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Article(id=1291707245839020866, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291706966187991876, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.24.012, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1761926400000, receivedDateStr=2025-11-01, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785896606168, onlineDateStr=2026-08-05, pubDate=1766851200000, pubDateStr=2025-12-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785896606168, onlineIssueDateStr=2026-08-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785896606168, creator=13701087609, updateTime=1785896606168, updator=13701087609, issue=Issue{id=1291706966187991876, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='24', pageStart='3451', pageEnd='3600', issueExtLink='null', onlineDate='null', pubDate='1766851200000', pubDateStr='2025-12-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785896539494, creator='13701087609', updateTime=1785998798662, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1292135872292479273, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291706966187991876, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1292135872296673578, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291706966187991876, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=3524, endPage=3529, ext={EN=ArticleExt(id=1291707246078096195, articleId=1291707245839020866, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Rapid detection of cotinine in urines by colloidal gold immunochromatography, columnId=1246531413395263850, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Research Method, runingTitle=null, highlight=null, articleAbstract=
Objective

To establish a colloidal gold immunochromatographic method for the rapid detection of cotinine in urines.

Methods

To prepare the colloidal gold-labeled antibody probe, optimization of the pH value and the amount of antibody labeling (COT-Mab) was carried out. Following this, the coating concentrations of the cotinine antibody (COT-BSA) for the test (T) line and the sheep anti-mouse immunoglobulin G (IgG) for the control (C) line were also optimized. Subsequently, a colloidal gold immunochromatographic strip was assembled. Finally, the sensitivity of the strip was evaluated.

Results

The optimal conditions established for the colloidal gold immunochromatographic strip were as followings, pH value of 6.5, COT-Mab concentration of 10.00 μg·mL-1, COT-BSA concentration of 0.10 mg·mL-1, and sheep anti-mouse IgG concentration of 1.00 mg·mL-1. The visual detection limit and the cut-off value were 0.01 and 0.20 μg·mL-1, respectively. Within 15 minutes, this method enabled qualitative analysis of cotinine in urines.

Conclusion

The method has good specificity and accuracy, as well as convenient operation and excellent reproducibility, which can be used for on-site detection and rapid initial screening of smokers and non-smokers during drug clinical trials.

, authors=Jun-min DONG1, 4, Long LIU2, 4, Xiao-hua HAO1, 4, Man ZHANG3, 4, 5, authorsList=Jun-min DONG, Long LIU, Xiao-hua HAO, Man ZHANG, authorCompany=null, correspAuthors=Man ZHANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291707246354920260, articleId=1291707245839020866, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=胶体金免疫层析法快速检测尿液中可替宁, columnId=1246531413600784769, journalTitle=中国临床药理学杂志, columnName=研究方法, runingTitle=null, highlight=null, articleAbstract=
目的

建立尿液中可替宁的胶体金免疫层析快速检测方法。

方法

制备金标抗体探针，对pH值和抗体标记量（COT-Mab）进行优化。然后优化检测（T）线可替宁抗体（COT-BSA）的包被浓度、对照（C）线羊抗鼠免疫球蛋白（IgG）的包被浓度，组装胶体金免疫层析试纸，最后评价试纸条的敏感性。

结果

本研究建立的胶体金免疫层析试纸条最优条件是：pH值为6.5、COT-Mab浓度为10.00 μg·mL-1、COT-BSA浓度为0.10 mg·mL-1、羊抗鼠IgG浓度为1.00 mg·mL-1。其视觉检测限和截断值分别为0.01和0.20 μg·mL-1。在15分钟内，该试纸条能够对尿液中的可替宁进行定性分析。

结论

该方法具有良好的特异性和准确性，操作方便，重现性极佳。可用于药物临床试验期间吸烟者和非吸烟者的现场检测和快速的初步筛查。

, authors=董俊敏1, 4, 刘龙2, 4, 郝晓花1, 4, 张曼3, 4, 5, authorsList=董俊敏, 刘龙, 郝晓花, 张曼, authorCompany=null, correspAuthors=张曼, authorNote=

董俊敏（1994-），女，助理研究员，主要从事药物临床试验与质量管理工作

, correspAuthorsNote=
张曼，主任医师，教授 Tel: （010）63926389 E-mail:
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胶体金免疫层析法快速检测尿液中可替宁
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董俊敏 1, 4 , 刘龙 2, 4 , 郝晓花 1, 4 , 张曼 3, 4, 5
中国临床药理学杂志 | 研究方法 2025,41(24): 3524-3529
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中国临床药理学杂志 |研究方法 2025 , 41 (24) : 3524 -3529
胶体金免疫层析法快速检测尿液中可替宁
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董俊敏（1994-），女，助理研究员，主要从事药物临床试验与质量管理工作

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董俊敏（1994-），女，助理研究员，主要从事药物临床试验与质量管理工作

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董俊敏1, 4, 刘龙2, 4, 郝晓花1, 4, 张曼3, 4, 5
作者信息
  • 1.首都医科大学 附属北京世纪坛医院 药物Ⅰ期临床试验研究室，北京 100038
  • 2.首都医科大学 附属北京世纪坛医院 体检中心，北京 100038
  • 3.首都医科大学 附属北京世纪坛医院临床检验医学中心，北京 100038
  • 4.北京大学 第九临床医学院，北京 100038
  • 5.北京市泌尿细胞分子诊断重点实验室，北京 100038
通讯作者:
张曼，主任医师，教授 Tel: （010）63926389 E-mail:
作者简介:

