Article(id=1303760929020207701, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1303760924272259535, articleNumber=null, orderNo=null, doi=10.19664/j.cnki.1002-2392.260049, pmid=null, cstr=null, oa=null, hot=0, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1735833600000, receivedDateStr=2025-01-03, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1788770428184, onlineDateStr=2026-09-07, pubDate=null, pubDateStr=null, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1788770428184, onlineIssueDateStr=2026-09-07, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1788770428184, creator=13041195026, updateTime=1788770428184, updator=13041195026, issue=Issue{id=1303760924272259535, tenantId=1146029695717560320, journalId=1302318977290174537, year='2026', volume='54', issue='3', pageStart='1', pageEnd='125', issueExtLink='null', onlineDate='null', pubDate='1773936000000', pubDateStr='2026-03-20', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1788770427052, creator='13041195026', updateTime=1788934400812, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1304448680157803505, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1303760924272259535, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1304448680157803506, tenantId=1146029695717560320, journalId=1302318977290174537, issueId=1303760924272259535, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=15, endPage=23, ext={EN=ArticleExt(id=1303760929372529239, articleId=1303760929020207701, tenantId=1146029695717560320, journalId=1302318977290174537, language=EN, title=Effects of Dihuang Yinzi on Improving the Cognitive Function of APP/PSI Double Transgenic Mice by Regulating Synaptic Plasticity via NDRG2, columnId=null, journalTitle=Acta Chinese Medicine and Pharmacology, columnName=null, runingTitle=null, highlight=null, articleAbstract=Objective: To investigate the effects of Dihuang Yinzi on cognitive function of APP/PS1 double transgenic mice and explore its underlying mechanisms.Methods: Forty - five male APP/PS1 double transgenic mice were randomly divided into 5 groups (9 mice per group) : namely the model group (normal saline), the donepezil hydrochloride group(0.84 mg/kg/d), and the low - dose, medium - dose, and high - dose Dihuang Yinzi groups (1.25, 2.5, and 5 g/kg/d, respectively). Additionally, 9 male C57BL/6J mice were used as the blank group (normal saline). All groups were treated continuously for 4 weeks. After 4 weeks, the Morris water maze test was used to evaluate spatial learning and memory abilities in each group. Nissl staining was performed to observe neuronal cells and Nissl bodies in the CA3 region of the hippocampus. Immunohistochemical (I HC) staining was used to observe NDRG2 and GFAP in the CA3 region of the hippocampus. Golgi staining was used to examine the morphology of dendritic spines in pyramidal neurons in the CA3 region of the hippocampus. Immunofluorescence staining was used to detect the expression of postsynaptic density protein - 95 (PSD95) and synaptophysin (SYN) in the CA3 region of the hippocampus. Western blot was used to measure the expression