Article(id=1307269644465762838, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1307269637637427792, articleNumber=null, orderNo=null, doi=10.19664/j.cnki.1002-2392.260072, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1736265600000, receivedDateStr=2025-01-08, revisedDate=1756656000000, revisedDateStr=2025-09-01, acceptedDate=null, acceptedDateStr=null, onlineDate=1789606971135, onlineDateStr=2026-09-17, pubDate=1776614400000, pubDateStr=2026-04-20, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789606971135, onlineIssueDateStr=2026-09-17, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789606971135, creator=13701087609, updateTime=1789606971135, updator=13701087609, issue=Issue{id=1307269637637427792, tenantId=1146029695717560320, journalId=1302318977290174537, year='2026', volume='54', issue='4', pageStart='1', pageEnd='127', issueExtLink='null', onlineDate='null', pubDate='1776614400000', pubDateStr='2026-04-20', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789606969508, creator='13701087609', updateTime=1789606969508, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=31, endPage=38, ext={EN=ArticleExt(id=1307269644629340695, articleId=1307269644465762838, tenantId=1146029695717560320, journalId=1302318977290174537, language=EN, title=Study on HPLC Fingerprint and Anti-inflammatory Effects of Viola yedoensis, columnId=null, journalTitle=Acta Chinese Medicine and Pharmacology, columnName=null, runingTitle=null, highlight=null, articleAbstract=
Objective: To establish an HPLC fingerprint of Viola yedoensis using HPLC technology, evaluate the quality of 10 batches of Viola yedoensis combined with cluster analysis (HCA) and principal component analysis (PCA), and study its anti-inflammatory mechanism.
Methods: A Waters Symmetry C18 column (250 mm × 4.6 mm, 5 μm) was used with a mobile phase of acetonitrile -0.1% phosphoric acid aqueous solution; flow rate: 0.8 mL·min-1; injection volume: 10 μL; column temperature: 30 ℃; detection wavelength: 353 nm. The anti-inflammatory effect was observed using the ear swelling method, and the levels of PGE2, IL -6, and TNF-α in serum were detected.
Results: The HPLC fingerprint of 10 batches of Viola yedoensis was successfully established, with 10 common peaks identified. The similarities were all higher than 0.990. Two common peaks were identified as cichoriin (2.44 ± 1.11) mg·g-1 and esculetin (10.50 ± 1.57) mg·g-1. The 10 batches of samples were classified into two categories by chemical pattern recognition methods. The aqueous decoction of Viola yedoensis could inhibit ear swelling in mice and reduce the levels of TNF-α, IL-6, and PGE2 in serum.
Conclusion: This study successfully established the HPLC fingerprint of 10 batches of Viola yedoensis and found that its anti-inflammatory mechanism may be related to the inhibition of TNF-α, IL-6, and PGE2 expression, providing a scientific basis and pharmacodynamic support for the quality control and clinical application of Viola yedoensis.
, authors=Hang SUN, Zhenhua ZHANG, Lili HUANG
*, authorsList=Hang SUN, Zhenhua ZHANG, Lili HUANG, authorCompany=null, correspAuthors=Lili HUANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1307269644981662232, articleId=1307269644465762838, tenantId=1146029695717560320, journalId=1302318977290174537, language=CN, title=紫花地丁HPLC指纹图谱及其抗炎作用研究, columnId=1303760928135213534, journalTitle=中医药学报, columnName=中药研究, runingTitle=null, highlight=null, articleAbstract=
目的： 通过HPLC技术建立紫花地丁指纹图谱，结合聚类分析（HCA）、主成分分析（PCA）对10批紫花地丁进行质量评价，研究其抗炎机制。
方法： 采用Waters Symmertry C18色谱柱（250 mm ×4.6 mm, 5 μm），流动相：乙腈-0.1%磷酸水溶液；流速：0.8 mL·min-1；进样量：10 μL；柱温：30 ℃；检测波长：353 nm。耳肿胀法观察抗炎作用，并检测血清中PGE2、IL -6、TNF-α的含量。
结果： 成功建立10批紫花地丁药材的HPLC指纹图谱，确认10个共有峰，相似度均高于0.990，指认菊苣苷（2.44 ± 1.11）mg·g-1和秦皮乙素（10.50 ± 1.57） mg·g-1两个共有峰。通过化学模式识别方法将10批样品分为两大类。紫花地丁水煎液能抑制小鼠耳肿胀，降低血清TNF-α、IL -6及PGE2水平。
结论： 本研究成功建立10批紫花地丁的HPLC指纹图谱，发现其抗炎作用机制可能与抑制TNF-α、IL-6及PGE2的表达相关，为紫花地丁的质量控制和临床应用提供了科学依据和药效学支持。
, authors=孙杭, 张振华, 黄莉莉
*, authorsList=孙杭, 张振华, 黄莉莉, authorCompany=null, correspAuthors=黄莉莉, authorNote=
孙杭（2001-），女，硕士研究生，研究方向：中药及复方改善睡眠药效物质基础及其作用机制。
, correspAuthorsNote=
* 黄莉莉（1978-），女，博士，教授，研究方向：中药及复方改善睡眠药效物质基础及其作用机制。
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孙杭（2001-），女，硕士研究生，研究方向：中药及复方改善睡眠药效物质基础及其作用机制。
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孙杭（2001-），女，硕士研究生，研究方向：中药及复方改善睡眠药效物质基础及其作用机制。
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