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To assess the feasibility of farnesyl pyrophosphate synthase (FPPS), a key enzyme in juvenile hormone biosynthesis, as a target for RNA interference (RNAi)-based control of pest mites, double-stranded RNA targeting FPPS (dsFPPS) was designed using the dsRNAEngineer online platform. The bioactivity of dsFPPS against two agriculturally important pest mites, Tetranychus urticae and Tetranychus evansi, was evaluated with microinjection. The expression levels of FPPS in mites after dsFPPS injection were determined by quantitative real-time PCR, and the safety to non-target organisms, Neoseiulus californicus and Harmonia axyridis, was assessed by feeding and microinjection assays. The results showed that after dsFPPS injection, FPPS transcript levels in T. urticae and T. evansi were significantly reduced by 91.80% and 83.09%, respectively. Deutonymphs of both mite species failed to molt normally and died, with mortality rates of 76.97% and 84.32%, respectively. After feeding on or microinjection dsFPPS, N. californicus and H. axyridis developed normally, indicating no significant effects on these natural enemies. These findings demonstrate that dsFPPS has high lethality against pest mites while being safe for non-target organisms. The FPPS gene can therefore serve as an ideal target for RNAi-based control of pest mites and has potential for development as a novel environmentally friendly acaricide.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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