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To investigate the mechanism of Piezo1 activation participating in rat coronary artery smooth muscle cells (CASMCs) calcification under high calcium-phosphate stimulation.
CASMCs were treated with β -glycerophosphate and CaCl2 for 10 days to induce calcification, and cells were divided into the general group(GM)and the calcification group (CM). Then, CASMCs were intervened with inhibitor GsMTx4, siRNA-Piezo1 and agonist Yoda1, respectively. The calcification of CASMCs was examined via alizarin red staining, and the expression of proteins associated with phenotype transition and mitochondrial dynamics was examined via Western blot.
Compared with the GM, the CM had more obvious calcification(P < 0.01). The expression of Piezo1, Runx2, BMP2 and OPN increased(P < 0.01), and the expression of SM22α and SM-MHC decreased(P < 0.01)in the CM. After intervention with GsMTx4 and siRNA-Piezo1, calcification was significantly alleviated(P < 0.01), while intervention with Yoda1 enhanced calcification(P < 0.01). In addition, when stimulated with high calciumphosphorus, there was an increase in the expression of DRP1Ser616 and FIS1(P < 0.01), and a decrease in the expression of MFN1 and MFN2(P < 0.01). Intervention with Yoda1 could aggravate mitochondrial fission (P < 0.01), and intervention with Mdivi-1 could alleviate CASMCs calcification (P < 0.01).
Piezo1 activation can mediate the calcification of CASMCs under high calcium and phosphorus stimulation, and its mechanism may be related to the induction of mitochondrial dynamics imbalance.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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