董俊敏（1994-），女，助理研究员，主要从事药物临床试验与质量管理工作

Rapid detection of cotinine in urines by colloidal gold immunochromatography
Jun-min DONG1, 4, Long LIU2, 4, Xiao-hua HAO1, 4, Man ZHANG3, 4, 5
Affiliations
  • 1.Phase Ⅰ Clinical Trial Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • 2.Medical Examination Center, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • 3.Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
  • 4.Peking University Ninth School of Clinical Medicine, Beijing 100038, China
  • 5.Beijing Key Laboratory of Urinary Cellular Molecular Diagnostics, Beijing 100038, China
出版时间: 2025-12-28 doi: 10.13699/j.cnki.1001-6821.2025.24.012
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摘要
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目的

建立尿液中可替宁的胶体金免疫层析快速检测方法。

方法

制备金标抗体探针，对pH值和抗体标记量（COT-Mab）进行优化。然后优化检测（T）线可替宁抗体（COT-BSA）的包被浓度、对照（C）线羊抗鼠免疫球蛋白（IgG）的包被浓度，组装胶体金免疫层析试纸，最后评价试纸条的敏感性。

结果

本研究建立的胶体金免疫层析试纸条最优条件是：pH值为6.5、COT-Mab浓度为10.00 μg·mL-1、COT-BSA浓度为0.10 mg·mL-1、羊抗鼠IgG浓度为1.00 mg·mL-1。其视觉检测限和截断值分别为0.01和0.20 μg·mL-1。在15分钟内，该试纸条能够对尿液中的可替宁进行定性分析。

结论

该方法具有良好的特异性和准确性，操作方便，重现性极佳。可用于药物临床试验期间吸烟者和非吸烟者的现场检测和快速的初步筛查。

可替宁  /  胶体金免疫层析法  /  尿液  /  定性检测
Objective

To establish a colloidal gold immunochromatographic method for the rapid detection of cotinine in urines.

Methods

To prepare the colloidal gold-labeled antibody probe, optimization of the pH value and the amount of antibody labeling (COT-Mab) was carried out. Following this, the coating concentrations of the cotinine antibody (COT-BSA) for the test (T) line and the sheep anti-mouse immunoglobulin G (IgG) for the control (C) line were also optimized. Subsequently, a colloidal gold immunochromatographic strip was assembled. Finally, the sensitivity of the strip was evaluated.

Results

The optimal conditions established for the colloidal gold immunochromatographic strip were as followings, pH value of 6.5, COT-Mab concentration of 10.00 μg·mL-1, COT-BSA concentration of 0.10 mg·mL-1, and sheep anti-mouse IgG concentration of 1.00 mg·mL-1. The visual detection limit and the cut-off value were 0.01 and 0.20 μg·mL-1, respectively. Within 15 minutes, this method enabled qualitative analysis of cotinine in urines.

Conclusion

The method has good specificity and accuracy, as well as convenient operation and excellent reproducibility, which can be used for on-site detection and rapid initial screening of smokers and non-smokers during drug clinical trials.

cotinine  /  colloidal gold immunochromatographic assay  /  urine  /  qualitative detection
董俊敏, 刘龙, 郝晓花, 张曼. 胶体金免疫层析法快速检测尿液中可替宁. 中国临床药理学杂志, 2025 , 41 (24) : 3524 -3529 . DOI: 10.13699/j.cnki.1001-6821.2025.24.012
Jun-min DONG, Long LIU, Xiao-hua HAO, Man ZHANG. Rapid detection of cotinine in urines by colloidal gold immunochromatography[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (24) : 3524 -3529 . DOI: 10.13699/j.cnki.1001-6821.2025.24.012
基金
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  • 首都医科大学附属北京世纪坛医院青年基金资助项目(2023-q07)
  • 首都医科大学附属北京世纪坛医院实验室开放课题专项基金资助项目(2020-KF16)
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文章信息
doi: 10.13699/j.cnki.1001-6821.2025.24.012
  • 接收时间：2025-11-01
  • 首发时间：2026-08-05
  • 出版时间：2025-12-28
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  • 收稿日期：2025-11-01
基金
首都医科大学附属北京世纪坛医院青年基金资助项目(2023-q07)
首都医科大学附属北京世纪坛医院实验室开放课题专项基金资助项目(2020-KF16)
作者信息
    1.首都医科大学 附属北京世纪坛医院 药物Ⅰ期临床试验研究室，北京 100038
    2.首都医科大学 附属北京世纪坛医院 体检中心，北京 100038
    3.首都医科大学 附属北京世纪坛医院临床检验医学中心，北京 100038
    4.北京大学 第九临床医学院，北京 100038
    5.北京市泌尿细胞分子诊断重点实验室，北京 100038

通讯作者:

张曼，主任医师，教授 Tel: （010）63926389 E-mail:
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https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.24.012
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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