中国临床药理学杂志

白头翁皂苷B4通过miR-142-3p对非小细胞肺癌细胞恶性生物学行为的研究

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鲁文君 ^1a , 李小军 ^1b , 张振帅 ^1c

中国临床药理学杂志 | 临床与基础桥接研究 2025,41(6): 784-789

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中国临床药理学杂志 |临床与基础桥接研究 2025 , 41 (6) : 784 -789

白头翁皂苷B4通过miR-142-3p对非小细胞肺癌细胞恶性生物学行为的研究

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鲁文君^1a, 李小军^1b, 张振帅^1c

作者信息

^1a.甘肃省武威肿瘤医院，胸外科，甘肃武威 733000

^1b.甘肃省武威肿瘤医院，肿瘤放疗科，甘肃武威 733000

^1c.甘肃省武威肿瘤医院，急诊科，甘肃武威 733000

通讯作者:

李小军，副主任医师 MP: 13150160200 E-mail: anglwe@sina.com

作者简介:

鲁文君（1979-），男，副主任医师，主要从事食管癌、肺癌方面的临床诊疗工作

Research on the malignant biological behavior of non-small cell lung cancer cells mediated by anemoside B4 via miR-142-3p

Wen-jun LU^1a, Xiao-jun LI^1b, Zhen-shuai ZHANG^1c

Affiliations

^1a.Chest Surgery Department, Gansu Wuwei Tumour Hospital, Wuwei 733000, Gansu Province, China

^1b.Oncology Radiotherapy Department, Gansu Wuwei Tumour Hospital, Wuwei 733000, Gansu Province, China

^1c.Emergency Department, Gansu Wuwei Tumour Hospital, Wuwei 733000, Gansu Province, China

出版时间: 2025-03-28 doi: 10.13699/j.cnki.1001-6821.2025.06.007

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摘要

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目的

研究白头翁皂苷B4通过微小RNA-142-3p（miR-142-3p）介导高迁移率族蛋白A1（HMGA1）表达对非小细胞肺癌（NSCLC）细胞恶性生物学行为的影响机制。

方法

将A549细胞分为A549组（正常培养）、白头翁皂苷B4组（100 μg·mL^-1白头翁皂苷B4处理）、inhibitor NC组（100 μg·mL^-1白头翁皂苷B4+转染inhibitor NC处理）、miR-142-3p inhibitor组（100 μg·mL^-1白头翁皂苷B4+转染miR-142-3p inhibitor处理）、si-NC组（100 μg·mL^-1白头翁皂苷B4+共转染miR-142-3p inhibitor和si-NC处理）、si-HMGA1组（100 μg·mL^-1白头翁皂苷B4+共转染miR-142-3p inhibitor和si-HMGA1处理）。用实时荧光定量逆转录聚合酶链反应和蛋白质印迹法分别检测miR-142-3p、HMGA1的相对表达水平，用5-乙炔基-2′脱氧尿嘧啶核苷（Edu）、Transwell、原位末端标记（TUNEL）法检测细胞的增殖、迁移及凋亡情况。

结果

A549组、白头翁皂苷B4组、inhibitor NC组和miR-142-3p inhibitor组的miR-142-3p相对表达水平分别为1.00±0.14、1.58±0.18、1.60±0.21和1.12±0.16，HMGA1蛋白相对表达水平分别为1.00±0.23、0.56±0.07、0.59±0.08和0.95±0.21，增殖率分别为（84.62±9.11）%、（62.15±7.20）%、（63.89±7.77）%和（76.35±8.11）%，迁移数量分别为（156.23±17.54）、（80.65±8.67）、（82.15±8.96）和（111.55±12.75）个，凋亡率分别为（8.96±0.95）%、（32.56±3.56）%、（33.95±3.79）%和（16.89±1.92）%。si-NC组和si-HMGA1组的增殖率分别为（75.98±8.05）%和（64.52±7.25）%，迁移数量分别为（118.85±12.98）和（86.95±9.21）个，凋亡率分别为（17.58±2.02）%和（28.15±3.13）%。A549组的上述指标与白头翁皂苷B4组比较，miR-142-3p inhibitor组的上述指标与inhibitor NC组比较，si-HMGA1组的上述指标与si-NC组比较，在统计学上差异均有统计学意义（均P<0.05）。

结论

白头翁皂苷B4可能通过调控miR-142-3p/HMGA1轴抑制NSCLC细胞的增殖和迁移，并促进其凋亡。

关键词

白头翁皂苷B4 / 高迁移率组A1 / 非小细胞肺癌 / 细胞凋亡 / 生物学行为

Abstract

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Objective

To investigate the effect of anemoside B4 on the malignant biological behavior of non-small cell lung cancer (NSCLC) cells mediated by microRNA-142-3p (miR-142-3p) on the expression of high mobility group protein A1 (HMGA1).

