Article(id=1291704776329285786, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291704771212234905, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.17.009, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1737734400000, receivedDateStr=2025-01-25, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785896017380, onlineDateStr=2026-08-05, pubDate=1758038400000, pubDateStr=2025-09-17, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785896017380, onlineIssueDateStr=2026-08-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785896017380, creator=13701087609, updateTime=1785896017380, updator=13701087609, issue=Issue{id=1291704771212234905, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='17', pageStart='2401', pageEnd='2550', issueExtLink='null', onlineDate='null', pubDate='1758038400000', pubDateStr='2025-09-17', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785896016147, creator='13701087609', updateTime=1785896900366, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1291708479866164175, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291704771212234905, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1291708479866164176, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291704771212234905, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=2446, endPage=2452, ext={EN=ArticleExt(id=1291704777428193435, articleId=1291704776329285786, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Research on hydrogen sulfide promoting osteogenic differentiation of MC3TC-E1 cells by regulating macrophage polarization, columnId=1246531407326105792, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Clinical and Basic Bridging Research, runingTitle=null, highlight=null, articleAbstract=
Objective To investigate the effect and of hydrogen sulfide (H2S) on the osteogenic differentiation of osteoblast precursor cells (MC3T3-E1) by regulating the polarization of macrophages (RAW264.7).
Methods RAW264.7 cells were divided into four groups (n=10 in each group), including control group (untreated), M1 induction group [added with 100 ng·mL-1 lipopolysaccharide (LPS)], M2 induction group (added with 20 ng·mL-1 interleukin-4+20 ng·mL-1 interleukin-10, IL-4+IL-10), GYY4137 intervention group (H2S donor GYY4137 100 μmol·L-1). Reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blot were used to detect the mRNA and protein expression levels of M1 macrophage related factors including tumor necrosis factor-α (TNF-α), interleukin-1 β (IL-1β) and inducible nitric oxide synthase (i-NOS) and M2 macrophage related factors including interleukin-10 (IL-10), arginase-1 (Arg-1) and mannose receptor (CD206), and to verify the effect of polarization induction and the intervention of GYY4137; cell counting kit-8 (CCK-8) was used to detect the activity of RAW264.7 cells treated with GYY4137 at different concentrations for 24 h and 72 h (6 wells in each group). The expression levels of Arg-1 and CD206 in control group and GYY4137 group (100 μmol·L-1) were detected by flow cytometry (n=10 in each group). MC3T3-E1 cells were divided into four groups (n=10), which were blank group (without osteogenic induction medium), osteogenic control group (with osteogenic induction medium), co-culture group (co-cultured with RAW264.7 cells Transwell) and GYY4137 pretreatment co-culture group (co-cultured with 100 μmol·L-1 GYY4137 pretreated RAW264.7 cells). The mRNA expression levels of osteogenic related genes including Runt-related transcription factor 2 (Runx-2), osteopontin (OPN), bone morphogenetic protein-2 (BMP-2) and bone sialoprotein (BSP) mRNA were detected by RT-qPCR; alkaline phosphatase (ALP) staining and alizarin red staining were used to detect osteogenic differentiation.
