中国临床药理学杂志

水菖蒲通过抑制MAPK/NF-κB信号通路对大鼠放射性皮肤损伤创面愈合的研究

PDF下载

武晓丹 ^1a^,^1b^,^1c^,^1d , 童亚楠 ^1a^,^1b^,^1c^,^1d , 战莹 ^1a^,^1b^,^1c^,^1d , 张文文 ^1a^,^1b^,^1c^,^1d , 王治国 ^1a^,^1b^,^1c^,^1d , 张国旭 ^1a^,^1b^,^1c^,^1d

中国临床药理学杂志 | 临床与基础桥接研究 2025,41(8): 1138-1143

收起

中国临床药理学杂志 |临床与基础桥接研究 2025 , 41 (8) : 1138 -1143

水菖蒲通过抑制MAPK/NF-κB信号通路对大鼠放射性皮肤损伤创面愈合的研究

全屏

武晓丹^1a^,^1b^,^1c^,^1d, 童亚楠^1a^,^1b^,^1c^,^1d, 战莹^1a^,^1b^,^1c^,^1d, 张文文^1a^,^1b^,^1c^,^1d, 王治国^1a^,^1b^,^1c^,^1d, 张国旭^1a^,^1b^,^1c^,^1d

作者信息

^1a.中国人民解放军北部战区总医院，核医学科，辽宁沈阳 110016

^1b.中国人民解放军北部战区总医院，核背景复合战创伤救治实验室，辽宁沈阳 110016

^1c.中国人民解放军北部战区总医院，辽宁省核医学分子影像重点实验室，辽宁沈阳 110016

^1d.中国人民解放军北部战区总医院，联勤医学优质学科，辽宁沈阳 110016

通讯作者:

张国旭，主任医师，博士生导师 Tel: （024）28856372 E-mail: zhangguoxu502@sina.com

作者简介:

武晓丹（1991-），女，主管药师，主要从事放射性皮肤损伤辐射防护的相关研究

Research of Acorus calamus L. inhibiting MAPK/NF-κB signaling pathway on healing of radioactive skin injury in rats

Xiao-dan WU^1a^,^1b^,^1c^,^1d, Ya-nan TONG^1a^,^1b^,^1c^,^1d, Ying ZHAN^1a^,^1b^,^1c^,^1d, Wen-wen ZHANG^1a^,^1b^,^1c^,^1d, Zhi-guo WANG^1a^,^1b^,^1c^,^1d, Guo-xu ZHANG^1a^,^1b^,^1c^,^1d

Affiliations

^1a.Department of Nuclear Medicine, General Hospital of Northern Theater Command, Shenyang 110016, Liaoning Province, China

^1b.Nuclear Background Compound War Trauma Treatment Laboratory, General Hospital of Northern Theater Command, Shenyang 110016, Liaoning Province, China

^1c.Liaoning Key Laboratory of Nuclear Medicine Molecular Imaging, General Hospital of Northern Theater Command, Shenyang 110016, Liaoning Province, China

^1d.High Quality Disciplines of Department of General Logistics Medicine, General Hospital of Northern Theater Command, Shenyang 110016, Liaoning Province, China

出版时间: 2025-04-28 doi: 10.13699/j.cnki.1001-6821.2025.08.016

文章导航

摘要

收起

目的

基于丝裂原活化蛋白激酶（MAPK）/核因子κB（NF-κB）信号通路探讨水菖蒲对大鼠放射性皮肤损伤创面愈合的作用。

方法

用放射性核素照射法构建放射性皮肤损伤大鼠模型，将造模成功的大鼠随机分为模型组、对照组和高、中、低剂量实验组，每组12只；另取12只正常大鼠作为正常组。高、中、低剂量实验组分别外敷600、400、200 g·kg^-1水菖蒲；对照组外敷400 g·kg^-1三乙醇胺乳膏；正常组和模型组均外敷0.9% NaCl。6组大鼠每天给药1次，连续给药45 d。比较各组大鼠创面愈合率，用酶联免疫吸附试验法检测血清中白细胞介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α以及创面组织中羟脯氨酸（HYP）含量，用蛋白质印迹法测定创面组织中IL-1β、IL-6、TNF-α、丝裂原活化蛋白激酶（p38）和核因子κB p65亚单位（p65）蛋白的表达水平。

