首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1291404566264800036, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291404554201985968, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.06.016, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1733241600000, receivedDateStr=2024-12-04, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785824441733, onlineDateStr=2026-08-04, pubDate=1743091200000, pubDateStr=2025-03-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785824441733, onlineIssueDateStr=2026-08-04, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785824441733, creator=13701087609, updateTime=1785824441733, updator=13701087609, issue=Issue{id=1291404554201985968, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='6', pageStart='751', pageEnd='900', issueExtLink='null', onlineDate='null', pubDate='1743091200000', pubDateStr='2025-03-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785824438858, creator='13701087609', updateTime=1785824438858, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=834, endPage=839, ext={EN=ArticleExt(id=1291404568366146342, articleId=1291404566264800036, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Protective effects of tacrolimus on chronic glomerulonephritis in rats, columnId=1246531407326105792, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Clinical and Basic Bridging Research, runingTitle=null, highlight=null, articleAbstract=
Objective

To explore the mechanism of tacrolimus mediating the protective effect on chronic glomerulonephritis (CGN) rats through regulating the expression of microRNA-145-5p (miR-145-5p) and modulating the C-X-C motif chemokine ligand 16/Ras homolog family member A/Rho-associated coiled-coil-containing protein kinase (CXCL16/RhoA/ROCK) pathway.

Methods

SD rats were randomly divided into the control group (administered an equal volume of 0.9% NaCl), model group (CGN model + equal volume of 0.9% NaCl), experimental group (CGN model + 3 mg·kg-1·d-1 tacrolimus) and combined group (experimental group + tail vein injection of 140 nmol·L-1 miR-145-5p antagomir). Enzyme-linked immunosorbent assay was used to measure 24-hour urinary protein (PRO), blood urea nitrogen (BUN), and serum creatinine (SCr) levels. Real-time quantitative polymerase chain reaction was used to detect the relative expression level of miR-145-5p in rat kidney tissue; Western blotting was used to determine protein expression levels.

Results

The 24-hour PRO levels in the control, model and experimental groups were (7.44±2.16), (85.29±17.35) and (49.81±9.52) mg, respectively; the BUN levels were (5.82±1.03), (9.65±1.78) and (6.97±1.14) mmol·L-1, respectively; the SCr levels were (46.31±7.69), (93.57±16.94) and (58.72±8.36) μmol·L-1, respectively. The relative expression levels of miR-145-5p in the control, model, experimental and combined groups were 1.00±0.14, 0.56±0.07, 0.83±0.12 and 0.68±0.09, respectively; the TNF-α levels were (116.42±14.53), (237.56±26.18), (164.79±18.35) and (205.12±21.64) pg·mL-1, respectively; the IL-1β levels were (15.76±1.84), (52.41±6.27), (21.35±2.92) and (43.63±4.58) pg·mL-1, respectively; the IL-6 levels were (26.28±3.47), (71.53±8.64), (38.92±5.06) and (59.78±6.21) pg·mL-1, respectively; the CXCL16 expression levels were 1.00±0.12, 1.62±0.17, 1.23±0.14 and 1.45±0.15, respectively. Statistical analysis revealed significant differences between the model group and the control group, between the experimental group and the model group, and between the combined group and the experimental group for these indicators (P<0.05, P<0.01, P<0.001).

Conclusion

Tacrolimus can upregulate miR-145-5p, activate the CXCL16/RhoA/ROCK pathway, reduce inflammatory factors, and improve kidney damage in CGN rats.

, authors=Wei WANG1, Meng-jie WANG1, Jian HUA2, authorsList=Wei WANG, Meng-jie WANG, Jian HUA, authorCompany=null, correspAuthors=Jian HUA, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291404569213395751, articleId=1291404566264800036, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=他克莫司对慢性肾小球肾炎大鼠的保护作用, columnId=1246531407485489349, journalTitle=中国临床药理学杂志, columnName=临床与基础桥接研究, runingTitle=null, highlight=null, articleAbstract=
目的

探究他克莫司通过调控微小RNA-145-5p（miR-145-5p）表达水平介导CXC趋化因子配体16/Ras同系物家族成员A/Rho相关激酶（CXCL16/RhoA/ROCK）通路对慢性肾小球肾炎（CGN）大鼠保护作用的机制。

