中国食品学报

多光谱法探究金雀异黄酮、柚皮素对胰脂肪酶的抑制机制

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王昆山 ¹ , 于增辉 ¹ , 翟迎奥 ¹ , 周素珍 ¹ , 范金波 ¹

中国食品学报 | 基础研究 2026,26(1): 109-120

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中国食品学报 | 基础研究 2026, 26(1): 109-120

多光谱法探究金雀异黄酮、柚皮素对胰脂肪酶的抑制机制

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王昆山¹, 于增辉¹, 翟迎奥¹, 周素珍¹, 范金波¹

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^1.渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁锦州 121013

Exploring the Inhibition Mechanism of Genistein and Naringenin on Pancreatic Lipase Using Multispectral Methods

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出版时间: 2026-01-31 doi: 10.16429/j.1009-7848.2026.01.010

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探究小分子对胰脂肪酶(PL)的抑制作用，已成为体外筛选具有抗肥胖作用活性成分的重要方法。通过荧光光谱法、非辐射能量转移理论及高效液相色谱法研究金雀异黄酮(Gen)和柚皮素(Nar)与PL的结合反应机制，利用三维荧光、表面疏水性、X射线衍射、红外光谱、粒径、差示扫描量热法、扫描电镜对复合物进行结构表征，阐明它们对PL结构与性质的影响。结果显示，Gen、Nar对PL猝灭均为静态猝灭，结合常数分别为42.39 L/mg和22.57 L/mg，两者的加入使PL的α-螺旋含量降低，β-转角、β-折叠以及无规则卷曲含量增加，结晶度从25.60%分别上升到32.77%，38.00%，表面疏水性降低，热稳定性降低(变性温度从76.04 ℃降低至65.00 ℃和65.05 ℃)，且PL由平滑的片状结构转变为棒状颗粒物。上述结果表明Gen和Nar显著影响PL的结构和性质，为开发新型PL抑制剂提供了新思路。

关键词

多光谱法 / 金雀异黄酮 / 柚皮素 / 胰脂肪酶 / 抑制机制

Abstract

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Exploring the inhibitory effect of small molecules on pancreatic lipase (PL) has become an important method for screening active ingredients with anti-obesity effect in vitro. The binding reaction and mechanism of genistein (Gen) and naringenin (Nar) with PL were studied by fluorescence spectroscopy， non-radiative energy transfer theory and high performance liquid chromatography. At the same time， three-dimensional fluorescence， surface hydrophobicity， X-ray diffraction， infrared spectroscopy， particle size， differential scanning calorimetry， and scanning electron microscopy were used to characterize the structure of the composites from different angles， and their influence on the structure and properties of PL were comprehensively clarified. The results showed that the quenching of PL by Gen and Nar was static quenching， and the binding constants were 42.39 L/mg and 22.57 L/mg， respectively. The addition of both reduced the α-helix content of PL， increased the content of β-turn， β-sheet and random coil， and increased the crystallinity from 25.60% to 32.77% and 38.00%， respectively. The surface hydrophobicity decreased and the thermal stability decreased (the denaturation temperature decreased from 76.04 ℃ to 65.00 ℃ and 65.05 ℃， respectively)， and the PL changed from a smooth sheet structure to a rod-like particle. These results indicate that Gen and Nar significantly affect the structure and properties of PL， providing new ideas for the development of new PL inhibitors.

Key words

multispectral methods / genistein / naringenin / pancreatic lipase / inhibition mechanism

引用本文

王昆山, 于增辉, 翟迎奥, 周素珍, 范金波. 多光谱法探究金雀异黄酮、柚皮素对胰脂肪酶的抑制机制. 中国食品学报, 2026 , 26 (1) : 109 -120 . DOI: 10.16429/j.1009-7848.2026.01.010

. Exploring the Inhibition Mechanism of Genistein and Naringenin on Pancreatic Lipase Using Multispectral Methods[J]. Journal of Chinese Institute of Food Science and Technology, 2026 , 26 (1) : 109 -120 . DOI: 10.16429/j.1009-7848.2026.01.010

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国家重点研发计划项目

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2026年第26卷第1期

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doi: 10.16429/j.1009-7848.2026.01.010

接收时间：2025-02-07
首发时间：2026-05-27
出版时间：2026-01-31

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收稿日期：2025-02-07

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^1.渤海大学食品科学与工程学院辽宁省食品安全重点实验室生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁锦州 121013

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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