结果

高、中、低剂量实验组和对照组、模型组的创面愈合率分别为（70.24±3.17）%、（62.41±4.52）%、（49.89±4.61）%、（50.35±2.44）%和（29.42±4.23）%；高、中、低剂量实验组和对照组、模型组和正常组血清中IL-1β水平分别为（41.22±2.98）、（52.35±1.69）、（61.32±2.74）、（45.26±3.12）、（79.24±5.64）和（28.85±2.11）pg·L^-1，IL-6水平分别为（25.68±1.65）、（32.55±2.64）、（51.23±2.34）、（32.32±1.56）、（61.12±2.35）和（18.88±1.12）pg·L^-1，TNF-α水平分别为（62.38±5.66）、（73.56±4.52）、（88.63±5.68）、（68.33±4.32）、（112.48±6.52）和（55.21±3.89）pg·L^-1，创面组织中HYP水平分别为（3.77±0.41）、（3.51±0.26）、（2.98±0.39）、（3.62±0.37）、（2.21±0.48）和（4.22±0.55）μg·mg^-1，IL-1β蛋白相对表达水平分别为0.31±0.04、0.43±0.07、0.60±0.03、0.51±0.04、0.69±0.06和0.11±0.07，IL-6蛋白相对表达水平分别为0.50±0.03、0.60±0.04、0.69±0.04、0.62±0.07、0.83±0.08和0.28±0.06，TNF-α蛋白相对表达水平分别为0.49±0.06、0.61±0.03、0.73±0.06、0.59±0.04、0.83±0.05和0.28±0.04，磷酸化p38/p38比值分别为0.46±0.06、0.56±0.04、0.67±0.03、0.56±0.05、0.84±0.04和0.34±0.08，磷酸化p65/p65比值分别为0.37±0.06、0.54±0.03、0.63±0.04、0.47±0.05、0.79±0.04和0.32±0.06。高、中剂量实验组的上述指标与模型组比较，在统计学上差异均有统计学意义（均P<0.05）。

结论

水菖蒲能够促进大鼠放射性皮肤损伤创面愈合，抑制创面组织的炎症水平，促进胶原蛋白沉积，其机制可能与调节MAPK/NF-κB通路有关。

关键词

水菖蒲 / 放射性皮肤损伤 / 丝裂原活化蛋白激酶/核因子κB信号通路

Abstract

收起

Objective

To investigate the effect of Acorus calamus L. on wound healing of radioactive skin injury in rats based on the mitogen-activated protein kinase (MAPK)/nuclear factor κB (NF-κB) signaling pathway.

Methods

A rat model of radioactive skin injury was induced by the radionuclide irradiation, the successful model rats were randomly divided into the model group, the control group, and the experimental -H, -M, -L groups with 12 rats in each group, and another 12 normal rats were taken as the normal group. Experimental-H, -M, -L goups was applied with Acorus calamus L. 600, 400, 200 g·kg^-1, respectively. Control group was applied with triethanolamine cream 400 g·kg^-1 on rat wounds; normal and model groups were treated with 0.9% NaCl. Six groups were treated once a day for 45 days. The healing rate of wounds was compared among the six groups. The levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor (TNF)-α in serum and hydroxyproline (HYP) in wound tissue were measured by enzyme linked immunosorbent assay. The protein levels of IL-1β, IL-6, TNF-α, mitogen-activated protein kinase (p38) and nuclear factor κB p65 subunit (p65) were detected by Western blot.