Results The relative expression levels of TNF-α mRNA in control group, M1 induction group and GYY4137 intervention group were 1.56±0.22, 16.49±3.56 and 0.57±0.11, respectively; the relative expression levels of IL-1β mRNA were 1.06±0.24, 3.37±0.76 and 1.43±0.34, respectively. The above indexes in M1 induced group were significantly higher than those in control group, and the above indexes in GYY4137 intervention group were significantly lower than those in M1 induced group (all P<0.001). The relative expression levels of Arg-1 mRNA in GYY4137 group and control group were 1.38±0.36 and 0.91±0.12, respectively; the relative expression levels of CD206 mRNA were 17.43±2.84 and 1.14±0.21, respectively; the relative expression levels of CD206 protein were 0.89±0.25 and 0.20±0.04, respectively; the relative expression levels of i-NOS protein were 0.72±0.19 and 0.25±0.07, respectively; the relative expression levels of Arg-1 were 28.65±3.21 and 12.31±2.15, respectively; the relative expression levels of CD206 were 31.24±2.89 and 10.8±1.98, respectively. The above indexes in GYY4137 intervention group were significantly higher than those in control group, and the differences were statistically significant (P<0.05, P<0.001). The relative expression levels of Runx-2 mRNA in GYY4137 pretreatment and co-culture groups were 3.21±0.45 and 1.56±0.22, respectively; the relative expression levels of OPN mRNA were 2.89±0.38 and 1.43±0.19, respectively; the relative expression levels of BMP-2 mRNA were 3.56±0.52 and 1.67±0.25, respectively; the relative expression levels of BSP mRNA were 2.98±0.41 and 1.51±0.20, respectively. The above indexes in GYY4137 pretreatment co-culture group were significantly higher than those in co-culture group, and the differences were statistically significant (all P<0.05). The OD values of ALP in GYY4137 pretreatment co-culture group and osteogenic control group were (0.89±0.12) and (0.56±0.08) μm2, respectively; the areas of alizarin red stained calcium nodules were (2 865.32±215.67) and (1 568.45±189.32) μm2, respectively; the above indexes of GYY4137 pretreatment co-culture group were significantly higher than those of osteogenic control group, and the differences were statistically significant (all P<0.05).
Conclusion 100 μmol·L-1 GYY4137 can enhance the osteogenic differentiation of MC3T3-E1 cells by promoting the polarization of RAW264.7 cells to M2 macrophages, up regulating the expression of osteogenic related genes.
, authors=Meng-zhen LI
1, Da-wei HE
2a, Yan-ming HAO
2b, Chong LI
2c, Yi YIN
2c, authorsList=Meng-zhen LI, Da-wei HE, Yan-ming HAO, Chong LI, Yi YIN, authorCompany=null, correspAuthors=Yi YIN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291704780758470812, articleId=1291704776329285786, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=硫化氢通过调节巨噬细胞极化促进MC3TC-E1细胞成骨分化的研究, columnId=1246531407485489349, journalTitle=中国临床药理学杂志, columnName=临床与基础桥接研究, runingTitle=null, highlight=null, articleAbstract=
目的 探讨硫化氢（H2S）通过调控巨噬细胞（RAW264.7细胞株）极化对成骨前体细胞（MC3T3-E1细胞株）成骨分化的影响及其机制。
方法 将RAW264.7细胞接种后分为4组（每组n=10），分别为对照组（未处理）、M1诱导组[加入100 ng·mL-1脂多糖（LPS）]、M2诱导组（加入20 ng·mL-1白细胞介素-4+20 ng·mL-1白细胞介素-10，IL-4+IL-10）、GYY4137干预组（H2S供体GYY4137 100 μmol·L-1）。用逆转录定量聚合酶链反应（RT-qPCR）和蛋白质印迹法（Western blot）检测包括肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和诱导型一氧化氮合酶（i-NOS）在内的M1型巨噬细胞相关因子的mRNA及蛋白表达和包括白细胞介素-10（IL-10）、精氨酸酶-1（Arg-1）和甘露糖受体（CD206）在内的M2型巨噬细胞相关因子的mRNA及蛋白表达，并验证极化诱导效果及GYY4137的干预作用；用细胞计数试剂盒-8（CCK-8）实验法检测不同浓度GYY4137处理RAW264.7细胞24和72 h后的活性（每组6个复孔）。用流式细胞术检测对照组和GYY4137组（100 μmol·L-1）M2型标志物Arg-1和CD206表达（每组n=10）。将MC3T3-E1细胞分为4组（n=10），分别为空白组（无成骨诱导培养基）、成骨对照组（加成骨诱导培养基）、共培养组（与RAW264.7细胞Transwell共培养）和GYY4137预处理共培养组（与100 μmol·L-1GYY4137预处理的RAW264.7细胞共培养）。用RT-qPCR法检测包括Runt相关转录因子2（Runx-2）、骨桥蛋白（OPN）、骨形态发生蛋白-2（BMP-2）和骨唾液酸蛋白（BSP）在内的成骨相关基因mRNA相对表达水平；用碱性磷酸酶（ALP）染色法和茜素红染色法检测成骨分化能力。
结果 对照组、M1诱导组和GYY4137干预组的TNF-α mRNA相对表达水平分别为1.56±0.22、16.49±3.56和0.57±0.11；IL-1β mRNA相对表达水平分别为1.06±0.24、3.37±0.76和1.43±0.34，M1诱导组的上述指标均显著高于对照组，GYY4137干预组的上述指标均显著低于M1诱导组（均P<0.001）。GYY4137组和对照组的Arg-1 mRNA相对表达水平分别为1.38±0.36和0.91±0.12；CD206 mRNA相对表达水平分别为17.43±2.84和1.14±0.21；CD206蛋白相对表达水平分别为0.89±0.25和0.20±0.04；i-NOS蛋白相对表达水平分别为0.72±0.19和0.25±0.07；Arg-1相对表达水平分别为28.65±3.21和12.31±2.15；CD206相对表达水平分别为31.24±2.89和10.87±1.98，GYY4137干预组的上述指标均显著高于对照组，在统计学上差异均有统计学意义（P<0.05，P<0.001）。GYY4137预处理共培养组和共培养组的Runx-2 mRNA相对表达水平分别为3.21±0.45和1.56±0.22；OPN mRNA相对表达水平分别为2.89±0.38和1.43±0.19；BMP-2 mRNA相对表达水平分别为3.56±0.52和1.67±0.25；BSP mRNA相对表达水平分别为2.98±0.41和1.51±0.20，GYY4137预处理共培养组的上述指标均显著高于共培养组，在统计学上差异均有统计学意义（均P<0.05）。GYY4137预处理共培养组和成骨对照组的ALP的光密度（OD）值分别为（0.89±0.12）和（0.56±0.08）μm2；茜素红染色钙结节面积分别为（2 865.32±215.67）和（1 568.45±189.32）μm2；GYY4137预处理共培养组的上述指标均显著高于成骨对照组，在统计学上差异均有统计学意义（均P<0.05）。
