Schistura scaturigina, endemic to the lower reaches of the Yarlung Zangbo River, holds considerable ecological and economic value. In this study, we presented the first systematic report on its embryonic and early larval development under artificial breeding conditions to clarify its developmental chronology and characteristics. These findings fill a critical research gap and provide important implications for the conservation and sustainable utilization of this species. We conducted experiments in April 2024. Hormonal induction was performed using a mixture of luteinizing hormone-releasing hormone analog (LHRH-A₂), human chorionic gonadotropin (HCG), and domperidone (DOM). Females received a two-injection protocol: the first injection consisted of LHRH-A₂ (3 μg/kg)+DOM (2 mg/kg), followed 12 h later by a second injection of LHRH-A₂ (10 μg/kg)+DOM (8 mg/kg)+ HCG (2000 IU/kg). Males received a single injection at the time of the female's second injection, at half the female dosage. Approximately 2,000 fertilized eggs were obtained via artificial dry fertilization, yielding average fertilization and hatching rates of 95.60% and 90.40%, respectively. Over 1,700 healthy larvae were ultimately obtained. The results showed that mature eggs were spherical, light yellow, and lustrous, with slight adhesiveness and a tendency to sink upon contact with water. Egg diameter measured 1.08±0.03 mm, expanding to (2.34± 0.08) mm after water absorption. At a water temperature of (20.0±1.0) ℃, fertilized eggs completed intra-membrane hatching in 49 h 20 min, requiring an accumulated temperature of 986.65 h∙℃. Embryonic development was divided into 33 phases across seven stages: fertilized egg, cleavage, blastula, gastrula, neurula, organogenesis, and hatching. Newly hatched larvae measured (4.18±0.30) mm in total length, exhibiting transparent bodies and no pigmentation on the eyes or skin. Distinct pigmentation appeared across the body at 2 days post-hatching. The yolk sac was nearly absorbed at 5 days post-hatching, coinciding with the development of an oval, vacuole-like, and membranous swim bladder. At 10 days post-hatching, a pair of small spherical bony swim bladders had formed, exhibiting a dumbbell-like shape. Functional organs were essentially fully developed at 15 days post-hatching. Overall growth and development of early larvae demonstrated an increasing trend, albeit with stage-specific variations in developmental rate. Our findings reveal that while embryonic developmental chronology of S. scaturigina aligns with that of phylogenetically close species, specific traits observed in its organogenesis progression and newly hatched larval morphology may reflect adaptations to the unique torrential environment of the lower Yarlung Zangbo River. Given its current restricted distribution, enhanced management within protected areas is recommended. Future efforts should prioritize systematic resource surveys and long-term monitoring to provide a scientific foundation for basin-wide conservation strategies.