中国食品学报

炼乳中耐高渗酵母菌的筛选、鉴定及转录组学分析

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陈唐超 ¹ , 施川川 ² , 肖功年 ¹ , 方若思 ¹

中国食品学报 | 分析与检测 2026,26(1): 329-337

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中国食品学报 | 分析与检测 2026, 26(1): 329-337

炼乳中耐高渗酵母菌的筛选、鉴定及转录组学分析

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陈唐超¹, 施川川², 肖功年¹, 方若思¹

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^1.浙江科技大学生物与化学工程学院杭州 310023

^2.熊猫乳品集团股份有限公司浙江温州 325400

Screening， Identification and Transcriptomic Analysis of Hyperosmotic-tolerant Yeast in Sweetened Condensed Milk

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出版时间: 2026-01-31 doi: 10.16429/j.1009-7848.2026.01.029

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目的:研究高糖胁迫条件下近平滑假丝酵母差异基因的表达水平及其应激响应机制。方法:以贮藏过程中的染菌炼乳为对象，通过平板筛选及18s DNA测序对其中的酵母进行分离、鉴定及特性研究，并提取菌株的总RNA，构建cDNA文库。采用转录组测序，并通过实时荧光定量PCR验证分析差异表达基因。结果:该酵母为近平滑假丝酵母，其最适温度为35 ℃，最适pH值为8，当葡萄糖含量为50%时，仍可以缓慢生长。发酵液中的主要产物为山梨糖醇，产量可达41.06 g/L。通过转录组学分析共检测到2 066个显著性差异基因，其中显著上调993个，显著下调1 073个。与热激蛋白(Hsp70、Hsp90)、氨基酸转运、糖原转运、糖酵解和乙醇脱氢酶相关的基因显著下调，而与氨基酸透过酶、糖原合成以及某些具有渗透压保护作用的氨基酸合成(如谷氨酸、脯氨酸、精氨酸、半胱氨酸等)相关的基因显著上调。结论:假丝酵母通过合成某些糖类或氨基酸(如脯氨酸、甘氨酸和谷氨酸等)来影响应激，提高逆境胁迫的性能。

关键词

炼乳 / 近平滑假丝酵母 / 分离和鉴定 / 转录组学分析

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Objective: To study the expression levels of differentially expressed genes in Candida parapsilosis under high glucose stress and to investigate its stress response mechanism. Methods: The yeast presented in the contaminated condensed milk during storage were isolated and characterized by plate screening and 18s DNA sequencing， and the total RNA of the strains was extracted to construct cDNA libraries. Differentially expressed genes were analyzed by transcriptome analysis and verified by real-time fluorescence quantitative PCR. Results: The yeast was identified as Candida parapsilosis， its optimal temperature and pH value were 35 ℃ and 8， respectively. It could still grow slowly when the glucose concentration reached 50%. The main product in the fermentation broth was sorbitol， with a yield of up to 41.06 g/L. A total of 2 066 significant differential genes were detected in the transcriptomic analysis， of which 993 were significantly up-regulated and 1 073 were significantly down-regulated. The genes related to heat stress proteins (Hsp70， Hsp90)， amino acid transport， glycogen transport， glycolysis and ethanol dehydrogenase were significantly down-regulated， while the genes related to amino acid permease， glycogen synthesis， and synthesis of certain amino acids (e.g.， glutamate， proline， arginine， cysteine， etc.) with osmotic stress-protecting effects were significantly up-regulated. Conclusion: Candida parapsilosis could improve performance under hyperosmotic stress by synthesizing certain sugars or amino acids (e.g.， proline， glycine， and glutamate).

Key words

sweetened condensed milk / Candida parapsilosis / isolation and identification / transcriptomic analysis

引用本文

陈唐超, 施川川, 肖功年, 方若思. 炼乳中耐高渗酵母菌的筛选、鉴定及转录组学分析. 中国食品学报, 2026 , 26 (1) : 329 -337 . DOI: 10.16429/j.1009-7848.2026.01.029

. Screening， Identification and Transcriptomic Analysis of Hyperosmotic-tolerant Yeast in Sweetened Condensed Milk[J]. Journal of Chinese Institute of Food Science and Technology, 2026 , 26 (1) : 329 -337 . DOI: 10.16429/j.1009-7848.2026.01.029

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温州市山区五县揭榜挂帅项目

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2026年第26卷第1期

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doi: 10.16429/j.1009-7848.2026.01.029

接收时间：2025-01-05
首发时间：2026-05-26
出版时间：2026-01-31

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收稿日期：2025-01-05

基金

温州市山区五县揭榜挂帅项目

作者信息

^1.浙江科技大学生物与化学工程学院杭州 310023

^2.熊猫乳品集团股份有限公司浙江温州 325400

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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