中国食品学报

基于UHPLC-MS与网络药理学探讨杜仲雄花的抗疲劳机制

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王婷玉 ¹ , 李佳思 ¹ , 时小东 ¹ , 成翠 ¹ , 兰乾敏 ¹

中国食品学报 | 分析与检测 2026,26(1): 268-280

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中国食品学报 | 分析与检测 2026, 26(1): 268-280

基于UHPLC-MS与网络药理学探讨杜仲雄花的抗疲劳机制

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王婷玉¹, 李佳思¹, 时小东¹, 成翠¹, 兰乾敏¹

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^1.成都大学食品与生物工程学院成都 610106

Anti-fatigue of Male Flower of Eucommia ulmoides Based on UHPLC-MS and Network Pharmacology

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出版时间: 2026-01-31 doi: 10.16429/j.1009-7848.2026.01.024

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目的:通过网络药理学和分子对接技术探讨杜仲雄花抗疲劳的作用机制，明确其活性成分与关键靶点间的相互作用，为其应用提供科学依据。方法:采用UPLC-MS分析杜仲雄花活性成分，结合数据库筛选成分靶点与疲劳相关靶点，通过Venn分析确定共同靶点，构建PPI网络及功能通路，采用分子对接验证关键成分与靶点的结合能力。结果:从杜仲雄花中鉴定出63种活性成分及868个作用靶点，筛选出170个交集靶点。网络分析显示，EGFR、AKT1、TP53等为关键靶点，分子对接验证育亨酸一水化合物与关键靶点结合能力强。结论:通过网络药理学和分子对接技术，揭示了杜仲雄花抗疲劳的潜在作用机制。育亨酸一水化合物等活性成分可能通过与EGFR、AKT1、TP53等关键靶点的相互作用，发挥抗疲劳效果。这为杜仲雄花在抗疲劳领域的深入研究和应用提供了理论基础。

关键词

杜仲雄花 / 抗疲劳 / 网络药理学 / 分子对接

Abstract

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Objectives: The purpose of this study was to explore the anti-fatigue mechanism of male flower of Eucommia ulmoides by network pharmacology and molecular docking technology， to clarify the interaction between its active components and key targets， and to provide scientific basis for its application. Methods: UPLC-MS technology was used to analyze the active components of male flower of Eucommia ulmoides， combined with database screening component targets and fatigue related targets， identified common targets by Venn analysis， constructed PPI network and functional pathway analysis， and verified the binding ability of key components and targets by molecular docking. Results: 63 active components and 868 targets were identified and 170 intersection targets were screened. Network analysis showed that EGFR， AKT1 and TP53 were the key targets， and the molecular docking were verified to have strong binding to these targets. Conclusions: This study revealed the potential mechanism of anti-fatigue of male flowers of Euonymus ulmoides by network pharmacology and molecular docking techniques. Active ingredients such as Yohimbic acid monohydrate may exert anti-fatigue effects through interactions with key targets such as EGFR， AKT1， TP53， etc. These findings provide a theoretical basis for further research and application of male flower of Eucommia ulmoides in the field of anti-fatigue.

Key words

male flower of Eucommia ulmoides / anti-fatigue / network pharmacology / molecular docking

引用本文

王婷玉, 李佳思, 时小东, 成翠, 兰乾敏. 基于UHPLC-MS与网络药理学探讨杜仲雄花的抗疲劳机制. 中国食品学报, 2026 , 26 (1) : 268 -280 . DOI: 10.16429/j.1009-7848.2026.01.024

. Anti-fatigue of Male Flower of Eucommia ulmoides Based on UHPLC-MS and Network Pharmacology[J]. Journal of Chinese Institute of Food Science and Technology, 2026 , 26 (1) : 268 -280 . DOI: 10.16429/j.1009-7848.2026.01.024

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四川省自然科学基金项目

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XING M, LIU S, YU Y P, et al.Antibacterial mode of eucommia ulmoides male flower extract against Staphylococcus aureus and its application as a natural preservative in cooked beef[J].Frontiers in Microbiology, 2022, 13: 846622.

ZHAO W B, WANG B Y, LI S.Network pharmacology for traditional Chinese medicine in era of artificial intelligence[J].Chinese Herbal Medicines, 2024, 16(4): 558-560.

WANG J Y, CHEN X J, ZHANG L, et al.Anti-inflammatory effects of Eucommia ulmoides oliv.male flower extract on lipopolysaccharide-induced inflammation[J].Chinese Medical Journal, 2019, 132(3): 319-328.

ZHAI Z, NIU K M, LIU Y, et al.The gut microbiota-bile acids-TGR5 axis mediates Eucommia ulmoides leaf extract alleviation of injury to colonic epithelium integrity[J].Frontiers in Microbiology, 2021, 12: 727681.