结论 100 μmol·L-1 GYY4137可通过促进RAW264.7细胞向M2型巨噬细胞极化，上调成骨相关基因表达，增强MC3T3-E1细胞成骨分化能力。
, authors=李梦贞
1, 何大伟
2a, 郝彦明
2b, 李翀
2c, 尹毅
2c, authorsList=李梦贞, 何大伟, 郝彦明, 李翀, 尹毅, authorCompany=null, correspAuthors=尹毅, authorNote=
李梦贞（2000-），女，硕士研究生，主要从事脊柱外科和骨质疏松方向的相关研究
, correspAuthorsNote=
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=1gQzA1NnqI3awldIFHp3Zg==, magXml=oJ2NuQtd/SoJxXoOxGz77w==, pdfUrl=null, pdf=YKYw/3kE2Tgl3mZhPUvZ2g==, pdfFileSize=1421294, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=N9p2nCMAo5csLPUQ+b7zuA==, mapNumber=null, fund=null)}, authors=[Author(id=1291704781807046826, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291704781890932908, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704781807046826, language=EN, stringName=Meng-zhen LI, firstName=Meng-zhen, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
1, address=
1.School of Medicine, Jiangsu University, Zhenjiang 212000, Jiangsu Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291704781970624685, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704781807046826, language=CN, stringName=李梦贞, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
1, address=
1.江苏大学 医学院，江苏 镇江 212000, bio={"content":"
李梦贞（2000-），女，硕士研究生，主要从事脊柱外科和骨质疏松方向的相关研究
"}, bioImg=null, bioContent=
李梦贞（2000-），女，硕士研究生，主要从事脊柱外科和骨质疏松方向的相关研究
, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291704781345673373, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=1., ext=[AuthorCompanyExt(id=1291704781370839198, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781345673373, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
1.School of Medicine, Jiangsu University, Zhenjiang 212000, Jiangsu Province, China), AuthorCompanyExt(id=1291704781396005023, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781345673373, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
1.江苏大学 医学院，江苏 镇江 212000)])]), Author(id=1291704782088065199, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291704782163562673, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704782088065199, language=EN, stringName=Da-wei HE, firstName=Da-wei, middleName=null, lastName=HE, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2a, address=
2a.Clinical Experimental Research Center, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291704782222282930, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704782088065199, language=CN, stringName=何大伟, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2a, address=
2a.江苏大学 附属昆山医院，临床实验研究中心，江苏 昆山 215300, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291704781517639840, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=2a., ext=[AuthorCompanyExt(id=1291704781546999969, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781517639840, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2a.Clinical Experimental Research Center, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China), AuthorCompanyExt(id=1291704781559582882, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781517639840, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2a.江苏大学 附属昆山医院，临床实验研究中心，江苏 昆山 215300)])]), Author(id=1291704783900004532, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291704783983890614, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704783900004532, language=EN, stringName=Yan-ming HAO, firstName=Yan-ming, middleName=null, lastName=HAO, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2b, address=
2b.Department of Joint Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291704784046805175, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704783900004532, language=CN, stringName=郝彦明, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2b, address=
2b.江苏大学 附属昆山医院，关节外科，江苏 昆山 215300, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291704781630886051, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=2b., ext=[AuthorCompanyExt(id=1291704781639274660, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781630886051, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2b.Department of Joint Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China), AuthorCompanyExt(id=1291704781651857573, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781630886051, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2b.江苏大学 附属昆山医院，关节外科，江苏 昆山 215300)])]), Author(id=1291704784126496953, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291704784243937467, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704784126496953, language=EN, stringName=Chong LI, firstName=Chong, middleName=null, lastName=LI, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2c, address=
