Article(id=1291339168316117904, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291339070622396983, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.03.007, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1722268800000, receivedDateStr=2024-07-30, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785808849648, onlineDateStr=2026-08-04, pubDate=1739721600000, pubDateStr=2025-02-17, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785808849648, onlineIssueDateStr=2026-08-04, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785808849648, creator=13041195026, updateTime=1785808849648, updator=13041195026, issue=Issue{id=1291339070622396983, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='3', pageStart='301', pageEnd='450', issueExtLink='null', onlineDate='null', pubDate='1739721600000', pubDateStr='2025-02-17', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785808826353, creator='13041195026', updateTime=1785808826353, updator='13041195026', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=330, endPage=334, ext={EN=ArticleExt(id=1291339168802657169, articleId=1291339168316117904, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Effects of
miRNA-633 regulate BMP / Smad pathway on proliferation and dentition differentiation of dental pulp stem cells, columnId=1246531407326105792, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Clinical and Basic Bridging Research, runingTitle=null, highlight=null, articleAbstract=
Objective To investigate the role of miRNA-633 in targeting the regulation of bone morphogenetic protein (BMP) and Smad protein pathways in promoting the proliferation and differentiation of dental pulp stem cells into dentin.
Methods Human dental pulp stem cells were randomly divided into control, ov-miRNA-633, NC, anti-miRNA-633 and cotransfection groups. The ov-miRNA-633 and control groups were transfected with miRNA-633 overexpression vector and corresponding control blank vector, respectively. The anti-miRNA-633 and NC groups were transfected with miRNA-633 knockout vector and corresponding control blank vector, respectively. The cotransfection group was transfected with miRNA-633 overexpression vector + shRNA-N-Cadherin. Cell proliferation was detected by CCK-8 method. The expression levels of N-Cadherin were detected by real-time polymerase chain reaction. The expression levels of phosphorylated Smad 1/5/8 (p-Smad 1/5/8) were detected by Western blotting.
Results The optical density values on the 7th day of the control, ov-miRNA-633, NC, anti-miRNA-633 and cotransfection groups were 1.65±0.05, 1.79±0.08, 1.55±0.05, 1.19±0.08 and 1.76±0.07; the relative expression levels of N-Cadherin mRNA were 1.09±0.03, 0.63±0.03, 1.06±0.04, 1.31±0.09 and 0.46±0.06; the relative expression levels of p-Smad 1/5/8 protein were 0.79±0.05, 1.28±0.08, 0.80±0.09, 0.62±0.06 and 1.09±0.18, respectively. The differences of above indexes were statistically significant between the ov miRNA-633 group and the control group (P<0.05, P<0.01). And the differences of above indexes were statistically significant between the cotransfection group and the anti miRNA-633 group (all P<0.05).
Conclusion miRNA-633 could promote the proliferation and differentiation of dental pulp stem cells into dentin by inhibiting N-cadherin expression, activating the BMPs/Smad pathway.
, authors=Li-li QIAN
1, Xiao LIU
2, authorsList=Li-li QIAN, Xiao LIU, authorCompany=null, correspAuthors=Xiao LIU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291339169700238226, articleId=1291339168316117904, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=
miRNA-633调控BMP/Smad蛋白通路对牙髓干细胞增殖和成牙本质向分化的影响, columnId=1246531407485489349, journalTitle=中国临床药理学杂志, columnName=临床与基础桥接研究, runingTitle=null, highlight=null, articleAbstract=
目的 研究miRNA-633靶向调控骨形态发生蛋白(BMP)和Smad蛋白通路促进牙髓干细胞增殖和成牙本质向分化的作用。
方法 将人牙髓干细胞随机分为对照组、ov-miRNA-633组、NC组、anti-miRNA-633组和共转染组。Ov-miRNA-633组和对照组分别转染miRNA-633过表达载体和对应对照空白载体,anti-miRNA-633组和NC组分别转染miRNA-633敲除载体和对应对照空白载体,共转染组转染miRNA-633敲除载体+shRNA-N-Cadherin。用CCK-8法检测细胞的增殖情况,用实时荧光定量聚合酶链反应法检测N-黏蛋白(N-Cadherin)的相对表达水平,用蛋白质印迹法检测磷酸化Smad1/5/8(p-Smad 1/5/8)的相对表达水平。
结果 对照组、ov-miRNA-633组、NC组、anti-miRNA-633组和共转染组的第7天增殖活性(光密度值)分别为1.65±0.05、1.79±0.08、1.55±0.05、1.19±0.08和1.76±0.07,N-Cadherin mRNA相对表达水平分别为1.09±0.03、0.63±0.03、1.06±0.04、1.31±0.09和0.46±0.06,p-Smad 1/5/8蛋白相对表达水平分别为0.79±0.05、1.28±0.08、0.80±0.09、0.62±0.06和1.09±0.18。Ov-miRNA-633组的上述指标和对照组比较,共转染组的上述指标和anti-miRNA-633组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。
结论 miRNA-633可以通过抑制N-Cadherin表达,激活BMPs/Smad通路,从而促进牙髓干细胞增殖和成牙本质向分化。
, authors=钱莉莉
1, 刘啸
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钱莉莉(1982-),女,医师,主要从事口腔内科方向的相关研究
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