方法

SD大鼠随机分为对照组（等量0.9% NaCl）、模型组（构建CGN模型+等量0.9% NaCl）、实验组（构建CGN模型+3 mg·kg-1·d-1他克莫司）和联合组（在实验组的基础上经尾静脉注射140 nmol·L-1 miR-145-5p antagomir）。用酶联免疫吸附试验法检测24 h尿蛋白（PRO）、血尿素氮（BUN）、血清肌酸酐（SCr）水平，用实时荧光定量聚合酶链反应法检测大鼠肾组织miR-145-5p的相对表达水平，用蛋白质印迹法检测蛋白相对表达水平。

结果

对照组、模型组、实验组的24 h PRO水平分别为（7.44±2.16）、（85.29±17.35）和（49.81±9.52）mg，BUN水平分别为（5.82±1.03）、（9.65±1.78）和（6.97±1.14）mmol·L-1，SCr水平分别为（46.31±7.69）、（93.57±16.94）和（58.72±8.36）μmol·L-1；对照组、模型组、实验组和联合组的miR-145-5p相对表达水平分别为1.00±0.14、0.56±0.07、0.83±0.12和0.68±0.09，TNF-α水平分别为（116.42±14.53）、（237.56±26.18）、（164.79±18.35）和（205.12±21.64）pg·mL-1，IL-1β水平分别为（15.76±1.84）、（52.41±6.27）、（21.35±2.92）和（43.63±4.58）pg·mL-1，IL-6水平分别为（26.28±3.47）、（71.53±8.64）、（38.92±5.06）和（59.78±6.21）pg·mL-1，CXCL16蛋白相对表达水平分别为1.00±0.12、1.62±0.17、1.23±0.14和1.45±0.15，模型组的上述指标与对照组相比，实验组的上述指标与模型组相比，联合组的上述指标和实验组相比，在统计学上差异均有统计学意义（P<0.05，P<0.01，P<0.001）。

结论

他克莫司可上调miR-145-5p激活CXCL16/RhoA/ROCK通路，减少炎性因子，改善CGN大鼠肾损伤。

, authors=王玮1, 王梦婕1, 华坚2, authorsList=王玮, 王梦婕, 华坚, authorCompany=null, correspAuthors=华坚, authorNote=

王玮（1980-），男，主管药师，主要从事医院药品方面工作及相关研究

, correspAuthorsNote=
华坚，副主任医师 MP: 13698671521 E-mail:
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=5mnen47+NErrP7wuwkZOlA==, magXml=vb5StB9ZNg1i6TLYLZ8mGA==, pdfUrl=null, pdf=JmOrjmoQXWnv4bJFd0TCbQ==, pdfFileSize=1707758, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=kXBau9kkMR4v8MWlCA5Nng==, mapNumber=null, fund=null)}, authors=[Author(id=1291404676033933808, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291404676281397746, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676033933808, language=EN, stringName=Wei WANG, firstName=Wei, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291404676352700915, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676033933808, language=CN, stringName=王玮, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033, bio={"content":"