步润峰, 张浩然, 龙思雨, 等.基于网络药理学和实验验证探究肉苁蓉治疗阿尔茨海默症作用机制[J/OL].海南医学院学报, 1-15 (2024-12-09) [2025-02-08].https://link.cnki.net/doi/10.13210/j.cnki.jhmu.20241209.002.BU R F, ZHANG H R, LONG S Y, et al.Mechanism of Cistanche deserticola treating Alzheimer's disease based on network pharmacology and experimental validation[J/OL].Journal of Hainan Medical University, 1-15 (2024-12-09) [2025-02-08].https://link.cnki.net/doi/10.13210/j.cnki.jhmu.20241209.002.

DING Z J, LIANG C, WANG X, et al.Antihypertensive activity of Eucommia ulmoides oliv: Male flower extract in spontaneously hypertensive rats[J].Evidence-Based Complementary and Alternative Medicine, 2020, 2020(1): 6432173.

PARK S Y, HAN J S.Neuroprotective effect of bcl-2 on lipopolysaccharide-induced neuroinflammation in cortical neural stem cells[J].International Journal of Molecular Sciences, 2022, 23(12): 6399.

杜庆鑫, 魏艳秀, 刘攀峰, 等.分光光度法测定杜仲雄花和叶中的总黄酮[J].中南林业科技大学学报, 2017, 37(5): 96-100.DU Q X, WEI Y X, LIU P F, et al.Determination on total flavonoids in male flowers and leaves of Eucommia ulmoides by spectrophotometry[J].Journal of Central South University of Forestry & Technology, 2017, 37(5): 96-100.

李英基, 裴水廷, 熊鹏.川赤芝多糖对运动疲劳状态下小鼠心肌组织的保护作用[J].食品工业科技, 2023, 44(6): 366-375.LI Y J, PEI S T, XIONG P.Protective effect of Ganoderma lucidum polysaccharide on myocardial tissue in mice under exercise fatigue[J].Science and Technology of Food Industry, 2023, 44(6): 366-375.

何婵, 罗华丽, 季晖.基于TNF-α的脑缺血再灌注损伤机制及相关药物研究进展[J].中国药房, 2016, 27(28): 4016-4020.HE C, LUO H L, JI H.Research progress on the mechanism of cerebral ischemia-reperfusion injury and related drugs based on TNF-α[J].China Pharmacy, 2016, 27(28): 4016-4020.

LEI Z W, SHI Y J, ZOU J B, et al.A review of the polysaccharides against fatigue and the underlying mechanism[J].International Journal of Biological Macromolecules, 2024, 275(Pt.2): 133601.

LUO C H, XU X R, WEI X C, et al.Natural medicines for the treatment of fatigue: Bioactive components, pharmacology, and mechanisms[J].Pharmacological Research, 2019, 148(1): 104409.

LI Z H, YANG P, XUE S, et al.Testosterone promotion effect of Eucommia ulmoides staminate flower via the steroidogenic pathway and potential hormonal mechanism[J].Scientific Reports, 2022, 12(1): 18765.

李沛慷, 艾连中, 夏永军, 等.杜仲雄花活性成分的提取、活性机制及质量标志物的研究进展[J].工业微生物, 2024, 54(3): 1-3.LI P K, AI L Z, XIA Y J, et al.Research progress on the extraction, activity mechanism and quality markers of active ingredients of Eucommia ulmoides male flowers[J].Industrial Microbiology, 2024, 54(3): 1-3.

刘书伟, 张田田, 王燕, 等.槟榔次碱速效抗疲劳活性及其通过调控血脑屏障发挥抗疲劳机制研究[J].陕西科技大学学报, 2024, 42(5): 67-76.LIU S W, ZHANG T T, WANG Y, et al.Study on the quick acting antifatigue activity of are caidine and its mechanism of anti-fatigue by regulating the blood brain barrier[J].Journal of Shaanxi University of Seience & Technology, 2024, 42(5): 67-76.

李静荣, 梁冰宜, 彭维, 等.基于生物信息学和分子对接技术研究当归六黄汤治疗溃疡性结肠炎潜在活性成分及分子机制[J].药学研究, 2022, 41(9): 561-569.LI J R, LIANG B Y, PENG W, et al.Study on the potential active components and molecular mechanism of Danggui Liuhuang decoction in the treatment of ulcerative colitis based on bioinformatics and molecular docking technology[J].Journal of Pharmaceutical Research, 2022, 41(9): 561-569.

孟益德, 吕庚鑫, 刘攀峰, 等.不同产地杜仲雄花的非靶向代谢组学比较与分析[J].中南林业科技大学学报, 2022, 42(1): 156-166. MENG Y D, L?譈 G X, LIU P F, et al.Comparison and analysis of untargeted metabolomics of male flowers of Eucommia ulmoides from different origins[J].Journal of Central South University of Forestry & Technology, 2022, 42(1): 156-166.