Methods

A549 cells were divided into A549 group (normal culture), anemoside B4 group (100 μg·mL^-1 anemoside B4 treatment), inhibitor NC group (100 μg·mL^-1 anemoside B4+ transfection inhibitor NC treatment), miR-142-3p inhibitor group (100 μg·mL^-1 anemoside B4+ transfected with miR-142-3p inhibitor treatment), si-NC group (100 μg·mL^-1 anemoside B4+ co-transfected with miR-142-3p inhibitor and si-NC treatment), si-HMGA1 group (100 μg·mL^-1 anemoside B4+ co-transfected with miR-142-3p inhibitor and si-HMGA1 treatment). Real-time fluorescence quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting (WB) were used to detect the expression of miR-142-3p and HMGA1. Cell proliferation, migration and apoptosis were detected by 5-acetylidene-2′-deoxyuracil riboside (Edu), Transwell and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL).

Results

The miR-142-3p relative expression levels of A549 group, anemoside B4 group, inhibitor NC group and miR-142-3p inhibitor group were 1.00±0.14, 1.58±0.18, 1.60±0.21 and 1.12±0.16, respectively; the relative expression levels of HMGA1 protein were 1.00±0.23, 0.56±0.07, 0.59±0.08 and 0.95±0.21, respectively; the proliferation rates were (84.62±9.11)%, (62.15±7.20)%, (63.89±7.77)% and (76.35±8.11)%, respectively; the migration number was (156.23±17.54), (80.65±8.67), (82.15±8.96) and (111.55±12.75) cells, respectively; the apoptosis rates were (8.96±0.95)%, (32.56±3.56)%, (33.95±3.79)% and (16.89±1.92)%, respectively. The proliferation rates of si-NC group and si-HMGA1 group were (75.98±8.05)% and (64.52±7.25)%, respectively; the migration number was (118.85±12.98) and (86.95±9.21) cells, and the apoptosis rates were (17.58±2.02)% and (28.15±3.13)%, respectively. The above indexes in A549 group were compared with anemoside B4 group, miR-142-3p inhibitor group were compared with inhibitor NC group, si-HMGA1 group were compared with si-NC group, the differences were all with statistically significant (all P<0.05).

Conclusion

Anemoside B4 may inhibit proliferation and migration of NSCLC cells and promote apoptosis by regulating the miR-142-3p/HMGA1 axis.

Key words

anemoside B4 / high mobility group protein A1 / non-small cell lung cancer / cell apoptosis / biological behavior

引用本文

鲁文君, 李小军, 张振帅. 白头翁皂苷B4通过miR-142-3p对非小细胞肺癌细胞恶性生物学行为的研究. 中国临床药理学杂志, 2025 , 41 (6) : 784 -789 . DOI: 10.13699/j.cnki.1001-6821.2025.06.007

Wen-jun LU, Xiao-jun LI, Zhen-shuai ZHANG. Research on the malignant biological behavior of non-small cell lung cancer cells mediated by anemoside B4 via miR-142-3p[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (6) : 784 -789 . DOI: 10.13699/j.cnki.1001-6821.2025.06.007

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2025年第41卷第6期

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doi: 10.13699/j.cnki.1001-6821.2025.06.007

接收时间：2024-10-25
首发时间：2026-08-04
出版时间：2025-03-28

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收稿日期：2024-10-25

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作者信息

^1a.甘肃省武威肿瘤医院，胸外科，甘肃武威 733000

^1b.甘肃省武威肿瘤医院，肿瘤放疗科，甘肃武威 733000

^1c.甘肃省武威肿瘤医院，急诊科，甘肃武威 733000

通讯作者:

李小军，副主任医师 MP: 13150160200 E-mail: anglwe@sina.com

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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