Full
Outline
This study aimed to explore the effects of stratification treatment on breaking the dormancy of Celtis sinensis seeds. The changes in nutrients, endogenous hormones and key enzyme activities during the stratification process were analyzed to clarify its hibernation release mechanism and find the best method for breaking seed dormancy.
Three treatment methods were adopted for Celtis sinensis seeds: variable temperature stratification, low-temperature stratification, and gibberellin combined with low temperature stratification. The physiological indicators such as soluble sugar, soluble protein, starch, crude fat, glucose-6-phosphate dehydrogenase (G-6-PDH), lipase, indole acetic acid (IAA), gibberellin (GA3), zeatin riboside (ZR), and abscisic acid (ABA) of the seeds under different stratification stages were detected to analyze the changing trends of these physiological indicators during the stratification process.
Stratification treatments can significantly increase the germination rate of Celtis sinensis seeds. The effect of gibberellin combined with low temperature stratification treatment is the best, with a germination rate of 54.7% after 100 days of stratification. As the stratification process progresses, the trends of physiological indicators in each treatment are similar: soluble sugar increased first and then decreased, and soluble protein, starch and crude fat were decomposed continuously. The activity of lipase and G-6-PDH enzyme was enhanced continuously. IAA increased first and then decreased, ABA decreased continuously, and the contents of GA3 and ZR increased continuously. The ratios of IAA/ABA, GA3/ABA and ZR/ABA increased continuously. Gibberellin combined with low temperature stratification treatment showed significantly higher enzyme activity and GA3 content in the middle and later stages of stratification compared to the other two treatments, while the ABA content was significantly lower than those of the other two treatments.
Stratification treatment effectively broke the dormancy of Celtis sinensis seeds, and the method of gibberellin combined with low temperature stratification is the best. During the stratification process, the nutrients, enzyme activities and endogenous hormone contents within the seeds are mutually transformed, jointly promoting the transition of Celtis sinensis seeds from dormancy to germination.
14
2
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