of NDRG2 and EAAT1 in the hippocampus.Results: Compared with the model group, the escape latency of the Morris water maze in each dose group of Dihuang Yinzi was reduced, and the residence time in the target quadrant and the number of crossings in the target quadrant were increased (P <0.01 or P < 0.05). The number of neurons, the number of dendritic branches, and dendritic spines were increased. PSD95 and SYN were up - regulated (P <0.05), while GFAP, NDGR2, and EAAT1 were decreased (P <0.01 or P <0.05).Conclusion: Dihuang Yinzi can improve the cognitive function of APP/PS1 double transgenic mice. Its mechanism of action may be related to down - regulating the expression of NDRG2, regulating astrocytes, subsequently down - regulating EAAT1, reducing the concentration of glutamate in the synaptic cleft, improving synaptic plasticity, and alleviating hippocampal neuronal damage., authors=MANA Lulu1 , LIANG Lili1 , ZHANG Qing2 , YUAN Xiaoxia1 , authorsList=MANA Lulu, LIANG Lili, ZHANG Qing, YUAN Xiaoxia, authorCompany=1 Xinjiang Medical University, Urumqi 830017, China; 目的： 研究地黄饮子对APP/PS1双转基因小鼠认知功能的影响，并探寻其作用机制。方法： 将45只雄性APP/PS1双转基因小鼠随机分为5组，每组9只，即模型组（给予生理盐水）、盐酸多奈哌齐组（给予盐酸多奈哌齐0.84 mg·kg -1 ·d -1 ）、地黄饮子低、中、高剂量组（分别给予地黄饮子1.25、2.5、5 g·kg -1 ·d -1 ）；并将9只雄性C57BL/6J小鼠作为空白组（给予生理盐水），连续干预4周。4周后，采用Morris水迷宫实验评估各组小鼠空间学习记忆能力；尼氏染色观察海马CA3区神经元细胞及尼氏小体；免疫组化（IHC）染色观察海马组织CA3区NDRG2、GFAP；高尔基染色观察海马组织CA3区锥体神经元棘突形态；免疫荧光染色检测海马组织CA3区突触后致密物 - 95（PSD95）、突触素SYN表达；Western blot检测海马NDRG2、EAAT1表达水平。结果： 与模型组比较，地黄饮子各剂量组Morris水迷宫逃避潜伏期减少（P < 0.05，P < 0.01），目标象限停留时间及目标象限穿越次数均增加（P < 0.05，P <0.01），神经元数量、树突分支数及树突棘有所增加，PSD95、SYN上调（P < 0.05，P < 0.01），GFAP、NDGR2、EAAT1有所减少（P < 0.05，P < 0.01）。结论： 地黄饮子可改善APP/PS1双转基因小鼠的认知功能，其作用机制可能与下调NDRG2的表达，调控星形胶质细胞，继而下调EAAT1，降低突触间隙谷氨酸浓度，改善突触可塑性，减轻海马神经元损伤有关。, authors=玛娜璐璐1 , 梁丽丽1 , 张庆2 , 袁晓霞1 , authorsList=玛娜璐璐, 梁丽丽, 张庆, 袁晓霞, authorCompany=1. 新疆医科大学，新疆 乌鲁木齐 830017;
中医药学报
|实验研究
2026
, 54
(3) :
15
-23
地黄饮子通过NDRG2调节突触可塑性改善APP/PSI双转基因小鼠认知功能的作用
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玛娜璐璐1 , 梁丽丽1 , 张庆2 , 袁晓霞1
作者信息
1. 新疆医科大学，新疆 乌鲁木齐 830017;
通讯作者:
* 袁晓霞（1981-），女，博士，副教授，主治医师，研究方向：中医药防治老年性疾病。
作者简介:
玛娜璐璐（1988-），女，博士，副教授，研究方向：神经退行性疾病的中西医结合基础研究。
Effects of Dihuang Yinzi on Improving the Cognitive Function of APP/PSI Double Transgenic Mice by Regulating Synaptic Plasticity via NDRG2
MANA Lulu1 , LIANG Lili1 , ZHANG Qing2 , YUAN Xiaoxia1
Affiliations
1 Xinjiang Medical University, Urumqi 830017, China;
doi: 10.19664/j.cnki.1002-2392.260049
文章导航
目的： 研究地黄饮子对APP/PS1双转基因小鼠认知功能的影响，并探寻其作用机制。方法： 将45只雄性APP/PS1双转基因小鼠随机分为5组，每组9只，即模型组（给予生理盐水）、盐酸多奈哌齐组（给予盐酸多奈哌齐0.84 mg·kg -1 ·d -1 ）、地黄饮子低、中、高剂量组（分别给予地黄饮子1.25、2.5、5 g·kg -1 ·d -1 ）；并将9只雄性C57BL/6J小鼠作为空白组（给予生理盐水），连续干预4周。4周后，采用Morris水迷宫实验评估各组小鼠空间学习记忆能力；尼氏染色观察海马CA3区神经元细胞及尼氏小体；免疫组化（IHC）染色观察海马组织CA3区NDRG2、GFAP；高尔基染色观察海马组织CA3区锥体神经元棘突形态；免疫荧光染色检测海马组织CA3区突触后致密物 - 95（PSD95）、突触素SYN表达；Western blot检测海马NDRG2、EAAT1表达水平。结果： 与模型组比较，地黄饮子各剂量组Morris水迷宫逃避潜伏期减少（P < 0.05，P < 0.01），目标象限停留时间及目标象限穿越次数均增加（P < 0.05，P <0.01），神经元数量、树突分支数及树突棘有所增加，PSD95、SYN上调（P < 0.05，P < 0.01），GFAP、NDGR2、EAAT1有所减少（P < 0.05，P < 0.01）。结论： 地黄饮子可改善APP/PS1双转基因小鼠的认知功能，其作用机制可能与下调NDRG2的表达，调控星形胶质细胞，继而下调EAAT1，降低突触间隙谷氨酸浓度，改善突触可塑性，减轻海马神经元损伤有关。