王玮（1980-），男，主管药师，主要从事医院药品方面工作及相关研究

"}, bioImg=null, bioContent=

王玮（1980-），男，主管药师，主要从事医院药品方面工作及相关研究

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291404675887133161, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, xref=1., ext=[AuthorCompanyExt(id=1291404675895521770, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China), AuthorCompanyExt(id=1291404675908104683, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033)])]), Author(id=1291404676424004085, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291404676486918647, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676424004085, language=EN, stringName=Meng-jie WANG, firstName=Meng-jie, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291404676646302200, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676424004085, language=CN, stringName=王梦婕, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291404675887133161, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, xref=1., ext=[AuthorCompanyExt(id=1291404675895521770, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China), AuthorCompanyExt(id=1291404675908104683, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033)])]), Author(id=1291404676755354106, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=13698671521@163.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1291404677061538300, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676755354106, language=EN, stringName=Jian HUA, firstName=Jian, middleName=null, lastName=HUA, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.Department of Nephrology, Qingdao Municipal Hospital, Qingdao 266000, Shandong Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291404677128647165, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676755354106, language=CN, stringName=华坚, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.青岛市市立医院 肾内科，山东 青岛 266000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291404675962630636, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, xref=2., ext=[AuthorCompanyExt(id=1291404675971019245, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675962630636, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Department of Nephrology, Qingdao Municipal Hospital, Qingdao 266000, Shandong Province, China), AuthorCompanyExt(id=1291404675979407854, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675962630636, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛市市立医院 肾内科，山东 青岛 266000)])])], keywords=[Keyword(id=1291404677258670590, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=EN, orderNo=1, keyword=tacrolimus), Keyword(id=1291404677380305407, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=EN, orderNo=2, keyword=microRNA-145-5p), Keyword(id=1291404677480968704, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=EN, orderNo=3, keyword=CXC ligand 16), Keyword(id=1291404677581632001, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=EN, orderNo=4, keyword=Rho-associated protein kinase), Keyword(id=1291404679284519426, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=EN, orderNo=5, keyword=chronic glomerulonephritis), Keyword(id=1291404679355822595, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=CN, orderNo=1, keyword=他克莫司), Keyword(id=1291404679431320068, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=CN, orderNo=2, keyword=微小分子核糖核酸145-5p), Keyword(id=1291404679573926405, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=CN, orderNo=3, keyword=CXC配体16), Keyword(id=1291404679649423878, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=CN, orderNo=4, keyword=Rho相关蛋白激酶), Keyword(id=1291404679703949831, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, language=CN, orderNo=5, keyword=慢性肾小球肾炎)], refs=null, funds=null, companyList=[AuthorCompany(id=1291404675887133161, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, xref=1., ext=[AuthorCompanyExt(id=1291404675895521770, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China), AuthorCompanyExt(id=1291404675908104683, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033)]), AuthorCompany(id=1291404675962630636, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, xref=2., ext=[AuthorCompanyExt(id=1291404675971019245, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675962630636, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Department of Nephrology, Qingdao Municipal Hospital, Qingdao 266000, Shandong Province, China), AuthorCompanyExt(id=1291404675979407854, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675962630636, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛市市立医院 肾内科，山东 青岛 266000)])], figs=null, attaches=null, journal=Journal(id=1246415058658443328, delFlag=0, nameCn=中国临床药理学杂志, nameEn=Chinese Journal of Clinical Pharmacology, nameHistory1=null, nameHistory2=null, issn=1001-6821, eissn=null, cn=11-2220/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=8cEfS7jvj/ukyhKVOMJePA==, journalPrice=null, startedYear=null, abbrevIsoEn=Chinese Journal of Clinical Pharmacology, journalRemark=null, publicationField=null, createdTime=1775098107259, updatedTime=1784020014723, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Z, firstLetterEn=Z, subjectCode=Life Sciences, subjectName=null, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=8cEfS7jvj/ukyhKVOMJePA==, picEn=cQ12tu98gbNetbFuefeYGg==, jcr=null, cjcr=null, exts=[JournalExt(id=1283836250926859016, language=CN, name=中国临床药理学杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1784020014745, updatedTime=1784020014745, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://lcylauthor.cast.org.cn/webm, submissionEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionReviewUrl=https://lcylauthor.cast.org.cn/webm, submissionCeEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionAeEditorUrl=https://lcyleditor.cast.org.cn/webm, option={"copyright":""}), JournalExt(id=1283836250981384969, language=EN, name=Chinese Journal of Clinical