中国食品学报

坛紫菜R-藻红蛋白的提取纯化及草甘膦检测应用

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吴夏泠 ¹ , 赵逸凡 ¹ , 李蒙 ¹ , 黄海钊 ¹ , 叶庆琳 ¹ , 张玉苍 ¹

中国食品学报 | 分析与检测 2026,26(1): 305-314

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中国食品学报 | 分析与检测 2026, 26(1): 305-314

坛紫菜R-藻红蛋白的提取纯化及草甘膦检测应用

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吴夏泠¹, 赵逸凡¹, 李蒙¹, 黄海钊¹, 叶庆琳¹, 张玉苍¹

作者信息

^1.集美大学海洋食品与生物工程学院福建厦门 361021

The R-Phycoerythrin Extracted and Purified from Porphyra haitanensis and the Application of Detecting Glyphosate

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出版时间: 2026-01-31 doi: 10.16429/j.1009-7848.2026.01.027

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目的:草甘膦是一种常见的除草剂，常残留于水体中，对环境和人体产生危害，开发高效且准确的检测方法，测定水体中的草甘膦含量有重要意义。本研究基于坛紫菜中的R-藻红蛋白设计一种荧光开启型探针，用于水中草甘膦的检测。方法:通过冻融循环和超声破碎提取坛紫菜中的藻红蛋白并进行纯化。肽指纹图谱、吸收光谱和SDS-PAGE测定结果表明，该产物为R-藻红蛋白。本研究方法获得的R-藻红蛋白纯度高达4.96，达到分析纯级。进一步研究发现，提取的R-藻红蛋白对Cu2+离子表现出优异的特异性荧光响应性。基于该特性设计一种基于铜离子猝灭R-藻红蛋白荧光的探针，利用草甘膦与铜离子螯合的特性实现对草甘膦的检测。结果:该探针的荧光信号与草甘膦浓度呈现良好的线性关系，其最低响应值为443.4 μg/L，远低于国标《生活饮用水卫生标准》(GB 5749—2022)中草甘膦残留物质量浓度的最大限度(0.7 mg/L)。将该探针用于2种实际水样中草甘膦含量的加样回收检验，检出不同含量的草甘膦。结论:本研究为高纯度R-藻红蛋白提取提供了新思路，为检测水体中草甘膦残留提供了一种新方法，具有一定的科学意义和应用价值。

关键词

坛紫菜 / R-藻红蛋白 / 铜离子 / 草甘膦 / 加标回收

Abstract

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Objectives: Glyphosate was an efficient herbicide， while there was negative impact on water environment and human health. Therefore， it is significant to develop an efficient and accurate method to detect the glyphosate in water. In this study， a fluorescent turn-on probe was designed based on R-phycoerythrin in Porphyra haitanensis for detection of glyphosate in water. Methods: The crude extract in P. haitanensis was extracted by freeze-thaw cycle and ultrasonication. The peptide mass fingerprint， abosorbance spectrum and SDS-PAGE indicated the extract was R-phycoerythrin. The purity of R-phycoerythrin was as high as 4.96 after purification. Further studies indicated the R-phycoerythrin was specific fluorescence responsed to Cu2+ ions. A fluorescent probe based on the copper ion quenching R-phycoerythrin fluorescence was designed. Results: The probe fluorescence signal showed good linear relationship with the glyphosate concentration， and the detection limit was 443.4 μg/L， which was significantly lower than the maximum allowable residue limit (0.7 mg/L) specified for glyphosate in the Chinese National Standard for Drinking Water Quality (GB 5749—2022). The probe was used to detect the glyphosate content in two water samples and the glyphosate was tested. Conclusions: The study not only highly-value utilized of P. haitanensis， but also provides a new method for the detection of glyphosate residues in water， which has certain scientific significance and application value.

Key words

Porphyra haitanensis / R-phycoerythrin / copper ion / glyphosate / spike recovery test

引用本文

吴夏泠, 赵逸凡, 李蒙, 黄海钊, 叶庆琳, 张玉苍. 坛紫菜R-藻红蛋白的提取纯化及草甘膦检测应用. 中国食品学报, 2026 , 26 (1) : 305 -314 . DOI: 10.16429/j.1009-7848.2026.01.027

. The R-Phycoerythrin Extracted and Purified from Porphyra haitanensis and the Application of Detecting Glyphosate[J]. Journal of Chinese Institute of Food Science and Technology, 2026 , 26 (1) : 305 -314 . DOI: 10.16429/j.1009-7848.2026.01.027

基金

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国家重点研发计划重点专项
福建省自然科学基金项目
厦门市自然科学基金项目

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2026年第26卷第1期

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doi: 10.16429/j.1009-7848.2026.01.027

接收时间：2025-01-21
首发时间：2026-05-25
出版时间：2026-01-31

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收稿日期：2025-01-21

基金

国家重点研发计划重点专项

福建省自然科学基金项目

厦门市自然科学基金项目

作者信息

^1.集美大学海洋食品与生物工程学院福建厦门 361021

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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