地黄饮子
/
星形胶质细胞
/
N - myc下游调节基因2
/
突触可塑性
Objective: To investigate the effects of Dihuang Yinzi on cognitive function of APP/PS1 double transgenic mice and explore its underlying mechanisms.Methods: Forty - five male APP/PS1 double transgenic mice were randomly divided into 5 groups (9 mice per group) : namely the model group (normal saline), the donepezil hydrochloride group(0.84 mg/kg/d), and the low - dose, medium - dose, and high - dose Dihuang Yinzi groups (1.25, 2.5, and 5 g/kg/d, respectively). Additionally, 9 male C57BL/6J mice were used as the blank group (normal saline). All groups were treated continuously for 4 weeks. After 4 weeks, the Morris water maze test was used to evaluate spatial learning and memory abilities in each group. Nissl staining was performed to observe neuronal cells and Nissl bodies in the CA3 region of the hippocampus. Immunohistochemical (I HC) staining was used to observe NDRG2 and GFAP in the CA3 region of the hippocampus. Golgi staining was used to examine the morphology of dendritic spines in pyramidal neurons in the CA3 region of the hippocampus. Immunofluorescence staining was used to detect the expression of postsynaptic density protein - 95 (PSD95) and synaptophysin (SYN) in the CA3 region of the hippocampus. Western blot was used to measure the expression of NDRG2 and EAAT1 in the hippocampus.Results: Compared with the model group, the escape latency of the Morris water maze in each dose group of Dihuang Yinzi was reduced, and the residence time in the target quadrant and the number of crossings in the target quadrant were increased (P <0.01 or P < 0.05). The number of neurons, the number of dendritic branches, and dendritic spines were increased. PSD95 and SYN were up - regulated (P <0.05), while GFAP, NDGR2, and EAAT1 were decreased (P <0.01 or P <0.05).Conclusion: Dihuang Yinzi can improve the cognitive function of APP/PS1 double transgenic mice. Its mechanism of action may be related to down - regulating the expression of NDRG2, regulating astrocytes, subsequently down - regulating EAAT1, reducing the concentration of glutamate in the synaptic cleft, improving synaptic plasticity, and alleviating hippocampal neuronal damage.
Dihuang Yinzi
/
Astrocytes
/
N - myc downstream regulatory gene 2
/
Synaptic plasticity
玛娜璐璐, 梁丽丽, 张庆, 袁晓霞.
地黄饮子通过NDRG2调节突触可塑性改善APP/PSI双转基因小鼠认知功能的作用.
中医药学报,
2026
, 54
(3)
: 15
-23
.
DOI: 10.19664/j.cnki.1002-2392.260049
MANA Lulu, LIANG Lili, ZHANG Qing, YUAN Xiaoxia.
Effects of Dihuang Yinzi on Improving the Cognitive Function of APP/PSI Double Transgenic Mice by Regulating Synaptic Plasticity via NDRG2[J].
Acta Chinese Medicine and Pharmacology ,
2026
, 54
(3)
: 15
-23
.
DOI: 10.19664/j.cnki.1002-2392.260049
新疆维吾尔自治区自然科学基金项目 (2021D01C266,2020D01C174)
2026年第54卷第3期
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doi: 10.19664/j.cnki.1002-2392.260049
接收时间：2025-01-03
首发时间：2026-09-07
https://castjournals.cast.org.cn/joweb/zyyxb/CN/10.19664/j.cnki.1002-2392.260049
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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