Pharmacology, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1784020014758, updatedTime=1784020014758, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://lcylauthor.cast.org.cn/webm, submissionEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionReviewUrl=https://lcylauthor.cast.org.cn/webm, submissionCeEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionAeEditorUrl=https://lcyleditor.cast.org.cn/webm, option={"copyright":""})], databaseList=null, tenantJournalId=1246415772164075586, websiteList=[Website(id=1246421196657546140, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1246415772164075586, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN, language=CN, createTime=1775099570670, createBy=18614031015, updateTime=1775099747371, updateBy=18614031015, name=中国临床药理学杂志-中文, tplId=1146099689490845704, title=中国临床药理学杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1246422027293316008, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=articleTextType, value=kx, createTime=1775099768708, updateTime=1775099768708, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027268150181, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=banner, value=null, createTime=1775099768702, updateTime=1775099768702, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027310093227, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=grayFlag, value=0, createTime=1775099768712, updateTime=1775099768712, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027259761572, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=logo, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/file/pic?fileId=KeUk4pyVjfIIkOq40uZX0Q==, createTime=1775099768700, updateTime=1775099768700, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027322676141, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=minRunFlag, value=0, createTime=1775099768715, updateTime=1775099768715, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027284927399, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/file/pic, createTime=1775099768706, updateTime=1775099768706, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027318481836, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=silenceFlag, value=0, createTime=1775099768714, updateTime=1775099768714, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027276538790, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1775099768704, updateTime=1775099768704, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027297510313, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=themeColor, value=null, createTime=1775099768709, updateTime=1775099768709, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027305898922, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=themeStyle, value=null, createTime=1775099768711, updateTime=1775099768711, creator=18614031015, updator=18614031015)]), Website(id=1246421196871455649, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1246415772164075586, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN, language=EN, createTime=1775099570720, createBy=18614031015, updateTime=1775099757298, updateBy=18614031015, name=中国临床药理学杂志-英文, tplId=1146101810881728533, title=Chinese Journal of Clinical Pharmacology, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1246422058998059954, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=articleTextType, value=kx, createTime=1775099776267, updateTime=1775099776267, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058977088431, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=banner, value=null, createTime=1775099776262, updateTime=1775099776262, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059014837173, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=grayFlag, value=0, createTime=1775099776271, updateTime=1775099776271, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058968699822, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=logo, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/file/pic?fileId=KeUk4pyVjfIIkOq40uZX0Q==, createTime=1775099776260, updateTime=1775099776260, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059031614391, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=minRunFlag, value=0, createTime=1775099776275, updateTime=1775099776275, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058993865649, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/file/pic, createTime=1775099776266, updateTime=1775099776266, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059023225782, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=silenceFlag, value=0, createTime=1775099776273, updateTime=1775099776273, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058985477040, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1775099776264, updateTime=1775099776264, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059002254259, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=themeColor, value=null, createTime=1775099776268, updateTime=1775099776268, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059010642868, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=themeStyle, value=null, createTime=1775099776270, updateTime=1775099776270, creator=18614031015, updator=18614031015)])], journalTitle=中国临床药理学杂志, weixinUrl=null, journalUrl=null, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Journal of Clinical Pharmacology, journalPhotoCn=8cEfS7jvj/ukyhKVOMJePA==, journalPhotoEn=cQ12tu98gbNetbFuefeYGg==, journalFirstLetter=Z, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.06.016, detailUrlEn=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/10.13699/j.cnki.1001-6821.2025.06.016, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/PDF/10.13699/j.cnki.1001-6821.2025.06.016, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/PDF/10.13699/j.cnki.1001-6821.2025.06.016, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1743091200000, fullTextJson=null, articleText=null, reference=null)
收藏切换
他克莫司对慢性肾小球肾炎大鼠的保护作用
收藏切换
PDF下载
王玮 1 , 王梦婕 1 , 华坚 2
中国临床药理学杂志 | 临床与基础桥接研究 2025,41(6): 834-839
收起
收藏切换
中国临床药理学杂志 |临床与基础桥接研究 2025 , 41 (6) : 834 -839
他克莫司对慢性肾小球肾炎大鼠的保护作用
全屏
[Author(id=1291404676033933808, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291404676281397746, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676033933808, language=EN, stringName=Wei WANG, firstName=Wei, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291404676352700915, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676033933808, language=CN, stringName=王玮, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033, bio={"content":"

