Article(id=1291707023847088395, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291706987964822180, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.21.007, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1754582400000, receivedDateStr=2025-08-08, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785896553239, onlineDateStr=2026-08-05, pubDate=1763308800000, pubDateStr=2025-11-17, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785896553239, onlineIssueDateStr=2026-08-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785896553239, creator=13701087609, updateTime=1785896553239, updator=13701087609, issue=Issue{id=1291706987964822180, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='21', pageStart='3001', pageEnd='3150', issueExtLink='null', onlineDate='null', pubDate='1763308800000', pubDateStr='2025-11-17', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785896544684, creator='13701087609', updateTime=1785896721343, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1291707729324826977, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291706987964822180, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1291707729324826978, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291706987964822180, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=3038, endPage=3042, ext={EN=ArticleExt(id=1291707024065192204, articleId=1291707023847088395, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Epidemiological landscape of Enterococcus faecium co-harboring both vanA and vanM resistance genes, columnId=1246531407326105792, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Clinical and Basic Bridging Research, runingTitle=null, highlight=null, articleAbstract=
Objective

To analyze the drug resistance phenotypes and genotypes of 2 307 Enterococcus faecium strains collected from 2011 to 2024.

Methods

The antimicrobial susceptibility testing of the strains was performed using the broth microdilution method, followed by genomic DNA extraction from the strains and polymerase chain reaction (PCR) amplification for screening of resistance genes.

Results

From 2011 to 2012, the detection rate of vancomycin-resistant Enterococcus faecium was 1.67%, and the detection rate of vanM gene-positive vancomycin-susceptible strains was 0.34%. In contrast, from 2023 to 2024, the detection rate of vancomycin-resistant Enterococcus faecium increased to 5.11%, while the detection rate of vanM gene-positive vancomycin-susceptible strains reached as high as 4.75%. Additionally, 22 Enterococcus faecium strains co-harboring both vanA and vanM resistance genes were identified during the period from 2021 to 2024.

Conclusion

The detection rate of the vanM resistance gene in Enterococcus faecium has increased year by year, and its horizontal transfer may have contributed to the increased prevalence of Enterococcus faecium co-harboring both vanA and vanM resistance genes in recent years.

, authors=Bo SU, Bo ZHENG, authorsList=Bo SU, Bo ZHENG, authorCompany=null, correspAuthors=Bo ZHENG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291707024371376397, articleId=1291707023847088395, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=同时携带vanAvanM耐药基因屎肠球菌流行现状研究, columnId=1246531407485489349, journalTitle=中国临床药理学杂志, columnName=临床与基础桥接研究, runingTitle=null, highlight=null, articleAbstract=
目的

对2011-2024年收集的2 307株屎肠球菌进行耐药表型和基因型分析。

方法

用微量肉汤稀释法对菌株进行药敏试验,后提取菌株基因组,进行聚合酶链反应(PCR)扩增筛查耐药基因。

结果

2011-2012年万古霉素耐药屎肠球菌检出率为1.67%,vanM基因阳性万古霉素敏感菌株检出率为0.34%,而2023-2024年万古霉素耐药屎肠球菌检出率增长为5.11%,vanM基因阳性万古霉素敏感菌株检出率高达4.75%。2021-2024年检测出22株同时携带vanAvanM耐药基因的屎肠球菌。

结论

屎肠球菌中vanM型耐药基因检出率逐年上升,其水平转移可能加剧近年同时携带vanAvanM耐药基因屎肠球菌的流行。

, authors=苏博, 郑波, authorsList=苏博, 郑波, authorCompany=null, correspAuthors=郑波, authorNote=

苏博(1999-),男,硕士研究生,主要从事细菌耐药机制研究

, correspAuthorsNote=
郑波,主任医师,博士生导师 Tel: (010)82802542 E-mail:
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苏博(1999-),男,硕士研究生,主要从事细菌耐药机制研究

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苏博(1999-),男,硕士研究生,主要从事细菌耐药机制研究

