Article(id=1276976026676756573, tenantId=1146029695717560320, journalId=1276577828834349082, issueId=1276975955985961244, articleNumber=null, orderNo=null, doi=10.19540/j.cnki.cjcmm.20250829.501, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1747584000000, receivedDateStr=2025-05-19, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1782384409826, onlineDateStr=2026-06-25, pubDate=1767196800000, pubDateStr=2026-01-01, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782384409826, onlineIssueDateStr=2026-06-25, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782384409826, creator=13701087609, updateTime=1782384409826, updator=13701087609, issue=Issue{id=1276975955985961244, tenantId=1146029695717560320, journalId=1276577828834349082, year='2026', volume='51', issue='1', pageStart='1', pageEnd='300', issueExtLink='null', onlineDate='null', pubDate='1767196800000', pubDateStr='2026-01-01', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782384392973, creator='13701087609', updateTime=1782384484405, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1276976339546673871, tenantId=1146029695717560320, journalId=1276577828834349082, issueId=1276975955985961244, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1276976339546673872, tenantId=1146029695717560320, journalId=1276577828834349082, issueId=1276975955985961244, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=260, endPage=268, ext={EN=ArticleExt(id=1276976027087798368, articleId=1276976026676756573, tenantId=1146029695717560320, journalId=1276577828834349082, language=EN, title=Effects of Danqi Huayu Recipe on retinal cell apoptosis in rat model of light-induced retinal damage, columnId=null, journalTitle=Chinese Journal of Traditional Chinese Medicine, columnName=null, runingTitle=null, highlight=null, articleAbstract=

This study aims to explore the protective mechanism of Danqi Huayu Recipe against light-induced retinal damage (LIRD) in rats from the perspective of cell apoptosis. Sixty male SD rats were randomly allocated into six groups (n=10): normal group, model group, Ziyin Mingmu Pills group (7.8 g·kg^-1), and low-, medium-, and high-dose (3.45, 10.35, and 31.05 g·kg^-1, respectively) Danqi Huayu Recipe groups. A self-made light damage chamber was used for the modeling of LIRD in rats of other groups except normal group. Seven days after modeling, each group received corresponding agents by gavage. Normal group and model group were administrated with an equal volume of distilled water by gavage for 14 d. The retinal function was evaluated by electroretinography. Hematoxylin-eosin (HE) staining was employed to observe the retinal morphology and measure the thickness of the outer nuclear layer (ONL). The cell apoptosis was examined by terminal deoxynucleotidyl transferase dUTP nick end labeling. The ultrastructure of the retina was observed by transmission electron microscopy. The protein and mRNA levels of factors related to retinal cell apoptosis were determined by Western blot and RT-qPCR, respectively. The results showed that compared with normal group, model group demonstrated significantly decreased amplitudes of ERG a-waves and b-waves, thinned ONL, increased cell apoptosis rate, damaged retinal ultrastructure, down-regulated protein and mRNA levels of the anti-apoptotic factor (Bcl-2), and up-regulated protein and mRNA levels of pro-apoptotic factors (Caspase-3, Caspase-6, p53, and Bax). Compared with model group, Ziyin Mingmu Pills group and medium-and high-dose Danqi Huayu Recipe group recovered the amplitudes of ERG a-waves and b-waves, increased the ONL thickness, decreased the cell apoptosis rate, and improved the retinal ultrastructure. Meanwhile, they up-regulated protein and mRNA levels of Bcl-2 and down-regulated protein and mRNA levels of pro-apoptotic factors in a dose-dependent manner. In conclusion, Danqi Huayu Recipe protects rats from LIRD by regulating the expression of proteins and genes related to cell apoptosis to reduce light-induced retinal cell apoptosis.

, authors=null, authorsList=Li HE, Jia-chao YAN, Jing-sheng YU, authorCompany=null, correspAuthors=Jing-sheng YU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1276976027624669282, articleId=1276976026676756573, tenantId=1146029695717560320, journalId=1276577828834349082, language=CN, title=丹芪化瘀方对光诱导的视网膜损伤模型大鼠视网膜细胞凋亡的影响, columnId=1276975994707775974, journalTitle=中国中药杂志, columnName=药理, runingTitle=null, highlight=null, articleAbstract=

该研究旨在从细胞凋亡角度，探究丹芪化瘀方对光诱导的视网膜损伤（LIRD）模型大鼠的保护机制。选取60只雄性SD大鼠，随机分为正常组，模型组，滋阴明目丸组（7.8 g·kg^-1），丹芪化瘀方低（3.45 g·kg^-1）、中（10.35 g·kg^-1）、高（31.05 g·kg^-1）剂量组，每组10只。除正常组外，其余各组利用自制光损伤箱构建LIRD大鼠模型。造模7 d后，各组分别灌胃相应药物，正常组和模型组灌胃等体积蒸馏水，持续14 d。采用视网膜电图（ERG）检测视网膜功能，苏木精-伊红（HE）染色观察视网膜形态并测量外核层（ONL）厚度，TUNEL染色检测细胞凋亡，透射电镜观察视网膜超微结构变化，蛋白免疫印记法（Western blot）与实时荧光定量聚合酶链式反应法（RT-qPCR）分别检测视网膜细胞凋亡相关蛋白及mRNA表达水平。结果显示，与正常组相比，模型组大鼠ERG a波和b波振幅显著降低，视网膜ONL厚度变薄，细胞凋亡率上升，视网膜超微结构受损，抗凋亡蛋白B淋巴细胞瘤-2（Bcl-2）及mRNA表达降低，促凋亡蛋白［胱天蛋白酶（Caspase）-3、Caspase-6、肿瘤抑制蛋白（p53）、Bcl-2相关X蛋白（Bax）］及相关mRNA表达增加；与模型组相比，滋阴明目丸组和丹芪化瘀方中、高剂量组大鼠ERG a波和b波振幅回升，视网膜ONL厚度增加，细胞凋亡率下降，超微结构改善，抗凋亡蛋白（Bcl-2）及mRNA表达上调，促凋亡蛋白（Caspase-3、Caspase-6、p53、Bax）及相关mRNA表达下调，且呈剂量相关性。综上，丹芪化瘀方通过调节细胞凋亡相关蛋白和基因表达，减少光诱导的视网膜细胞凋亡，从而对LIRD模型大鼠发挥保护作用。

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