PDF
Full
Outline
To explore the main active components, targets and pathways of anti-liver fibrosis of Mori fructus by network pharmacology and animal experiment, and to explore its potential mechanism of Mori fructus against liver fibrosis.
The main active components and corresponding targets of Mori fructus were obtained from the traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) and the target information was converted into target gene by Uniprot database. The targets of inflammatory disease were predicted in OMIM database and GeneCards database, and the targets of inflammatory genes were obtained. The network diagram of component-target-pathway was constructed using Cytoscape 3.7.2 software. Protein protein interaction (PPI) network was constructed using the String database and Cytoscape 3.7.2 software, and Gene Ontology (GO) biological process analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were performed. Fifty Kunming mice were randomly divided into six groups, including normal group, model group and low, medium and high dose of total flavonoids from Mori fructus (75,150, 300 mg·kg-1) groups. The liver fibrosis model of mice was induced by carbon tetrachloride, and the serum biochemical indexes and predicted inflammatory indexes were detected. The histopathological changes of liver were observed, and the expression of matrix metalloproteinase 9 (MMP9) protein in liver tissue was detected by immunohistochemical method.
The main anti-inflammation components of Mori fructus include cyanin, quercetin, morin and β-carotene.There were 80 potential anti-inflammatory targets, and the PPI network mainly includes interleukin (IL) -6, tumor necrosis factor (TNF), protein kinase B (AKT) 1, albumin (ALB), vascular endothelial growth factor (VEGFA) and MMP9 and other core targets. The pathways involved mainly include fluid shear stress and atherosclerosis, pathways in cancer, lipid and atherosclerosis, Janus kinase/signal transducer and activator of transcription (JAK-STAT), etc. Animal experimental results showed that compared with the normal group, the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum, the contents of IL-6 and TNF-α in liver tissue as well as the expression of MMP9 protein in the model group were significantly increased (P < 0.01). Compared with the model group, the levels of ALT and AST in serum, the contents of IL-6 and TNF-α and the protein expression of MMP9 protein in liver tissue were decreased (P < 0.05) and the degree of liver injury was alleviated in different doses of total flavonoids from Mori fructus.
Mori fructus has anti-liver fibrosis effects on IL-6, TNF-α, MMP9 and other targets through active components such as anthocyanin and quercetin, which are mainly related to phosphoinositide 3 kinase (PI3K) /AKT, JAK-STAT, and other signaling pathways. Its mechanism of liver protection may be related to the reduction of inflammatory reaction and the down-regulation of the protein expression of MMP9.
PDF
104
53
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