2c.Department of Spinal Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291704784327823548, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704784126496953, language=CN, stringName=李翀, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2c, address=
2c.江苏大学 附属昆山医院，脊柱外科，江苏 昆山 215300, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291704781714772134, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=2c., ext=[AuthorCompanyExt(id=1291704781723160743, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781714772134, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2c.Department of Spinal Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China), AuthorCompanyExt(id=1291704781731549352, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781714772134, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2c.江苏大学 附属昆山医院，脊柱外科，江苏 昆山 215300)])]), Author(id=1291704784541733054, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=yy-19723@163.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1291704784617230528, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704784541733054, language=EN, stringName=Yi YIN, firstName=Yi, middleName=null, lastName=YIN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2c, address=
2c.Department of Spinal Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291704784684339393, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, authorId=1291704784541733054, language=CN, stringName=尹毅, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
2c, address=
2c.江苏大学 附属昆山医院，脊柱外科，江苏 昆山 215300, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291704781714772134, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=2c., ext=[AuthorCompanyExt(id=1291704781723160743, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781714772134, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2c.Department of Spinal Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China), AuthorCompanyExt(id=1291704781731549352, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781714772134, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2c.江苏大学 附属昆山医院，脊柱外科，江苏 昆山 215300)])])], keywords=[Keyword(id=1291704784797585602, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=EN, orderNo=1, keyword=hydrogen sulfide), Keyword(id=1291704784864694467, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=EN, orderNo=2, keyword=macrophage), Keyword(id=1291704784956969156, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=EN, orderNo=3, keyword=polarization), Keyword(id=1291704785040855237, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=EN, orderNo=4, keyword=osteogenesis), Keyword(id=1291704785112158406, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=EN, orderNo=5, keyword=osteogenic precursor cell), Keyword(id=1291704785376399559, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=CN, orderNo=1, keyword=硫化氢), Keyword(id=1291704785477062856, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=CN, orderNo=2, keyword=巨噬细胞), Keyword(id=1291704785535783113, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=CN, orderNo=3, keyword=极化), Keyword(id=1291704785795829962, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=CN, orderNo=4, keyword=成骨), Keyword(id=1291704785883910347, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, language=CN, orderNo=5, keyword=成骨前体细胞)], refs=null, funds=[Fund(id=1291704786030710988, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, awardId=2020YFC2006100, language=CN, fundingSource=国家重点研发计划基金资助项目(2020YFC2006100), fundOrder=null, country=null), Fund(id=1291704786102014157, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, awardId=S23-075-101127, language=CN, fundingSource=江苏省社会发展基金资助项目(S23-075-101127), fundOrder=null, country=null), Fund(id=1291704786282369230, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, awardId=KZ2023005, language=CN, fundingSource=昆山市骨质疏松性骨折防治重点实验室基金资助项目(KZ2023005), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1291704781345673373, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=1., ext=[AuthorCompanyExt(id=1291704781370839198, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781345673373, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
1.School of Medicine, Jiangsu University, Zhenjiang 212000, Jiangsu Province, China), AuthorCompanyExt(id=1291704781396005023, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781345673373, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
