中医药学报

制远志对肾不藏志不寐大鼠脑组织IL-4、IL-18表达的差异研究

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胡燕玲 ¹ , 张星平 ¹^,^* , 贾宏林 ¹ , 吴金鸿 ¹ , 梁瑞宁 ² , 陈旭 ¹ , 李振辉 ¹ , 李海明 ¹ , 崔文军 ¹ , 左广珂 ¹

中医药学报 | 实验研究 2026,54(5): 7-13

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中医药学报 |实验研究 2026 , 54 (5) : 7 -13

制远志对肾不藏志不寐大鼠脑组织IL-4、IL-18表达的差异研究

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胡燕玲¹, 张星平¹^,^*, 贾宏林¹, 吴金鸿¹, 梁瑞宁², 陈旭¹, 李振辉¹, 李海明¹, 崔文军¹, 左广珂¹

作者信息

^1.新疆医科大学第四临床医学院，新疆乌鲁木齐 830099

^2.新疆医科大学中医学院，新疆乌鲁木齐 830017

通讯作者:

^* 张星平（1961-），男，硕士，教授，主任医师，从事中医神志病诊断及治疗研究。

作者简介:

胡燕玲（1997-），女，硕士研究生，从事中医神志病诊断及治疗研究。

Study on the Differential Expression of IL-4 and IL-18 in the Brain Tissue of Insomnia Model Rats with 'Kidney Failing to Store Willpower' Treated with the Processed Polygala tenuifolia

Yanling HU¹, Xingping ZHANG¹^,^*, Honglin JIA¹, Jinhong WU¹, Ruining LIANG², Xu CHEN¹, Zhenhui LI¹, Haiming LI¹, Wenjun CUI¹, Guangke ZUO¹

Affiliations

^1.The Fourth Clinical College of Xinjiang Medical University, Urumqi 830099, China

^2.College of Traditional Chinese Medicine, Xinjiang Medical University, Urumqi 830017, China

出版时间: 2026-05-20 doi: 10.19664/j.cnki.1002-2392.260089

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摘要

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目的：

探讨制远志对肾不藏志不寐大鼠脑组织中IL-4、IL-18表达水平的影响。

方法：

将40只SD雄性大鼠随机分为正常组（Normal）、模型组（Model）、艾司唑仑组（ESTZ）、制远志低剂量组（PT-LD）、制远志高剂量组（PT-HD），每组8只。采用D-半乳糖（D-gal）联合对氯苯丙氨酸（PCPA）制备肾不藏志不寐大鼠模型，艾司唑仑组予以艾司唑仑0.2 mg·kg^-1·d^-1灌胃，制远志低剂量组予以制远志2.1 g·kg^-1·d^-1灌胃，制远志高剂量组予以制远志4.2 g·kg^-1·d^-1灌胃，正常组和模型组予以等体积生理盐水灌胃。Morris水迷宫实验评价大鼠学习记忆能力，HE和Nissl染色观察大鼠脑组织中细胞形态及结构；IHC和Western blot检测大鼠脑组织中IL-4、IL-18的蛋白表达水平。

结果：

与正常组比较，模型组大鼠潜伏期及运动总距离显著增加（P < 0.01），目标象限探索时间百分比显著降低（P <0.01），脑组织中神经元核偏位，核仁增大，炎性细胞浸润，尼氏体数量显著减少（P < 0.01），神经元细胞胞体间间隙增宽，神经突触结构不完整，IL-4表达水平显著减少（P < 0.01），IL-18表达水平显著增加（P < 0.01）；与模型组比较，制远志低剂量组、制远志高剂量组大鼠潜伏期及运动总距离显著降低（P < 0.01），目标象限探索时间百分比显著增加（P < 0.01），神经元损伤情况及炎性细胞浸润不同程度减轻，尼氏体数量不同程度增加（P < 0.01），神经元和胞体形态结构不同程度改善，IL-4表达水平显著增加（P < 0.01），IL-18表达水平显著降低（P < 0.01）。

结论：

制远志能够显著改善肾不藏志不寐大鼠的睡眠及学习记忆能力，并减轻肾不藏志不寐大鼠的神经元细胞炎症损伤状态，其改善睡眠机制可能与升高脑组织中IL-4、降低IL-18表达水平有关。

关键词

制远志 / 肾不藏志 / 不寐 / 白细胞介素4 / 白细胞介素18

Abstract

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Objective:

To investigate the difference of expression levels of IL-4 and IL-18 in the brain tissue of insomnia model rats with 'kidney failing to store willpower' Treated with the Processed Polygala tenuifolia.