王玮（1980-），男，主管药师，主要从事医院药品方面工作及相关研究

"}, bioImg=null, bioContent=

王玮（1980-），男，主管药师，主要从事医院药品方面工作及相关研究

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291404675887133161, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, xref=1., ext=[AuthorCompanyExt(id=1291404675895521770, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China), AuthorCompanyExt(id=1291404675908104683, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033)])]), Author(id=1291404676424004085, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291404676486918647, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676424004085, language=EN, stringName=Meng-jie WANG, firstName=Meng-jie, middleName=null, lastName=WANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291404676646302200, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676424004085, language=CN, stringName=王梦婕, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291404675887133161, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, xref=1., ext=[AuthorCompanyExt(id=1291404675895521770, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China), AuthorCompanyExt(id=1291404675908104683, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675887133161, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033)])]), Author(id=1291404676755354106, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=13698671521@163.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1291404677061538300, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676755354106, language=EN, stringName=Jian HUA, firstName=Jian, middleName=null, lastName=HUA, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.Department of Nephrology, Qingdao Municipal Hospital, Qingdao 266000, Shandong Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291404677128647165, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, authorId=1291404676755354106, language=CN, stringName=华坚, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.青岛市市立医院 肾内科，山东 青岛 266000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291404675962630636, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, xref=2., ext=[AuthorCompanyExt(id=1291404675971019245, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675962630636, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Department of Nephrology, Qingdao Municipal Hospital, Qingdao 266000, Shandong Province, China), AuthorCompanyExt(id=1291404675979407854, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291404566264800036, companyId=1291404675962630636, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.青岛市市立医院 肾内科，山东 青岛 266000)])])]
王玮1, 王梦婕1, 华坚2
作者信息
  • 1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033
  • 2.青岛市市立医院 肾内科，山东 青岛 266000
通讯作者:
华坚，副主任医师 MP: 13698671521 E-mail:
作者简介:

王玮（1980-），男，主管药师，主要从事医院药品方面工作及相关研究

Protective effects of tacrolimus on chronic glomerulonephritis in rats
Wei WANG1, Meng-jie WANG1, Jian HUA2
Affiliations
  • 1.Department of Pharmacy, Qingdao Hiser Hospital Affiliated of Qingdao University/Qingdao Traditional Chinese Medicine Hospital, Qingdao 266033, Shandong Province, China
  • 2.Department of Nephrology, Qingdao Municipal Hospital, Qingdao 266000, Shandong Province, China
出版时间: 2025-03-28 doi: 10.13699/j.cnki.1001-6821.2025.06.016
文章导航
收藏切换
摘要
收起
目的

探究他克莫司通过调控微小RNA-145-5p（miR-145-5p）表达水平介导CXC趋化因子配体16/Ras同系物家族成员A/Rho相关激酶（CXCL16/RhoA/ROCK）通路对慢性肾小球肾炎（CGN）大鼠保护作用的机制。

方法

SD大鼠随机分为对照组（等量0.9% NaCl）、模型组（构建CGN模型+等量0.9% NaCl）、实验组（构建CGN模型+3 mg·kg-1·d-1他克莫司）和联合组（在实验组的基础上经尾静脉注射140 nmol·L-1 miR-145-5p antagomir）。用酶联免疫吸附试验法检测24 h尿蛋白（PRO）、血尿素氮（BUN）、血清肌酸酐（SCr）水平，用实时荧光定量聚合酶链反应法检测大鼠肾组织miR-145-5p的相对表达水平，用蛋白质印迹法检测蛋白相对表达水平。

结果

对照组、模型组、实验组的24 h PRO水平分别为（7.44±2.16）、（85.29±17.35）和（49.81±9.52）mg，BUN水平分别为（5.82±1.03）、（9.65±1.78）和（6.97±1.14）mmol·L-1，SCr水平分别为（46.31±7.69）、（93.57±16.94）和（58.72±8.36）μmol·L-1；对照组、模型组、实验组和联合组的miR-145-5p相对表达水平分别为1.00±0.14、0.56±0.07、0.83±0.12和0.68±0.09，TNF-α水平分别为（116.42±14.53）、（237.56±26.18）、（164.79±18.35）和（205.12±21.64）pg·mL-1，IL-1β水平分别为（15.76±1.84）、（52.41±6.27）、（21.35±2.92）和（43.63±4.58）pg·mL-1，IL-6水平分别为（26.28±3.47）、（71.53±8.64）、（38.92±5.06）和（59.78±6.21）pg·mL-1，CXCL16蛋白相对表达水平分别为1.00±0.12、1.62±0.17、1.23±0.14和1.45±0.15，模型组的上述指标与对照组相比，实验组的上述指标与模型组相比，联合组的上述指标和实验组相比，在统计学上差异均有统计学意义（P<0.05，P<0.01，P<0.001）。