1.江苏大学 医学院，江苏 镇江 212000)]), AuthorCompany(id=1291704781517639840, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=2a., ext=[AuthorCompanyExt(id=1291704781546999969, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781517639840, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2a.Clinical Experimental Research Center, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China), AuthorCompanyExt(id=1291704781559582882, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781517639840, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2a.江苏大学 附属昆山医院，临床实验研究中心，江苏 昆山 215300)]), AuthorCompany(id=1291704781630886051, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=2b., ext=[AuthorCompanyExt(id=1291704781639274660, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781630886051, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2b.Department of Joint Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China), AuthorCompanyExt(id=1291704781651857573, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781630886051, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2b.江苏大学 附属昆山医院，关节外科，江苏 昆山 215300)]), AuthorCompany(id=1291704781714772134, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, xref=2c., ext=[AuthorCompanyExt(id=1291704781723160743, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781714772134, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2c.Department of Spinal Surgery, Kunshan Hospital Affiliated to Jiangsu University, Kunshan 215300, Jiangsu Province, China), AuthorCompanyExt(id=1291704781731549352, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291704776329285786, companyId=1291704781714772134, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=
2c.江苏大学 附属昆山医院，脊柱外科，江苏 昆山 215300)])], figs=null, attaches=null, journal=Journal(id=1246415058658443328, delFlag=0, nameCn=中国临床药理学杂志, nameEn=Chinese Journal of Clinical Pharmacology, nameHistory1=null, nameHistory2=null, issn=1001-6821, eissn=null, cn=11-2220/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=8cEfS7jvj/ukyhKVOMJePA==, journalPrice=null, startedYear=null, abbrevIsoEn=Chinese Journal of Clinical Pharmacology, journalRemark=null, publicationField=null, createdTime=1775098107259, updatedTime=1788948995538, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Z, firstLetterEn=Z, subjectCode=Medical and Pharmaceutical Sciences, subjectName=null, subjectCodeEn=Medical and Pharmaceutical Sciences, subjectNameEn=null, picCn=8cEfS7jvj/ukyhKVOMJePA==, picEn=cQ12tu98gbNetbFuefeYGg==, jcr=null, cjcr=null, exts=[JournalExt(id=1304509895005466898, language=CN, name=中国临床药理学杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788948995591, updatedTime=1788948995591, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://lcylauthor.cast.org.cn/webm, submissionEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionReviewUrl=https://lcylauthor.cast.org.cn/webm, submissionCeEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionAeEditorUrl=https://lcyleditor.cast.org.cn/webm, option={"copyright":""}), JournalExt(id=1304509895047409939, language=EN, name=Chinese Journal of Clinical Pharmacology, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788948995601, updatedTime=1788948995601, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://lcylauthor.cast.org.cn/webm, submissionEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionReviewUrl=https://lcylauthor.cast.org.cn/webm, submissionCeEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionAeEditorUrl=https://lcyleditor.cast.org.cn/webm, option={"copyright":""})], databaseList=null, tenantJournalId=1246415772164075586, websiteList=[Website(id=1246421196657546140, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1246415772164075586, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN, language=CN, createTime=1775099570670, createBy=18614031015, updateTime=1775099747371, updateBy=18614031015, name=中国临床药理学杂志-中文, tplId=1146099689490845704, title=中国临床药理学杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1246422027293316008, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=articleTextType, value=kx, createTime=1775099768708, updateTime=1775099768708, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027268150181, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=banner, value=null, createTime=1775099768702, updateTime=1775099768702, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027310093227, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=grayFlag, value=0, createTime=1775099768712, updateTime=1775099768712, