Peanut oil content is a complex trait, which is easily affected by environmental conditions. It is critically important to detect oil content of peanut kernels in peanut breeding. A total of 150 lines of recombinant inbred lines (RIL) derived from the cross between Yuhua 18 (high oil) and AT215 (low oil) were selected as materials, the spectral data of single-kernel was collected by Antaris II near infrared spectrometer, and the oil content was determined. A near-infrared (NIR) detection model on oil content for single-kernel oil content was established by partial least squares (PLS). The Root-Mean-Squares Error of Cross-Validation was 1.04 and the correlation coefficient (R2) was 0.9589. Twenty-four peanut samples with obvious difference in oil content were selected for external validation, and the R2 was 0.9730. Another F2 population from the cross between Yuhua 14 (high oil) and Huayu 20 (low oil) was used as the test population, whose oil content was determined by this model and the present single-kernel model. The variation range of predicted values from these two models was from -0.52% to 1.92%, showing a narrow variation range. This model is relatively small, and is reliable for oil content confirmation, which can be used for the oil content selection and quality detection of peanut.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |