Article(id=1276976052324925666, tenantId=1146029695717560320, journalId=1276577828834349082, issueId=1276975955985961244, articleNumber=null, orderNo=null, doi=10.19540/j.cnki.cjcmm.20250730.701, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1749657600000, receivedDateStr=2025-06-12, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1782384415942, onlineDateStr=2026-06-25, pubDate=1767196800000, pubDateStr=2026-01-01, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782384415942, onlineIssueDateStr=2026-06-25, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782384415942, creator=13701087609, updateTime=1782384415942, updator=13701087609, issue=Issue{id=1276975955985961244, tenantId=1146029695717560320, journalId=1276577828834349082, year='2026', volume='51', issue='1', pageStart='1', pageEnd='300', issueExtLink='null', onlineDate='null', pubDate='1767196800000', pubDateStr='2026-01-01', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782384392973, creator='13701087609', updateTime=1782384484405, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1276976339546673871, tenantId=1146029695717560320, journalId=1276577828834349082, issueId=1276975955985961244, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1276976339546673872, tenantId=1146029695717560320, journalId=1276577828834349082, issueId=1276975955985961244, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=143, endPage=152, ext={EN=ArticleExt(id=1276976052664664291, articleId=1276976052324925666, tenantId=1146029695717560320, journalId=1276577828834349082, language=EN, title=Asthma-alleviating effects of Poria cocos based on network pharmacology and gut microbiota, columnId=null, journalTitle=Chinese Journal of Traditional Chinese Medicine, columnName=null, runingTitle=null, highlight=null, articleAbstract=

This study investigated the asthma-alleviating effects of Poria cocos from the perspectives of immuno-inflammation and gut microbiota (GM), providing a theoretical basis for its rational clinical use. Male Balb/c mice were randomly divided into normal, model, positive control (montelukast sodium, 1.5 mg·kg^-1), low-dose P. cocos (0.057 g·kg^-1), medium-dose P. cocos (0.114 g·kg^-1), and high-dose P. cocos (0.227 g·kg^-1) group. Except for the normal group, the other groups were sensitized with ovalbumin (OVA) by intraperitoneal injection combined with nebulization to establish an asthma model, and treated with the corresponding drugs. After modeling, cough and wheezing indices, lung function, and asthma characteristic indicators including immunoglobulin E (IgE), interferon-γ (IFN-γ), interleukin 4 (IL4), and interleukin 10 (IL10) levels were measured. Lung tissue pathology was observed by hematoxylin-eosin (HE) and Masson staining. Quantitative real-time PCR (qRT-PCR) was used to detect airway inflammation-and remodeling-related mRNA levels in lung tissue. Levels of 12 inflammatory factors in serum and bronchoalveolar lavage fluid were detected by multiplex assay. Furthermore, the asthma-alleviating mechanism of P. cocos was analyzed by network pharmacology and GM 16S rRNA sequencing. Results showed that P. cocos reduced cough frequency in asthmatic mice, improved lung function, alleviated pathological damage in lung tissue, decreased serum IgE and IL 4 levels, increased IL 10 and IFN-γ levels, and multi-target regulated related inflammatory factors in serum and alveolar lavage fluid, maintaining immune homeostasis. Network pharmacology predicted that P. cocos exerts its asthma-alleviating effects via inflammation regulation pathways. GM sequencing showed that P. cocos effectively alleviated dysbiosis induced by OVA sensitization, significantly downregulated Muribaculum intestinale and Alistipes inops, and correlation analysis revealed both were positively correlated with pro-inflammatory factors. Therefore, it is speculated that P. cocos restores intestinal microecological homeostasis by reducing levels of M. intestinale and A. inops, decreases pro-inflammatory factor production, blocks the inflammatory cascade, and thereby exerts its asthma-alleviating effects.

, authors=null, authorsList=Qi-mei TIE, Meng-nan ZENG, Bei-bei ZHANG, Ru WANG, Chao-yuan XIAO, Ru-yi FAN, Ying-bo HU, Meng-ya WANG, Wei-sheng FENG, Xiao-ke ZHENG, authorCompany=null, correspAuthors=Xiao-ke ZHENG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1276976053172175078, articleId=1276976052324925666, tenantId=1146029695717560320, journalId=1276577828834349082, language=CN, title=基于网络药理学和肠道菌群的茯苓平喘功效探究, columnId=1276975994707775974, journalTitle=中国中药杂志, columnName=药理, runingTitle=null, highlight=null, articleAbstract=

该研究从免疫炎症和肠道菌群（GM）的角度探究茯苓的平喘功效，为临床合理用药提供理论依据。首先将雄性Balb/c小鼠随机分为正常组、模型组、阳性药孟鲁斯特钠组（1.5 mg·kg^-1）、茯苓低剂量组（0.057 g·kg^-1）、茯苓中剂量组（0.114 g·kg^-1）和茯苓高剂量组（0.227 g·kg^-1），除正常组外其余采用卵清蛋白（OVA）腹腔注射联合雾化激发构建哮喘动物模型并给予相应药物，造模结束后检测各组小鼠的咳喘指标、肺功能以及哮喘特征指标免疫球蛋白E（IgE）、干扰素-γ（IFN-γ）、白细胞介素（IL）4和IL10水平；采用苏木精-伊红（HE）染色和马松染色观察肺组织病理变化；qRT-PCR检测小鼠肺组织中气道炎症与重塑相关mRNA水平；多因子检测小鼠血清和肺泡灌洗液中12种炎症因子水平；接着通过网络药理学和GM 16S rRNA测序技术分析茯苓平喘的作用机制。结果显示茯苓可降低哮喘小鼠的咳喘次数，改善肺功能，减轻肺组织病理损伤，降低血清中IgE、IL4水平，升高IL10、IFN-γ水平，多靶点调控哮喘小鼠血清和肺泡灌洗液中相关炎性因子的水平，维持机体的免疫稳态。网络药理学预测茯苓可能通过炎症反应调节通路发挥平喘作用，且GM测序结果显示茯苓可以有效缓解OVA致敏后引起的菌群失调，显著下调肠鼠杆菌Muribaculum intestinale和另枝菌Alistipes inops水平，同时相关性分析结果显示二者与促炎因子正相关。因此推测茯苓可以通过减少肠鼠杆菌和另枝菌水平重建肠道微生态稳态，降低促炎因子的产生，阻断炎症级联反应进而发挥平喘功效。

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