郭洋静, 丁艳霞, 许兰波, 等.HPLC法同时测定杜仲雄花中5种活性成分[J].中成药, 2014, 36(10): 2131-2134.GUO Y J, DING Y X, XU L B, et al.Simultaneous determination of five compounds in male flowers of Eucommia ulmoids oliv by HPLC[J].Chinese Traditional Patent Medicine, 2014, 36(10): 2131-2134.

ZHOU S S, HE C, ZHANG X Q.Anti-fatigue effects of active ingredients from traditional Chinese medicine: A review[J].Current Medicinal Chemistry, 2019, 26(10): 1833-1848.

侯敏, 高文静, 杜阳, 等.降糖消脂片治疗NAFLD的分子作用机制分析[J].中国实验方剂学杂志, 2021, 27(5): 147-157.HOU M, GAO W J, DU Y, et al.Molecular mechanism analysis of Jiangtang Xiaozhi tablets in treatment of NAFLD[J].Chinese Journal of Experimental Traditional Medical Formulae, 2021, 27(5): 147-157.

李丽梅, 于虹敏, 黄美霞, 等.基于网络药理学与实验验证探讨连翘苷治疗脓毒症的作用及机制[J].中药药理与临床, 2024, 40(9): 64-70.LI L M, YU H M, HUANG M X, et al.Effect and mechanism of phillyrin in the treatment of sepsis based on network pharmacology and experimental validation[J].Pharmacology and Clinics of Chinese Materia Medica, 2024, 40(9): 64-70.

赵冬, 金仓.玫瑰花青素提取工艺优化及其抗疲劳研究[J].中国调味品, 2024, 49(10): 205-209, 215.ZHAO D, JIN C.Optimization of extraction process of rose anthocyanins and their anti-fatigue study[J].China Condiment, 2024, 49(10): 205-209, 215.

WU M F, LIU P Y, WANG S Y, et al.Ultrasonic microwave-assisted micelle combined with fungal pretreatment of Eucommia ulmoides leaves significantly improved the extraction efficiency of total flavonoids and gutta-percha[J].Foods, 2021, 10(10): 2399.

DING Y X, DOU D Q, GUO Y J, et al.Simultaneous quantification of eleven bioactive components of male flowers of Eucommia ulmoides oliver by HPLC and their quality evaluation by chemical fingerprint analysis with hierarchical clustering analysis[J].Pharmacognosy Magazine, 2014, 10(40): 435.

ZHENG H, ZHANG W, WANG S, et al.Anti-fatigue effects and mechanism of ginseng combined with caffeine in rats[J].Chinese Journal of Pharmacovigilance, 2024, 21(8): 863.

LI X K, DENG S, LI J R, et al.UPLC-MS analysis and network pharmacology-based investigation into the active ingredients and molecular mechanisms of anti-fatigue of male flowers with Eucommia ulmoides oliv[J].Fundamental & Clinical Pharmacology, 2022, 36(6): 1083-1098.

豆佳红, 王梓颖, 杨娟, 等.基于网络药理学和分子对接分析赤芍抗氧化应激作用的活性成分及其机制[J].中国畜牧兽医, 2023, 50(3): 1229-1240.DOU J H, WANG Z Y, YANG J, et al.Analysis of the aetive ingredients and mechanism of Radix paeoniae Rubra antioxidative stress based on network pharmacology and molecular docking[J].China Animal Husbandry & Veterinary Medicine, 2023, 50(3): 1229-1240.

高耀, 高丽, 高晓霞, 等.基于网络药理学的逍遥散抗抑郁活性成分作用靶点研究[J].药学学报, 2015, 50(12): 1589-1595.GAO Y, GAO L, GAO X X, et al.An exploration in the action targets for antidepressant bioactive components of Xiaoyaosan based on network pharmacology[J].Acta Pharm Sin, 2015, 50(12): 1589-1595.

HUANG L C, LYU Q, ZHENG W Y, et al.Traditional application and modern pharmacological research of Eucommia ulmoides oliv[J].Chinese Medicine, 2021, 16: 73.

范晓旭, 唐琴, 于雪, 等.基于网络药理学和细胞实验探讨和厚朴酚对PC12细胞HIF-1α-VEGF通路的调节作用[J].天然产物研究与开发, 2023, 35(5): 867-878.FANG X X, TANG Q, YU X, et al.Study on regulation of honokiol on HIF-lα-VEGF pathway in PC12 cells based on network pharmacology and experimental verification[J].Nat Prod Res Dev, 2023, 35(5): 867-878.

LI K X, LIU W Z, WU C H, et al.The anti-fatigue and sleep-aiding effects vary significantly among different recipes containing Ganoderma lucidum extracts[J].Heliyon, 2024, 10(10): e32392.

