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Cloning, Bioinformatics, and Expression Analysis of StMYB3 Gene in Potato
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Hongyu Zhang1, Zaimin Tian1, Wenping Qiu1, Yingshuo Cui1, Lefei Sang1, Yaning Wang1, Shujun Dong1, Yingying Dong1, Shaoying Hou1, Hongyan Han2, Dong Wei3
Crops | 2026, 42(2) : 68 - 73
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Crops | 2026, 42(2): 68-73
Cloning, Bioinformatics, and Expression Analysis of StMYB3 Gene in Potato
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Hongyu Zhang1, Zaimin Tian1, Wenping Qiu1, Yingshuo Cui1, Lefei Sang1, Yaning Wang1, Shujun Dong1, Yingying Dong1, Shaoying Hou1, Hongyan Han2, Dong Wei3
Affiliations
  • 1College of Agriculture and Forestry Science and Technology, Hebei North University, Zhangjiakou 075000, Hebei, China
  • 2Sinochem Modern Agriculture (Inner Mongolia) Co., Ltd., Hohhot 010000, Inner Mongolia, China
  • 3Hebei North University / Hebei Key Laboratory of Agricultural Products and Food Safety Analysis and Testing, Zhangjiakou 075000, Hebei, China
Published: 2026-04-15 doi: 10.16035/j.issn.1001-7283.2026.02.008
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The influence of potato transcription factors on anthocyanin accumulation is primarily reflected in their regulation of genes involved in the anthocyanin biosynthetic pathway. In this study, the StMYB3 gene was cloned by RT-PCR from three potato cultivars: Shepody (white flesh), Wanziqianhong (light purple flesh), and 2013-89-61 (dark purple flesh). Bioinformatics characterization and differential expression analysis were performed. The results showed that the target fragment of StMYB3 genes was 710 bp in length, encoding a protein with a molecular weight of 20.71 kDa and an isoelectric point of 9.14, which was identified as a hydrophilic protein. StMYB3 genes contains R2R3-MYB domain and bHLH interaction motif but lacks a transmembrane domain. Phylogenetic analysis revealed that StMYB3 genes shares the highest sequence similarity with the ScMYB gene from pepper. Semi-quantitative PCR results showed that the expression level of potato StMYB3 gene in different tissues is: tender leaf > old leaf > stem > tuber > root, and the cultivar 2013-89-61 exhibited consistently high expression across all tissues.

Potato  /  Anthocyanins  /  StMYB3  /  Bioinformatics  /  Expression analysis
Hongyu Zhang, Zaimin Tian, Wenping Qiu, Yingshuo Cui, Lefei Sang, Yaning Wang, Shujun Dong, Yingying Dong, Shaoying Hou, Hongyan Han, Dong Wei. Cloning, Bioinformatics, and Expression Analysis of StMYB3 Gene in Potato[J]. Crops, 2026 , 42 (2) : 68 -73 . DOI: 10.16035/j.issn.1001-7283.2026.02.008
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Year 2026 volume 42 Issue 2
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doi: 10.16035/j.issn.1001-7283.2026.02.008
  • Receive Date：2024-11-19
  • Online Date：2026-04-30
  • Published：2026-04-15
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  • Received：2024-11-19
  • Revised：2025-03-10
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Affiliations
    1College of Agriculture and Forestry Science and Technology, Hebei North University, Zhangjiakou 075000, Hebei, China
    2Sinochem Modern Agriculture (Inner Mongolia) Co., Ltd., Hohhot 010000, Inner Mongolia, China
    3Hebei North University / Hebei Key Laboratory of Agricultural Products and Food Safety Analysis and Testing, Zhangjiakou 075000, Hebei, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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