Full
Outline
INTRODUCTION A preliminary tissue culture system for Phyllanthus acidus was established.
RATIONALE In this study, the stem tips of P. acidus were used as explants, and the schemes of primary culture, secondary proliferation culture and rooting culture were screened.
RESULTS The results showed that the optimal medium for primary culture was MS+2.0 mg·L-1 6-BA+0.2 mg·L-1 NAA, and the induced germination rate of explants reached 81.11%. The optimal medium for subculture was MS+1.0 mg·L-1 6-BA+0.2 mg·L-1 IBA, and the proliferation coefficient was 1.86. The optimal medium for rooting was MS+1.5 mg·L-1 IBA, and the rooting rate reached 83.00%. After 7 days of cultivation, the plantlets were transplanted with perlite, peat soil and humus=1:1:1 (v/v/v), and the survival rate was 90%.
CONCLUSION The most suitable medium for primary culture using the stem tip of P. acidus as explant was MS medium and the phytohormones were 2.0 mg·L-1 6-BA and 0.2 mg·L-1 NAA; the optimal phytohormones for subculture were 1.0 mg·L-1 6-BA and 0.2 mg·L-1 IBA; the optimal phytohormone for rooting culture was 1.5 mg·L-1 IBA.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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