中国食品学报

骨肽对高原低氧环境下小鼠骨结构的影响

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杨芳 ¹ , 孙胜男 ¹ , 罗唐君 ¹ , 童丽 ¹ , 刘鑫 ²,¹ , 赵成周 ¹

中国食品学报 | 营养与功能 2026,26(1): 132-139

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中国食品学报 | 营养与功能 2026, 26(1): 132-139

骨肽对高原低氧环境下小鼠骨结构的影响

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杨芳¹, 孙胜男¹, 罗唐君¹, 童丽¹, 刘鑫²,¹, 赵成周¹

作者信息

^1.青海大学药学院西宁 810016

^2.金昌市医疗保障局甘肃金昌 737100

Effect of Bone Peptide on Bone Structure in Mice Exposed to High-Altitude Hypoxic Environment

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出版时间: 2026-01-31 doi: 10.16429/j.1009-7848.2026.01.012

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高原低氧环境对人体的影响是生物医学研究的热点之一，特别是对骨骼健康的影响日益受到关注。本研究模拟6 000 m高原低氧环境，建立低氧小鼠模型，探讨骨肽对暴露于该环境下小鼠骨结构的影响。将6~8周龄的C57BL/6N小鼠随机分为空白对照组、模型组、阳性药组和骨肽低、中、高剂量组，每组8只。空白对照组小鼠饲养在常氧环境中，灌胃去离子水；其余各组小鼠置于6 000 m低压氧舱中模拟高原低氧环境，模型组灌胃去离子水，阳性药组和骨肽低、中、高剂量组分别灌胃红景天口服液和不同浓度骨肽溶液，处理14 d。末次给药后，收集血清、脏器及股骨，检测小鼠血清中骨钙素(BGP)和碱性磷酸酶(ALP)含量，并通过Micro-CT扫描评估小鼠股骨的骨结构变化。结果显示:与空白对照组相比，低氧环境下，小鼠的摄食量和体质量均降低，前3 d下降明显，之后逐渐恢复。与模型组相比，骨肽各剂量组均显著提高了小鼠血清中BGP的含量，降低了ALP水平。以2 000 mg/kg/d的骨肽高剂量组效果最好，BGP含量提高了477.18%，ALP含量降低了24.97%。此外，骨肽不同剂量组小鼠骨组织中，骨矿物质含量、骨体积分数、骨小梁厚度和骨小梁数量均提高，而骨小梁分离度降低。表明骨肽可通过提高BGP水平，降低ALP含量，并减少骨小梁分离度，使骨小梁排列紧密，有效维持骨小梁的空间结构，在一定程度上对抗高原低氧环境造成的骨结构损伤，预防骨质疏松的发生。

关键词

骨肽 / 高原低氧 / 骨结构 / 骨钙素 / 碱性磷酸酶

Abstract

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The effects of high-altitude hypoxic environments on the human body are a hot topic in biomedical research， particularly regarding their impact on bone health， which is increasingly gaining attention. This study simulated a high-altitude hypoxic environment at 6 000 meters to establish a hypoxia mouse model， aiming to investigate the influence of bone peptide on the changes in bone structure in mice exposed to this environment. C57BL/6N mice aged 6 to 8 weeks were randomly divided into a blank control group， a model group， a Rhodiola oral solution group， and low， medium， and high-dose bone peptide groups， with 8 mice in each group. The blank control group was raised in a normoxic environment and received deionized water via gavage， while the other groups were placed in a low-pressure oxygen chamber simulating the high-altitude hypoxic environment. The model group received deionized water via gavage， while the positive drug group and the bone peptide low， medium， and high-dose groups were administered Rhodiola oral solution and bone peptide solutions respectively， for a treatment duration of 14 days. After the final administration， serum， organs， and femurs were collected from the mice. The levels of bone gamma-carboxyglutamate protein (BGP) and alkaline phosphatase (ALP) in the serum were measured， and the Micro-CT scans were used to assess changes in bone structure in the femurs of the mice. The results showed that， compared with the blank control group in normoxic conditions， the food intake and body weight of mice in the hypoxic environment decreased， with a significant drop observed in the first three days， followed by a gradual recovery. Compared to the model group， all doses of bone peptide significantly increased the serum levels of BGP and decreased the levels of ALP. The group receiving the high dose of bone peptide (2 000 mg/kg/d) showed the best results， with BGP levels increasing by 477.18% and ALP levels decreasing by 24.97%. Furthermore， the bone tissues of mice in the different bone peptide dosage groups showed increased contents of bone mineral， bone volume fraction， trabecular thickness， and trabecular number， while the trabecular separation decreased. These findings indicate that bone peptide can counteract the bone structural damage induced by high-altitude hypoxic environments and help prevent osteoporosis by increasing BGP levels， reducing ALP levels， and decreasing trabecular separation， thus maintaining the spatial structure of trabecular bone.

Key words

bone peptide / high-altitude hypoxia / bone structure / bone gla protein / alkaline phosphatase

引用本文

杨芳, 孙胜男, 罗唐君, 童丽, 刘鑫, 赵成周. 骨肽对高原低氧环境下小鼠骨结构的影响. 中国食品学报, 2026 , 26 (1) : 132 -139 . DOI: 10.16429/j.1009-7848.2026.01.012

. Effect of Bone Peptide on Bone Structure in Mice Exposed to High-Altitude Hypoxic Environment[J]. Journal of Chinese Institute of Food Science and Technology, 2026 , 26 (1) : 132 -139 . DOI: 10.16429/j.1009-7848.2026.01.012

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青海省“帅才科学家负责制”项目

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2026年第26卷第1期

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文章信息

doi: 10.16429/j.1009-7848.2026.01.012

接收时间：2024-12-26
首发时间：2026-05-27
出版时间：2026-01-31

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收稿日期：2024-12-26

基金

青海省“帅才科学家负责制”项目

作者信息

^1.青海大学药学院西宁 810016

^2.金昌市医疗保障局甘肃金昌 737100

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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