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027259761572, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=logo, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/file/pic?fileId=KeUk4pyVjfIIkOq40uZX0Q==, createTime=1775099768700, updateTime=1775099768700, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027322676141, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=minRunFlag, value=0, createTime=1775099768715, updateTime=1775099768715, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027284927399, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/file/pic, createTime=1775099768706, updateTime=1775099768706, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027318481836, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=silenceFlag, value=0, createTime=1775099768714, updateTime=1775099768714, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027276538790, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1775099768704, updateTime=1775099768704, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027297510313, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=themeColor, value=null, createTime=1775099768709, updateTime=1775099768709, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027305898922, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=themeStyle, value=null, createTime=1775099768711, updateTime=1775099768711, creator=18614031015, updator=18614031015)]), Website(id=1246421196871455649, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1246415772164075586, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN, language=EN, createTime=1775099570720, createBy=18614031015, updateTime=1775099757298, updateBy=18614031015, name=中国临床药理学杂志-英文, tplId=1146101810881728533, title=Chinese Journal of Clinical Pharmacology, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1246422058998059954, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=articleTextType, value=kx, createTime=1775099776267, updateTime=1775099776267, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058977088431, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=banner, value=null, createTime=1775099776262, updateTime=1775099776262, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059014837173, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=grayFlag, value=0, createTime=1775099776271, updateTime=1775099776271, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058968699822, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=logo, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/file/pic?fileId=KeUk4pyVjfIIkOq40uZX0Q==, createTime=1775099776260, updateTime=1775099776260, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059031614391, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=minRunFlag, value=0, createTime=1775099776275, updateTime=1775099776275, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058993865649, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/file/pic, createTime=1775099776266, updateTime=1775099776266, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059023225782, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=silenceFlag, value=0, createTime=1775099776273, updateTime=1775099776273, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058985477040, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1775099776264, updateTime=1775099776264, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059002254259, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=themeColor, value=null, createTime=1775099776268, updateTime=1775099776268, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059010642868, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=themeStyle, value=null, createTime=1775099776270, updateTime=1775099776270, creator=18614031015, updator=18614031015)])], journalTitle=中国临床药理学杂志, weixinUrl=null, journalUrl=null, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Journal of Clinical Pharmacology, journalPhotoCn=8cEfS7jvj/ukyhKVOMJePA==, journalPhotoEn=cQ12tu98gbNetbFuefeYGg==, journalFirstLetter=Z, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.17.009, detailUrlEn=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/10.13699/j.cnki.1001-6821.2025.17.009, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/PDF/10.13699/j.cnki.1001-6821.2025.17.009, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/PDF/10.13699/j.cnki.1001-6821.2025.17.009, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1758038400000, fullTextJson=null, articleText=null, reference=null)
PDF下载
全屏
113
23
关闭全屏
北京市海淀区学院南路86号
010-62199257（仅加盟咨询）
qkjq@cast.org.cn