Methods:

A total of 40 male SD rats were randomly divided into normal group, model group, Esazolam group, Processed Polygala tenuifolia low-dose group and Processed Polygala tenuifolia high-dose group with 8 rats in each group. D-galactose (D-gal) combined with p-chlorophenylalanine (PCPA) was used to prepare the rats with 'kidney failing to store willpower'. After successful modeling, the western medicine group was given Esazolam 0.2mg/kg/d by gavage, and the low dose Chinese medicine group was given Processed Polygala tenuifolia 2.1g/kg/d by gavage, the high dose group was given 4.2g/kg/d gavage, and the normal group and model group were given equal volume normal saline gavage for 7 days. Morris water maze test was used to evaluate the learning and memory ability of rats. HE and Nissl staining were used to observe the cell morphology and structure of rat brain. The expression levels of IL-4 and IL-18 in rat brain were detected by IHC and Western blot.

Results:

Compared with normal group, incubation period and total motor distance of rats in model group were significantly increased (P <0.01), the percentage of exploration time in the target quadrant was significantly reduced (P <0.01). The neuronal nucleus was dislocated, the nucleolus was enlarged, the inflammatory cells were infiltrated, and the number of nissl was significantly decreased (P < 0.01). The intercellular spaces of neuron cells are widened, and the synaptic structure is incomplete, and the expression level of IL-4 was significantly decreased (P <0.01), the expression level of IL-18 was significantly increased (P < 0.01). Compared with model group, latency and total distance of movement of rats in low dose group and high dose group were significantly decreased (P < 0.01), the percentage of exploration time in the target quadrant significantly increased (P < 0.01). The neuronal damage and inflammatory cell infiltration were alleviated to varying degrees, and the number of nissl was increased to varying degrees (P < 0.01). The morphology and structure of neuronal nucleosomes were improved to varying degrees, and the expression level of IL-4 was significantly increased (P < 0.01), the expression level of IL-18 was significantly decreased (P <0.01).

Conclusion:

The Processed Polygala tenuifolia can significantly improve the sleep, learning and memory ability of insomnia model rats with 'kidney failing to store willpower', and alleviate the inflammatory damage state of the neurons in rats with 'kidney failing to store willpower'. The mechanism of improving the sleep of the rats with insomnia model rats with 'kidney failing to store willpower' may be related to the increase of IL-4 and the decrease of IL-18 expression level in the brain tissue.

Key words

Processed polygala tenuifolia / Kidney failing to store willpower / Insomnia / IL-4 / IL-18

引用本文

胡燕玲, 张星平, 贾宏林, 吴金鸿, 梁瑞宁, 陈旭, 李振辉, 李海明, 崔文军, 左广珂. 制远志对肾不藏志不寐大鼠脑组织IL-4、IL-18表达的差异研究. 中医药学报, 2026 , 54 (5) : 7 -13 . DOI: 10.19664/j.cnki.1002-2392.260089

Yanling HU, Xingping ZHANG, Honglin JIA, Jinhong WU, Ruining LIANG, Xu CHEN, Zhenhui LI, Haiming LI, Wenjun CUI, Guangke ZUO. Study on the Differential Expression of IL-4 and IL-18 in the Brain Tissue of Insomnia Model Rats with 'Kidney Failing to Store Willpower' Treated with the Processed Polygala tenuifolia[J]. Acta Chinese Medicine and Pharmacology, 2026 , 54 (5) : 7 -13 . DOI: 10.19664/j.cnki.1002-2392.260089

基金

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国家自然科学基金项目(82160873; 81960837; 81560762; 81260526)
新疆自然科学基金重点项目(2022D01D48)
全国名老中医药专家传承工作室(国中医药人教函〔2022〕 75号)
国家中医药管理局高水平中医药重点学科建设项目(国中医药人教函〔2023〕85号)
新疆“十四五”中医学重点学科(新教函〔2022〕112号)

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2026年第54卷第5期

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文章信息

doi: 10.19664/j.cnki.1002-2392.260089

接收时间：2025-05-07
首发时间：2026-09-17
出版时间：2026-05-20

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出版历史

收稿日期：2025-05-07
修回日期：2025-12-04

基金

国家自然科学基金项目(82160873; 81960837; 81560762; 81260526)

新疆自然科学基金重点项目(2022D01D48)

全国名老中医药专家传承工作室(国中医药人教函〔2022〕 75号)

国家中医药管理局高水平中医药重点学科建设项目(国中医药人教函〔2023〕85号)

新疆“十四五”中医学重点学科(新教函〔2022〕112号)

作者信息

^1.新疆医科大学第四临床医学院，新疆乌鲁木齐 830099

^2.新疆医科大学中医学院，新疆乌鲁木齐 830017

通讯作者:

^* 张星平（1961-），男，硕士，教授，主任医师，从事中医神志病诊断及治疗研究。

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https://castjournals.cast.org.cn/joweb/zyyxb/CN/10.19664/j.cnki.1002-2392.260089

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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