中国食品学报

LFcin(17-30)对金黄色葡萄球菌及其生物膜的抑制作用

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于书凝 ¹,²,³ , 耿子健 ¹,²,³ , 温路遥 ¹,²,³ , 李明航 ¹,²,³ , 武瑞 ¹,²,³ , 王建发 ¹,²,³ , 王舰辉 ¹,²,³ , 连帅 ¹,²,³

中国食品学报 | 基础研究 2026,26(1): 37-46

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中国食品学报 | 基础研究 2026, 26(1): 37-46

LFcin(17-30)对金黄色葡萄球菌及其生物膜的抑制作用

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于书凝¹,²,³, 耿子健¹,²,³, 温路遥¹,²,³, 李明航¹,²,³, 武瑞¹,²,³, 王建发¹,²,³, 王舰辉¹,²,³, 连帅¹,²,³

作者信息

^1.黑龙江八一农垦大学动物科技学院黑龙江大庆 163319

^2.农业农村部门东北寒区牛病防治重点实验室黑龙江大庆 163319

^3.黑龙江省牛病防制重点实验室黑龙江大庆 163319

Inhibitory Effect of LFcin (17-30) on Staphylococcus aureus and Its Biofilm

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出版时间: 2026-01-31 doi: 10.16429/j.1009-7848.2026.01.004

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研究抗菌肽LFcin(17-30)对耐甲氧西林金葡菌(MRSA)生物膜的抑制作用。方法:采用PCR及头孢西丁药敏纸片扩散法筛选24株乳源金黄色葡萄球菌中的MRSA；采用刚果红平板法和结晶紫染色法鉴定生物被膜阳性菌株，并评估其产膜能力及生物膜生长曲线；采用纸片扩散法药敏试验验证MRSA生物膜与耐药性间的关系；采用微量稀释法测定LFcin(17-30)对MRSA的最小抑菌浓度(MIC)；采用结晶紫染色和扫描电子显微镜(SEM)测定LFcin(17-30)对MRSA生物膜的抑制作用；测定LFcin(17-30)对MRSA生物膜成分的影响。结果显示:在24株乳源金黄色葡萄球菌中，筛选出4株MRSA，分别为DQ05、DQ07、DQ09、DQ23，生物膜定性均为阳性菌株。结晶紫染色结果显示4株MRSA中DQ05的产膜能力最强，DQ09的产膜能力最弱，且DQ05的耐药性强于DQ09。生物膜生长曲线测定结果表明，在24 h时生物膜量最高。LFcin(17-30)对MRSA的MIC为150 μmol/L。LFcin(17-30)浓度为75 μmol/L时，即可明显抑制生物形成，破坏形成的生物膜。LFcin(17-30)抑制胞外聚合物中胞外多糖和胞外蛋白质的合成和分泌，对胞外DNA的影响随LFcin(17-30)浓度的升高而增强。结论:LFcin(17-30)可广谱抑制食源MRSA流行株及其生物膜形成，有望用于食源MRSA的辅助治疗。

关键词

LFcin(17-30) / 抗菌肽 / MRSA / 生物膜

Abstract

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The inhibitory effect of antimicrobial peptide LFcin(17-30) on methicillin-resistant Staphylococcus aureus (MRSA) biofilm was studied. Methods: MRSA in 24 strains of Staphylococcus aureus were screened by PCR and cefoxitin disk diffusion method. The biofilm-positive strains were identified by Congo red plate method and crystal violet staining method， and the biofilm-forming ability and biofilm growth curve of biofilm-positive strains were evaluated. The relationship between MRSA biofilm and drug resistance was verified by disk diffusion method. The minimal inhibitory concentration(MIC) of LFcin(17-30) against MRSA was determined by microdilution method. The inhibitory effect of LFcin (17-30) on MRSA biofilm was determined by crystal violet staining and scanning electron microscopy (SEM). The effect of LFcin (17-30) biofilm composition was determined. The results showed that 4 strains of MRSA were screened out from 24 strains of Staphylococcus aureus， which were DQ05， DQ07， DQ09 and DQ23， and the biofilm was identified as biofilm positive strains. The results of crystal violet staining showed that DQ05 had the strongest membrane production ability among the four MRSA strains， DQ09 had the weakest membrane production ability， and the drug resistance of DQ05 was stronger than that of DQ09. The biofilm growth curve showed that the amount of biofilm was the highest at 24 h. The MIC of LFcin (17-30) against MRSA was 150 μmol/L. LFcin (17-30) at 75 μmol/L can significantly inhibit the formation and destruction of biofilms. LFcin (17-30) inhibited the synthesis and secretion of extracellular polysaccharides and extracellular proteins in extracellular polymers， and the effect on extracellular DNA increased with the increase of LFcin (17-30) concentration. In summary， LFcin (17-30) can inhibit the prevalence of food-borne MRSA strains and biofilm formation， and is expected to be used as an adjuvant therapy for food-borne MRSA.

Key words

LFcin (17-30) / antimicrobial peptides / methicillin-resistant Staphylococcus aureus / biofilm

引用本文

于书凝, 耿子健, 温路遥, 李明航, 武瑞, 王建发, 王舰辉, 连帅. LFcin(17-30)对金黄色葡萄球菌及其生物膜的抑制作用. 中国食品学报, 2026 , 26 (1) : 37 -46 . DOI: 10.16429/j.1009-7848.2026.01.004

. Inhibitory Effect of LFcin (17-30) on Staphylococcus aureus and Its Biofilm[J]. Journal of Chinese Institute of Food Science and Technology, 2026 , 26 (1) : 37 -46 . DOI: 10.16429/j.1009-7848.2026.01.004

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国家自然科学基金面上项目
黑龙江省“揭榜挂帅”科技攻关项目

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2026年第26卷第1期

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文章信息

doi: 10.16429/j.1009-7848.2026.01.004

接收时间：2025-01-24
首发时间：2026-05-27
出版时间：2026-01-31

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收稿日期：2025-01-24

基金

国家自然科学基金面上项目

黑龙江省“揭榜挂帅”科技攻关项目

作者信息

^1.黑龙江八一农垦大学动物科技学院黑龙江大庆 163319

^2.农业农村部门东北寒区牛病防治重点实验室黑龙江大庆 163319

^3.黑龙江省牛病防制重点实验室黑龙江大庆 163319

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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