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tenantId=1146029695717560320, journalId=1315121837170065538, language=CN, title=基于生物信息学探讨重症哮喘气道重塑差异表达基因及中药预测, columnId=null, journalTitle=中医学报, columnName=创新思维与技术前沿, runingTitle=null, highlight=null, articleAbstract=目的:基于生物信息学探讨重症哮喘(severe asthma,SA)气道重塑的关键基因，并映射数据库预测中药，为临床治疗SA提供思路。方法:通过基因表达综合数据库(gene expression omnibus,GEO)获取SA相关数据集，Genecards数据库获取气道重塑基因，鉴定获得SA气道重塑的相关基因，并对相关基因进行功能富集分析。利用机器学习方法进一步筛选SA气道重塑的关键差异表达基因。采用GEO内部数据集和外部数据集进行ROC验证，利用Coremine数据库与获得的关键基因相映射，获得作用于SA气道重塑的潜在中药。结果:本研究获得SA差异基因共53个，其中上调基因29个，下调基因24个;获得12个SA气道重塑相关基因，其中筛选出6个特征基因，共预测中药120味潜在作用于SA气道重塑，以清热药、补虚药和化痰止咳平喘药居多。分子对接显示，特征基因与高频中药的核心成分结合良好。结论:血管内皮生长因子A、原纤维蛋白、CXADR、角蛋白、白细胞介素-1受体2和白细胞介素-18受体1可能是SA气道重塑治疗的潜在生物标志物，推测矮地茶、半枝莲、女贞子、白果等中药可能是SA气道重塑的潜在治疗药物。, authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, 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