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To investigate the interventional effects of the Bu-fei Yi-shen Formula(BYF)on airway mitochondrial damage in rats with stable chronic obstructive pulmonary disease (COPD).
Forty rats were randomly assigned to four groups: Control, COPD model, BYF, and Doxofylline (DOX). From week 1 to week 8, stable COPD was induced by twice-daily exposure to cigarette smoke (30 minutes each time) combined with intranasal instillation of Klebsiella pneumoniae (once weekly). From week 9 to week 16, the control and COPD model groups were administered normal saline by gavage at a dose of 2 mL per rat. The BYF and DOX groups received 2 mL of BYF or DOX, respectively, by gavage. In week 16, tissue samples were collected. Subsequently, the degree of mitochondrial damage in lung tissues of COPD rats was evaluated from multiple dimensions, including pulmonary function, histopathology and ultrastructure of lung tissue, mitochondrial membrane potential, activities of mitochondrial respiratory chain complexes, and mRNA and protein expression levels of IP3R, GRP75, and VDAC1.
Compared with the control group, the COPD group showed significantly lower pulmonary function indices(P < 0.05, P < 0.01). Histopathological examination revealed typical COPD-related alterations, including alveolar rarefaction, rupture and fusion of alveolar walls, thickening and corrugation of bronchial walls, accompanied by extensive inflammatory cell infiltration. The mitochondrial membrane potential in lung tissues of the COPD group was significantly reduced, and the activities of respiratory chain complexes II and IV decreased(P < 0.05). Serum levels of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), transforming growth factor- β1(TGF-β1), and interleukin-6(IL-6)were significantly elevated(P < 0.05, P < 0.01). The mRNA and protein expression levels of IP3R, GRP75, and VDAC1 in lung tissues were significantly elevated in the COPD group compared with the control group(P < 0.05, P < 0.01). These pathological alterations were alleviated to varying degrees in the treatment groups, with the BYF group demonstrating greater improvement than the DOX group.
BYF alleviates chronic inflammation and mitochondrial damage in rats with COPD, and its underlying mechanism may be associated with the inhibition of the IP3R/GRP75/VDAC1 signaling pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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