CHEN Q, ZHANG Q, AMROUCHE A T, et al.Asperuloside, the bioactive compound in the edible Eucommia ulmoides male flower, delays muscle aging by daf-16 mediated improvement in mitochondrial dysfunction[J].Food & Function, 2023, 14(12): 5562-5575.

杨萍.杜仲雄花对睾丸间质细胞睾酮分泌的影响及其机制研究[D].武汉: 湖北中医药大学, 2021.YANG P.Effect and mechanism of Eucommia ulmoides male flowers ontestosterone secretion in leydig cells[D].Wuhan: Hubei University of Chinese Medicine, 2021.

WU Y, LIU B W, CAO Y S, et al.Exploring the prescription patterns and molecular mechanism of tonifyingkidney therapy for insomnia using data mining, network pharmacology and molecular docking[J].Modern Chinese Clinical Medicine, 2024, 31(4): 30-37.

邢敏, 费鹏, 史恩聪, 等.响应面法优化杜仲雄花多酚提取工艺及其抗氧化活性[J].食品科技, 2021, 46(7): 201-207, 214.XING M, FEI P, SHI E C, et al.Optimization of extraction and antioxidant activity of polyphenols from Eucommia ulmoides male flower by response surface methodology[J].Food Science and Technology, 2021, 46(7): 201-207, 214.

邹思, 吴岩斌, 吴锦忠, 等.虎奶菇菌核多糖功能化纳米硒抗疲劳功效研究[J].药学实践与服务, 2024, 42(10): 426-432.ZOU S, WU Y B, WU J Z, et al.Anti-fatigue activity of selenium nanoparticles functionalized by polysaccharides from Pleurotus tuber-regium sclerotium[J].Journal of Pharmaceutical Practice and Service, 2024, 42(10): 426-432.

LIU S W, MENG F N, ZHANG D, et al.Lonicera caerulea berry polyphenols extract alleviates exercise fatigue in mice by reducing oxidative stress, inflammation, skeletal muscle cell apoptosis, and by increasing cell proliferation[J].Frontiers in Nutrition, 2022, 9: 853225.

FENG S W, LI T, WEI X R, et al.The antioxidant and anti-fatigue effects of rare ginsenosides and γ-aminobutyric acid in fermented ginseng and germinated brown rice puree[J].International Journal of Molecular Sciences, 2024, 25(19): 10359.

张禄璐, 梁永林, 张李香, 等.基于网络药理学及分子对接探讨旋覆花治疗运动性疲劳的作用机制[J].中国现代医药杂志, 2022, 24(8): 6-12.ZHANG L L, LIANG Y L, ZHANG L X, et al.Mechanism of Inula japonica Thunb in the treatment of exercise-induced fatigue based on network pharmacology and molecular docking[J].China Academic Journal Electronic Publishing House, 2022, 24(8): 6-12.

DING Y X, WU Y T, CHEN J, et al.Protective effect of Eucommia ulmoides oliver male flowers on ethanol-induced DNA damage in mouse cerebellum and cerebral cortex[J].Food Science & Nutrition, 2022, 10(8): 2794-2803.

JAISWAL N, GAVIN M, LORO E, et al.AKT controls protein synthesis and oxidative metabolism via combined mTORC1 and FOXO1 signalling to govern muscle physiology[J].Cachexia Sarcopenia Muscle, 2022, 13(1): 495-514.

ZHANG P, ZHANG D F, ZHOU W A, et al.Network pharmacology: Towards the artificial intelligence-based precision traditional Chinese medicine[J].Briefings in Bioinformatics, 2024, 25(1): 518.

陈丹娜, 吴绮丽.EGFR/HER-1抑制剂通过能量代谢重编程发挥心肌细胞毒性作用[J].循证医学, 2023, 23(6): 371-377.CHEN D N, WU Q L.EGFR/HER-1 inhibitor exert cardiotoxicity through energy metabolic reprogramming[J].The Journal of Evidence-Based Medicine, 2023, 23(6): 371-377.

孙若岚, 梁研, 赵凡, 等.基于网络药理学和实验验证探讨芪术抗癌方治疗结直肠癌的作用机制[J].中医杂志, 2024, 65(4): 404-413.SUN R L, LIANG Y, ZHAO F.Exploring the mechanism of action of Qizhu Kang'ai formula for the treatment of colorectal cancer based on network pharmacology and experimental validation[J].Journal of Traditional Chinese Medicine, 2024, 65(4): 404-413.

2026年第26卷第1期

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doi: 10.16429/j.1009-7848.2026.01.024

接收时间：2025-02-08
首发时间：2026-05-27
出版时间：2026-01-31

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收稿日期：2025-02-08

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四川省自然科学基金项目

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^1.成都大学食品与生物工程学院成都 610106

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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