Full
Outline
Oryza rufipogon Griff.， the direct ancestor of cultivated rice， is a core germplasm resource for rice genetic improvement. At present， the natural habitats of wild rice have suffered severe degradation. Moreover， the in vivo-conserved germplasm in wild rice nurseries is threatened by rice viral diseases， which severely compromised the safe preservation of wild rice resources. In this study， a tissue culture system was constructed， and axillary buds of virus-infected O. rufipogon were used as explants to achieve virus elimination. The results showed that the combination of 5 mg/L 2，4-D and 1 nmol/L OsREF1 could induce callus formation and proliferation from axillary buds， and the combination of 2 mg/L 6-BA and 0.2 mg/L NAA could induce shoot differentiation. The two-step rooting method facilitated root induction and the growth of intact and robust plantlets. RT-PCR showed that the virus-specific bands were absent in the regenerated plantlets. In conclusion， this established system could achieve virus elimination and regeneration of virus-infected O. rufipogon， and provide technical support for the safe preservation of wild rice germplasm resources.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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