Article(id=1291404575857173319, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291404554201985968, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.06.011, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1728489600000, receivedDateStr=2024-10-10, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785824444020, onlineDateStr=2026-08-04, pubDate=1743091200000, pubDateStr=2025-03-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785824444020, onlineIssueDateStr=2026-08-04, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785824444020, creator=13701087609, updateTime=1785824444020, updator=13701087609, issue=Issue{id=1291404554201985968, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='6', pageStart='751', pageEnd='900', issueExtLink='null', onlineDate='null', pubDate='1743091200000', pubDateStr='2025-03-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785824438858, creator='13701087609', updateTime=1785824438858, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=805, endPage=810, ext={EN=ArticleExt(id=1291404576821863240, articleId=1291404575857173319, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Research on GLPS alleviated H
2O
2-induced ferroptosis in mouse hippocampal neurons by regulating Nrf2/HO-1 signaling pathway, columnId=1246531407326105792, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Clinical and Basic Bridging Research, runingTitle=null, highlight=null, articleAbstract=
Objective To investigate the effect of ganoderma lucidum polysaccharides (GLPS) on ferroptosis of hippocampal neuronal (HT22) cell lines induced by hydrogen peroxide (H2O2) and its mechanism.
Methods The H2O2-induced HT22 cell injury model was first established in vitro. HT22 cells were divided into normal group (conventional culture), H2O2 group (200 μmol·L-1 H2O2), GLPS group (200 μmol·L-1 H2O2+100 μg·mL-1 GLPS), H2O2+Fin56 group (200 μmol·L-1 H2O2+5 μmol·L-1 Fin56), H2O2+Fin56+GLPS group (200 μmol·L-1 H2O2+5 μmol·L-1 Fin56+100 μg·mL-1 GLPS). The oxidative stress markers were detected by enzyme-linked immunosorbent assay (ELISA); the protein expression levels of iron death markers, nuclear factor erythroid2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were detected by Western blot.
Results The contents of malondialdehyde (MDA) in normal group, H2O2 group, GLPS group, H2O2+Fin56 group and H2O2+Fin56+GLPS group were (5.56±0.61), (15.53±0.83), (6.51±0.29), (20.92±1.03) and (13.65±0.70) nmol·mL-1; the contents of superoxide dismutase (SOD) were (37.93±1.37), (22.48±0.59), (37.81±2.56), (16.10±2.28) and (22.21±2.99) μg·mL-1; Ferritin protein expression levels were 1.52±0.03, 1.22±0.07, 1.42±0.06, 0.64±0.02 and 0.93±0.13; GPX4 protein expression levels were 3.29±0.34, 1.91±0.18, 2.99±0.22, 1.13±0.07 and 2.45±0.05; Nrf2 protein expression levels were 0.90±0.01, 2.24±0.02, 2.97±0.07, 2.45±0.07 and 2.76±0.11; HO-1 protein expression levels were 1.58±0.02, 1.76±0.04, 2.29±0.06, 1.67±0.02 and 2.03±0.06, respectively. H2O2 group was compared with normal group and H2O2+Fin56 group, GLPS group was compared with H2O2 group, H2O2+Fin56+GLPS group was compared with H2O2+Fin56 group and GLPS group, the differences were statistically significant (P<0.05, P<0.01).
Conclusion GLPS can improve lipid peroxide accumulation and iron metabolism disorder induced by H2O2 in HT22 cells by activating Nrf2/HO-1 pathway, thereby inhibiting the occurrence of ferroptosis.
, authors=Yan-qing LI
1, Zhi-chao YANG
1, Li-juan SONG
1, Qing WANG
1, Han-bin WANG
2, Li-zhi YANG
2, Bao-guo XIAO
3, Cun-gen MA
1, authorsList=Yan-qing LI, Zhi-chao YANG, Li-juan SONG, Qing WANG, Han-bin WANG, Li-zhi YANG, Bao-guo XIAO, Cun-gen MA, authorCompany=null, correspAuthors=Cun-gen MA, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291404577505534793, articleId=1291404575857173319, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=灵孢多糖通过调控Nrf2/HO-1信号途径减轻H
2O
2诱导小鼠海马神经元细胞铁死亡的研究, columnId=1246531407485489349, journalTitle=中国临床药理学杂志, columnName=临床与基础桥接研究, runingTitle=null, highlight=null, articleAbstract=
目的 探讨灵孢多糖(GLPS)对过氧化氢(H2O2)诱导的小鼠海马神经元(HT22)细胞铁死亡的影响及其机制。
方法 通过H2O2诱导建立细胞损伤模型。将HT22细胞分为正常组(常规培养)、H2O2组(200 μmol·L-1 H2O2)、GLPS组(200 μmol·L-1 H2O2+100 μg·mL-1 GLPS)、H2O2+Fin56组(200 μmol·L-1 H2O2+5 μmol·L-1 Fin56)、H2O2+Fin56+GLPS组(200 μmol·L-1 H2O2+5 μmol·L-1 Fin56+100 μg·mL-1 GLPS)。用酶联免疫吸附试验(ELISA)法检测氧化应激标志物水平,用蛋白质印迹法检测铁死亡标志物和核因子E2相关因子2(Nrf2)、血红素加氧酶-1(HO-1)的蛋白相对表达水平。
结果 正常组、H2O2组、GLPS组、H2O2+Fin56组和H2O2+Fin56+GLPS组中丙二醛(MDA)含量分别为(5.56±0.61)、(15.53±0.83)、(6.51±0.29)、(20.92±1.03)和(13.65±0.70)nmol·mL-1,超氧化物歧化酶(SOD)含量分别为(37.93±1.37)、(22.48±0.59)、(37.81±2.56)、(16.10±2.28)和(22.21±2.99)μg·mL-1,Ferritin蛋白相对表达水平分别为1.52±0.03、1.22±0.07、1.42±0.06、0.64±0.02和0.93±0.13,GPX4蛋白相对表达水平分别为3.29±0.34、1.91±0.18、2.99±0.22、1.13±0.07和2.45±0.05,Nrf2蛋白相对表达水平分别为0.90±0.01、2.24±0.02、2.97±0.07、2.45±0.07和2.76±0.11,HO-1蛋白相对表达水平分别为1.58±0.02、1.76±0.04、2.29±0.06、1.67±0.02和2.03±0.06。H2O2组的上述指标与正常组、H2O2+Fin56组比较,GLPS组的上述指标与H2O2组比较,H2O2+Fin56+GLPS组的上述指标与H2O2+Fin56组、GLPS组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。
结论 GLPS能够通过激活Nrf2/HO-1途径来改善H2O2诱导的HT22细胞脂质过氧化物积累和铁代谢紊乱,从而抑制铁死亡的发生。
, authors=李彦青
1, 杨智超
1, 宋丽娟
1, 王青
1, 王翰斌
2, 杨立志
2, 肖保国
3, 马存根
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李彦青(1988-),女,博士,讲师,主要从事神经药理学方面的研究
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1.山西中医药大学 神经生物学研究中心,国家中医药管理局多发性硬化益气活血重点研究室,山西 晋中 030619)])]), Author(id=1291404640931799231, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404575857173319, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291404641384784065, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404575857173319, authorId=1291404640931799231, language=EN, stringName=Zhi-chao YANG, firstName=Zhi-chao, middleName=null, lastName=YANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=
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