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Based on supramolecular chemistry of TCM, this study investigated the interaction between Cinnamomi Cortex-Coptidis Rhizoma herb pair and compared phase states and bioactivity differences under different decoction methods (co-decoction vs individual decoction followed by mechanical mixing). Phase state variations were observed macroscopically and through turbidimetry. The herb interaction was analyzed using isothermal titration calorimetry, UV and IR spectroscopy. Particle size differences were determined by dynamic light scattering and scanning electron microscopy (SEM). Antimicrobial activity was evaluated through broth dilution, plate coating, live/dead staining, and SEM. Component analysis was conducted using UPLC-MS/MS. Results revealed distinct supramolecular structures in Cinnamomi Cortex-Coptidis Rhizoma decoctions, with co-decoction showing more stable macroscopic phase states. The interaction was a spontaneous exothermic reaction driven by both entropy and enthalpy. UV and IR spectra revealed co-decoction demonstrated more sufficient molecular interactions, more stable supramolecular structures, and smaller particle sizes with a more centralized distribution. In addition, co-decoction resulted in higher berberine and coptisine content and stronger inhibitory activity against Staphylococcus aureus compared to mechanical mixtures. These findings indicated that co-decoction produced Cinnamomi Cortex-Coptidis Rhizoma supramolecular systems with more homogeneous phase states, enhanced measurable coptisine and berberine content, and superior antibacterial efficacy.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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