Results

The wound healing rates of experimental-H, -M, -L groups, control group and model group were (70.24±3.17)%, (62.41±4.52)%, (49.89±4.61)%, (50.35±2.44)% and (29.42±4.23)%, respectively. The levels of IL-1β in experimental-H, -M, -L groups, control group, model group and normal group were (41.22±2.98), (52.35±1.69), (61.32±2.74), (45.26±3.12), (79.24±5.64) and (28.85±2.11) pg·L^-1; the levels of IL-6 were (25.68±1.65), (32.55±2.64), (51.23±2.34), (32.32±1.56), (61.12±2.35) and (18.88±1.12) pg·L^-1; the levels of TNF-α were (62.38±5.66), (73.56±4.52), (88.63±5.68), (68.33±4.32), (112.48±6.52) and (55.21±3.89) pg·L^-1; the levels of HYPs of (3.77±0.41), (3.51±0.26), (2.98±0.39), (3.62±0.37), (2.21±0.48) and (4.22±0.55) μg·mg^-1; the relative expression levels of IL-1β were 0.31±0.04, 0.43±0.07, 0.60±0.03, 0.51±0.04, 0.69±0.06 and 0.11±0.07; the relative expression levels of IL-6 were 0.50±0.03, 0.60±0.04, 0.69±0.04, 0.62±0.07, 0.83±0.08 and 0.28±0.06; the relative expression levels of TNF-α were 0.49±0.06, 0.61±0.03, 0.73±0.06, 0.59±0.04, 0.83±0.05 and 0.28±0.04; the phosphorylated p38/p38 ratios were 0.46±0.06, 0.56±0.04, 0.67±0.03, 0.56±0.05, 0.84±0.04 and 0.34±0.08; the phosphorylated p65/p65 ratios were 0.37±0.06, 0.54±0.03, 0.63±0.04, 0.47±0.05, 0.79±0.04, and 0.32±0.06, respectively. Compared with model group, the above indexes in the experimental-H, -M groups were statistically significant (all P<0.05).

Conclusion

Acorus calamus L. can promote the healing of radioactive skin injury in rats, inhibit the inflammation level of the wound tissue, and promote collagen deposition, and its mechanism may be related to the regulation of MAPK/NF-κB pathway.

Key words

Acorus calamus L. / radiation skin reaction / mitogen-activated protein kinase/nuclear factor kappa-B signaling pathway

引用本文

武晓丹, 童亚楠, 战莹, 张文文, 王治国, 张国旭. 水菖蒲通过抑制MAPK/NF-κB信号通路对大鼠放射性皮肤损伤创面愈合的研究. 中国临床药理学杂志, 2025 , 41 (8) : 1138 -1143 . DOI: 10.13699/j.cnki.1001-6821.2025.08.016

Xiao-dan WU, Ya-nan TONG, Ying ZHAN, Wen-wen ZHANG, Zhi-guo WANG, Guo-xu ZHANG. Research of Acorus calamus L. inhibiting MAPK/NF-κB signaling pathway on healing of radioactive skin injury in rats[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (8) : 1138 -1143 . DOI: 10.13699/j.cnki.1001-6821.2025.08.016

基金

收起

国家自然科学基金青年基金资助项目(82102744)
辽宁省民生科技计划联合计划基金资助项目(2022JH2/101500021)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

2025年第41卷第8期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.13699/j.cnki.1001-6821.2025.08.016

接收时间：2024-10-30
首发时间：2026-08-04
出版时间：2025-04-28

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2024-10-30

基金

国家自然科学基金青年基金资助项目(82102744)

辽宁省民生科技计划联合计划基金资助项目(2022JH2/101500021)

作者信息

^1a.中国人民解放军北部战区总医院，核医学科，辽宁沈阳 110016

^1b.中国人民解放军北部战区总医院，核背景复合战创伤救治实验室，辽宁沈阳 110016

^1c.中国人民解放军北部战区总医院，辽宁省核医学分子影像重点实验室，辽宁沈阳 110016

^1d.中国人民解放军北部战区总医院，联勤医学优质学科，辽宁沈阳 110016

通讯作者:

张国旭，主任医师，博士生导师 Tel: （024）28856372 E-mail: zhangguoxu502@sina.com

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.08.016

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT