Article(id=1292126390019715309, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1292126355785802497, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2026.04.012, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1769616000000, receivedDateStr=2026-01-29, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785996537927, onlineDateStr=2026-08-06, pubDate=1772208000000, pubDateStr=2026-02-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785996537927, onlineIssueDateStr=2026-08-06, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785996537927, creator=13701087609, updateTime=1785996537927, updator=13701087609, issue=Issue{id=1292126355785802497, tenantId=1146029695717560320, journalId=1246415772164075586, year='2026', volume='42', issue='4', pageStart='451', pageEnd='600', issueExtLink='null', onlineDate='null', pubDate='1772208000000', pubDateStr='2026-02-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785996529766, creator='13701087609', updateTime=1786014519902, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1292201811952292026, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1292126355785802497, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1292201811952292027, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1292126355785802497, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=530, endPage=534, ext={EN=ArticleExt(id=1292126390460117230, articleId=1292126390019715309, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Determination of pazopanib concentration in human serum by UPLC, columnId=1246531413395263850, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Research Method, runingTitle=null, highlight=null, articleAbstract=
Objective To establish an ultra performance liquid chromatography (UPLC) method for determining the concentration of pazopanib in human serum.
Methods Carbamazepine was used as an internal standard (IS), and acetonitrile as a protein precipitant. Separation was performed on an Agilent InfinityLab Poroshell 120 EC-C18 column (2.1 x 50 mm, 1.9 μm) with a mobile phase of 0.1% aqueous formic acid-acetonitrile solution in gradient elution mode. The flow rate was 0.25 mL·min-1, detection wavelength was 268 nm, column temperature was 40 ℃, and injection volume was 25 μL. The specificity of the method, the standard curve and the lower limit of quantification, the accuracy and precision and the stability of the sample were investigated.
Results Under the established chromatographic conditions, the analyte and internal standard exhibited satisfactory separation without interference from endogenous substances. Pazopanib demonstrated good linearity within the concentration range of 0.5-100 μg·mL-1. The standard curve was y=63.37×10-2x-0.49×10-2 (r2=0.999 9) and the lower limit of quantification can reach 0.25 μg·mL-1. The relative standard deviations (RSD) for both intra-day. and inter-day precision were less than 12.02%, with extraction recovery rates exceeding 86.44%. Stability was good, with RSD below than 8.80%.
Conclusion The established UPLC method is simple, selective, sensitive, accurate, and reliable for determining pazopanib in human serum.
, authors=Fa-shuang LI, Lin-bo LI, Hui-ying LI, Shao-chun YU, Yan LEI, Hong-min LU, Yi RU, Li-lin ZHANG, Ping LU, Jie KONG, Jin ZHOU, authorsList=Fa-shuang LI, Lin-bo LI, Hui-ying LI, Shao-chun YU, Yan LEI, Hong-min LU, Yi RU, Li-lin ZHANG, Ping LU, Jie KONG, Jin ZHOU, authorCompany=null, correspAuthors=Hui-ying LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1292126391265423599, articleId=1292126390019715309, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=用UPLC法测定人血清中帕唑帕尼浓度, columnId=1246531413600784769, journalTitle=中国临床药理学杂志, columnName=研究方法, runingTitle=null, highlight=null, articleAbstract=
目的 建立超高效液相色谱法（UPLC）测定人血清中帕唑帕尼浓度。
方法 以卡马西平为内标（IS），乙腈为蛋白沉淀剂。采用Agilent InfinityLab Poroshell 120 EC-C18（2.1 x 50 mm，1.9 μm）色谱柱进行分离，流动相为0.1%甲酸水溶液－乙腈溶液，梯度洗脱方式，流速为0.25 mL·min-1，波长为268 nm，柱温为40 ℃，进样量为25μL。考察该方法的专属性、标准曲线、定量下限、精密度、回收率和稳定性。
结果 在该色谱条件下，待测物及内标可良好分离，无其他内源性物质峰干扰。帕唑帕尼在0.25～100 μg·mL-1线性关系良好，标准曲线为y=63.37×10-2x-0.49×10-2（r2=0.999 9）。定量下限为0.25 μg·mL-1。日内、日间精密度的相对标准偏差（RSD）均小于12.02%，提取回收率高于86.44%。稳定性良好，RSD均小于8.80%。
结论 本研究所建立的UPLC法操作简洁，选择性强，灵敏度高，结果准确可靠，适用于人血清中帕唑帕尼的测定。
, authors=李发双, 黎林波, 李惠英, 余绍春, 雷嫣, 卢红敏, 茹毅, 张力麟, 鲁萍, 孔洁, 周瑾, authorsList=李发双, 黎林波, 李惠英, 余绍春, 雷嫣, 卢红敏, 茹毅, 张力麟, 鲁萍, 孔洁, 周瑾, authorCompany=null, correspAuthors=李惠英, authorNote=
李发双（1990-），男，主管药师，主要从事治疗药物监测的工作和研究
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