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同时携带vanAvanM耐药基因屎肠球菌流行现状研究
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苏博 , 郑波
中国临床药理学杂志 | 临床与基础桥接研究 2025,41(21): 3038-3042
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中国临床药理学杂志 |临床与基础桥接研究 2025 , 41 (21) : 3038 -3042
同时携带vanAvanM耐药基因屎肠球菌流行现状研究
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苏博(1999-),男,硕士研究生,主要从事细菌耐药机制研究

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苏博, 郑波
作者信息
  • 北京大学 第一医院 临床药理研究所,北京 100034
通讯作者:
郑波,主任医师,博士生导师 Tel: (010)82802542 E-mail:
作者简介:

苏博(1999-),男,硕士研究生,主要从事细菌耐药机制研究

Epidemiological landscape of Enterococcus faecium co-harboring both vanA and vanM resistance genes
Bo SU, Bo ZHENG
Affiliations
  • Institute of Clinical Pharmacology, Peking University First Hospital, Beijing 100034, China
出版时间: 2025-11-17 doi: 10.13699/j.cnki.1001-6821.2025.21.007
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目的

对2011-2024年收集的2 307株屎肠球菌进行耐药表型和基因型分析。

方法

用微量肉汤稀释法对菌株进行药敏试验,后提取菌株基因组,进行聚合酶链反应(PCR)扩增筛查耐药基因。

结果

2011-2012年万古霉素耐药屎肠球菌检出率为1.67%,vanM基因阳性万古霉素敏感菌株检出率为0.34%,而2023-2024年万古霉素耐药屎肠球菌检出率增长为5.11%,vanM基因阳性万古霉素敏感菌株检出率高达4.75%。2021-2024年检测出22株同时携带vanAvanM耐药基因的屎肠球菌。

结论

屎肠球菌中vanM型耐药基因检出率逐年上升,其水平转移可能加剧近年同时携带vanAvanM耐药基因屎肠球菌的流行。

万古霉素  /  屎肠球菌  /  vanA基因  /  vanM基因
Objective

To analyze the drug resistance phenotypes and genotypes of 2 307 Enterococcus faecium strains collected from 2011 to 2024.

Methods

The antimicrobial susceptibility testing of the strains was performed using the broth microdilution method, followed by genomic DNA extraction from the strains and polymerase chain reaction (PCR) amplification for screening of resistance genes.

Results

From 2011 to 2012, the detection rate of vancomycin-resistant Enterococcus faecium was 1.67%, and the detection rate of vanM gene-positive vancomycin-susceptible strains was 0.34%. In contrast, from 2023 to 2024, the detection rate of vancomycin-resistant Enterococcus faecium increased to 5.11%, while the detection rate of vanM gene-positive vancomycin-susceptible strains reached as high as 4.75%. Additionally, 22 Enterococcus faecium strains co-harboring both vanA and vanM resistance genes were identified during the period from 2021 to 2024.

Conclusion

The detection rate of the vanM resistance gene in Enterococcus faecium has increased year by year, and its horizontal transfer may have contributed to the increased prevalence of Enterococcus faecium co-harboring both vanA and vanM resistance genes in recent years.

vancomycin  /  Enterococcus faecium  /  vanA gene  /  vanM gene
苏博, 郑波. 同时携带vanAvanM耐药基因屎肠球菌流行现状研究. 中国临床药理学杂志, 2025 , 41 (21) : 3038 -3042 . DOI: 10.13699/j.cnki.1001-6821.2025.21.007
Bo SU, Bo ZHENG. Epidemiological landscape of Enterococcus faecium co-harboring both vanA and vanM resistance genes[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (21) : 3038 -3042 . DOI: 10.13699/j.cnki.1001-6821.2025.21.007

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doi: 10.13699/j.cnki.1001-6821.2025.21.007
  • 接收时间:2025-08-08
  • 首发时间:2026-08-05
  • 出版时间:2025-11-17
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  • 收稿日期:2025-08-08
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    北京大学 第一医院 临床药理研究所,北京 100034

通讯作者:

郑波,主任医师,博士生导师 Tel: (010)82802542 E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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