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Outline
Tonoplast intrinsic proteins（TIPs），as important members of the aquaporin family，play crucial roles in plant water balance and stress responses. In this study，the function and molecular mechanism of the PagTIP1；3 gene in response to salt stress were investigated in the leaves of wild-type 84K poplar（Populus alba×P.glandulosa） and PagTIP1；3 knockout lines. The results showed that under normal conditions，knockout of the PagTIP1；3 gene led to decreases in chlorophyll content，net photosynthetic rate，and plant height. Under salt stress，the knockout lines exhibited aggravated wilting and yellowing，significantly reduced plant height，and marked decreases in photosynthetic physiological parameters，including maximum photochemical efficiency，electron transport rate，and relative chlorophyll content，compared with the wild type. Transcriptome analysis revealed that the expression patterns of key genes involved in the photosynthetic pathway（including PagLHCB7，PagGAPCP-2，PagPSY，and PagRCA） were significantly altered in the PagTIP1；3 knockout lines in response to salt stress. In summary，it is speculated that PagTIP1；3 deficiency disrupted the transcriptional response of photosynthesis-related genes in poplar under salt stress，thereby impairing photosynthetic electron transport and photoprotection capacity，disturbing growth and photosynthetic physiological homeostasis，and ultimately significantly reducing salt tolerance in poplar.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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