中国食品学报

降亚硝酸盐菌株短乳杆菌JYX2的全基因组测序及基因功能分析

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任盈 ¹ , 林心萍 ¹ , 董乃慧 ¹ , 李伟 ¹ , 张素芳 ¹ , 郭辉 ¹ , 纪超凡 ¹

中国食品学报 | 分析与检测 2026,26(1): 281-291

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中国食品学报 | 分析与检测 2026, 26(1): 281-291

降亚硝酸盐菌株短乳杆菌JYX2的全基因组测序及基因功能分析

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任盈¹, 林心萍¹, 董乃慧¹, 李伟¹, 张素芳¹, 郭辉¹, 纪超凡¹

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^1.大连工业大学食品学院海洋食品加工与安全控制全国重点实验室国家海洋食品工程技术研究中心海洋食品精深加工关键技术省部共建协同创新中心辽宁大连 116034

Whole Genome Sequencing and Gene Function Analysis of the Nitrite-Lowering Strain Levilactobacillus brevis JYX2

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出版时间: 2026-01-31 doi: 10.16429/j.1009-7848.2026.01.025

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从东北自然发酵酸菜中分离获得1株降亚硝酸盐菌株短乳杆菌JYX2，通过溶血试验与抗生素敏感性试验对其安全性进行评估。为阐明短乳杆菌JYX2的基因组特性及其亚硝酸盐降解相关基因，采用Pacbio三代测序与Illumina二代测序技术进行全基因组测序。基于所得序列，通过COG、GO及KEGG数据库进行基因预测与功能注释分析。利用CARD和VFDB数据库分析短乳杆菌JYX2的安全性。结果显示，短乳杆菌JYX2在含100 μg/mL亚硝酸钠的MRS培养基中培养24 h后，其对培养基中亚硝酸钠的降解效率最高可达98.72%。该菌株未表现出溶血性，而对庆大霉素、红霉素、氯霉素等抗生素均高度敏感(抑制圈直径≥20 mm)，显示出良好的安全性。短乳杆菌JYX2的基因组由1条环状闭合的DNA以及1个环状的质粒组成，基因组长度为2 567 154 bp，GC含量为45.82%，可预测2 485个编码基因，65个tRNA基因，15个rRNA基因以及27个sRNA基因。在COG、GO、KEGG、CAZY、CARD和VFDB数据库中，分别注释到1 926，1 762，1 823，73，180，217个功能基因。信息挖掘分析表明，短乳杆菌JYX2的基因组中未检测到亚硝酸还原酶基因，推测其亚硝酸钠降解作用可能依赖于其它酶或机制。此外，毒力基因与耐药性基因的预测分析进一步证实了短乳杆菌JYX2的安全性。综上，短乳杆菌JYX2具有良好的功能和安全性，可用作发酵食品中亚硝酸盐消减的发酵菌剂。

关键词

短乳杆菌 / 全基因组测序 / 亚硝酸钠 / 基因功能注释 / 酸菜

Abstract

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A nitrite-degrading strain， Levilactobacillus brevis JYX2， was isolated from naturally fermented sauerkraut in Northeast China， and its safety was evaluated through hemolysis test and antibiotic susceptibility test. To elucidate the genomic characteristics of L. brevis JYX2 and its nitrite degradation-related genes， Pacbio third-generation sequencing and Illumina second-generation sequencing technologies were employed for whole-genome sequencing. Based on the obtained sequences， gene prediction and functional annotation analysis were conducted through COG， GO， and KEGG databases. Meanwhile， the safety of L. brevis JYX2 was analyzed using the CARD and VFDB databases. The results showed that L. brevis JYX2 could degrade up to 98.72% of sodium nitrite in the culture medium after 24 h of incubation in MRS medium containing 100 μg/mL sodium nitrite. And the strain exhibited no hemolytic activity and demonstrated high sensitivity to antibiotics such as gentamicin， erythromycin， and chloramphenicol (with inhibition zone diameters ≥ 20 mm)， indicating a favorable safety profile. The genome of L. brevis JYX2 was composed of a circular closed DNA and a circular plasmid， with a genome length of 2 567 154 bp and a GC content of 45.82%. A total of 2 485 coding genes， 65 tRNA genes， 15 rRNA genes， and 27 sRNAs were predicted. Furthermore， 1 926， 1 762， 1 823， 73， 180， 217 functional genes were annotated in COG， GO， KEGG， CAZY， CARD and VFDB databases， respectively. Further analysis revealed that no nitrite reductase gene was detected in the genome of L. brevis JYX2， suggesting that its nitrite degradation may rely on other enzymes or mechanisms. Additionally， predictive analyses of virulence and resistance genes further confirmed the safety of the L. brevis JYX2 strain. In conclusion， L. brevis JYX2 exhibits both functional efficacy and safety， making it a suitable candidate as a fermentation agent for nitrite reduction in fermented foods.

Key words

Levilactobacillus brevis / whole genome sequencing / sodium nitrite / gene function annotation / sauerkraut

引用本文

任盈, 林心萍, 董乃慧, 李伟, 张素芳, 郭辉, 纪超凡. 降亚硝酸盐菌株短乳杆菌JYX2的全基因组测序及基因功能分析. 中国食品学报, 2026 , 26 (1) : 281 -291 . DOI: 10.16429/j.1009-7848.2026.01.025

. Whole Genome Sequencing and Gene Function Analysis of the Nitrite-Lowering Strain Levilactobacillus brevis JYX2[J]. Journal of Chinese Institute of Food Science and Technology, 2026 , 26 (1) : 281 -291 . DOI: 10.16429/j.1009-7848.2026.01.025

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国家自然科学基金面上项目

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2026年第26卷第1期

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doi: 10.16429/j.1009-7848.2026.01.025

接收时间：2024-12-22
首发时间：2026-05-27
出版时间：2026-01-31

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收稿日期：2024-12-22

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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