Ying XUE, Boxian PANG, Yiwen ZHANG, Yuting QU, Ning KANG, Wenxin GONG, Haojun ZHANG, Tong LI. Exploration on the Role and Potential Mechanisms of Ruyi Jinhuang Powder in Promoting the Repair of Radiation-Induced Skin Injury Based on Proteomics[J]. Acta Chinese Medicine and Pharmacology, 2026 , 54 (8) : 14 -26 . DOI: 10.19664/j.cnki.1002-2392.260155

基金

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国家自然科学基金面上项目(81973783)

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2026年第54卷第8期

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文章信息

doi: 10.19664/j.cnki.1002-2392.260155

接收时间：2026-03-24
首发时间：2026-09-17
出版时间：2026-08-20

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出版历史

收稿日期：2026-03-24
修回日期：2026-06-30

基金

国家自然科学基金面上项目(81973783)

作者信息

^1.北京中医药大学，北京 100029

^2.北京中医药大学第三附属医院，北京 100029

^3.中日友好医院临床医学研究所，北京 100029

通讯作者:

^* 张浩军（1977-），男，博士，副研究员，研究方向：中医药防治纤维化的药效评价与作用机制研究。

李仝（1966-），男，博士，教授，主任医师，研究方向：中西医结合防治恶性肿瘤及血液病。

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https://castjournals.cast.org.cn/joweb/zyyxb/CN/10.19664/j.cnki.1002-2392.260155

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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