结论

他克莫司可上调miR-145-5p激活CXCL16/RhoA/ROCK通路，减少炎性因子，改善CGN大鼠肾损伤。

他克莫司  /  微小分子核糖核酸145-5p  /  CXC配体16  /  Rho相关蛋白激酶  /  慢性肾小球肾炎
Objective

To explore the mechanism of tacrolimus mediating the protective effect on chronic glomerulonephritis (CGN) rats through regulating the expression of microRNA-145-5p (miR-145-5p) and modulating the C-X-C motif chemokine ligand 16/Ras homolog family member A/Rho-associated coiled-coil-containing protein kinase (CXCL16/RhoA/ROCK) pathway.

Methods

SD rats were randomly divided into the control group (administered an equal volume of 0.9% NaCl), model group (CGN model + equal volume of 0.9% NaCl), experimental group (CGN model + 3 mg·kg-1·d-1 tacrolimus) and combined group (experimental group + tail vein injection of 140 nmol·L-1 miR-145-5p antagomir). Enzyme-linked immunosorbent assay was used to measure 24-hour urinary protein (PRO), blood urea nitrogen (BUN), and serum creatinine (SCr) levels. Real-time quantitative polymerase chain reaction was used to detect the relative expression level of miR-145-5p in rat kidney tissue; Western blotting was used to determine protein expression levels.

Results

The 24-hour PRO levels in the control, model and experimental groups were (7.44±2.16), (85.29±17.35) and (49.81±9.52) mg, respectively; the BUN levels were (5.82±1.03), (9.65±1.78) and (6.97±1.14) mmol·L-1, respectively; the SCr levels were (46.31±7.69), (93.57±16.94) and (58.72±8.36) μmol·L-1, respectively. The relative expression levels of miR-145-5p in the control, model, experimental and combined groups were 1.00±0.14, 0.56±0.07, 0.83±0.12 and 0.68±0.09, respectively; the TNF-α levels were (116.42±14.53), (237.56±26.18), (164.79±18.35) and (205.12±21.64) pg·mL-1, respectively; the IL-1β levels were (15.76±1.84), (52.41±6.27), (21.35±2.92) and (43.63±4.58) pg·mL-1, respectively; the IL-6 levels were (26.28±3.47), (71.53±8.64), (38.92±5.06) and (59.78±6.21) pg·mL-1, respectively; the CXCL16 expression levels were 1.00±0.12, 1.62±0.17, 1.23±0.14 and 1.45±0.15, respectively. Statistical analysis revealed significant differences between the model group and the control group, between the experimental group and the model group, and between the combined group and the experimental group for these indicators (P<0.05, P<0.01, P<0.001).

Conclusion

Tacrolimus can upregulate miR-145-5p, activate the CXCL16/RhoA/ROCK pathway, reduce inflammatory factors, and improve kidney damage in CGN rats.

tacrolimus  /  microRNA-145-5p  /  CXC ligand 16  /  Rho-associated protein kinase  /  chronic glomerulonephritis
王玮, 王梦婕, 华坚. 他克莫司对慢性肾小球肾炎大鼠的保护作用. 中国临床药理学杂志, 2025 , 41 (6) : 834 -839 . DOI: 10.13699/j.cnki.1001-6821.2025.06.016
Wei WANG, Meng-jie WANG, Jian HUA. Protective effects of tacrolimus on chronic glomerulonephritis in rats[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (6) : 834 -839 . DOI: 10.13699/j.cnki.1001-6821.2025.06.016

文献
收起
参考文献 引证文献
排序方式：
2025年第41卷第6期
PDF下载
3
1
引用本文
BibTeX
文章信息
doi: 10.13699/j.cnki.1001-6821.2025.06.016
  • 接收时间：2024-12-04
  • 首发时间：2026-08-04
  • 出版时间：2025-03-28
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2024-12-04
基金
作者信息
    1.青岛大学 附属青岛市海慈医院/青岛市中医医院 药剂科，山东 青岛 266033
    2.青岛市市立医院 肾内科，山东 青岛 266000

通讯作者:

华坚，副主任医师 MP: 13698671521 E-mail:
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.06.016
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257（仅加盟咨询）
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持