Article(id=1291339168316117904, tenantId=1146029695717560320, journalId=1246415772164075586, issueId=1291339070622396983, articleNumber=null, orderNo=null, doi=10.13699/j.cnki.1001-6821.2025.03.007, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1722268800000, receivedDateStr=2024-07-30, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1785808849648, onlineDateStr=2026-08-04, pubDate=1739721600000, pubDateStr=2025-02-17, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1785808849648, onlineIssueDateStr=2026-08-04, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1785808849648, creator=13041195026, updateTime=1785808849648, updator=13041195026, issue=Issue{id=1291339070622396983, tenantId=1146029695717560320, journalId=1246415772164075586, year='2025', volume='41', issue='3', pageStart='301', pageEnd='450', issueExtLink='null', onlineDate='null', pubDate='1739721600000', pubDateStr='2025-02-17', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1785808826353, creator='13041195026', updateTime=1785808826353, updator='13041195026', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=330, endPage=334, ext={EN=ArticleExt(id=1291339168802657169, articleId=1291339168316117904, tenantId=1146029695717560320, journalId=1246415772164075586, language=EN, title=Effects of miRNA-633 regulate BMP / Smad pathway on proliferation and dentition differentiation of dental pulp stem cells, columnId=1246531407326105792, journalTitle=Chinese Journal of Clinical Pharmacology, columnName=Clinical and Basic Bridging Research, runingTitle=null, highlight=null, articleAbstract=
Objective

To investigate the role of miRNA-633 in targeting the regulation of bone morphogenetic protein (BMP) and Smad protein pathways in promoting the proliferation and differentiation of dental pulp stem cells into dentin.

Methods

Human dental pulp stem cells were randomly divided into control, ov-miRNA-633, NC, anti-miRNA-633 and cotransfection groups. The ov-miRNA-633 and control groups were transfected with miRNA-633 overexpression vector and corresponding control blank vector, respectively. The anti-miRNA-633 and NC groups were transfected with miRNA-633 knockout vector and corresponding control blank vector, respectively. The cotransfection group was transfected with miRNA-633 overexpression vector + shRNA-N-Cadherin. Cell proliferation was detected by CCK-8 method. The expression levels of N-Cadherin were detected by real-time polymerase chain reaction. The expression levels of phosphorylated Smad 1/5/8 (p-Smad 1/5/8) were detected by Western blotting.

Results

The optical density values on the 7th day of the control, ov-miRNA-633, NC, anti-miRNA-633 and cotransfection groups were 1.65±0.05, 1.79±0.08, 1.55±0.05, 1.19±0.08 and 1.76±0.07; the relative expression levels of N-Cadherin mRNA were 1.09±0.03, 0.63±0.03, 1.06±0.04, 1.31±0.09 and 0.46±0.06; the relative expression levels of p-Smad 1/5/8 protein were 0.79±0.05, 1.28±0.08, 0.80±0.09, 0.62±0.06 and 1.09±0.18, respectively. The differences of above indexes were statistically significant between the ov miRNA-633 group and the control group (P<0.05, P<0.01). And the differences of above indexes were statistically significant between the cotransfection group and the anti miRNA-633 group (all P<0.05).

Conclusion

miRNA-633 could promote the proliferation and differentiation of dental pulp stem cells into dentin by inhibiting N-cadherin expression, activating the BMPs/Smad pathway.

, authors=Li-li QIAN1, Xiao LIU2, authorsList=Li-li QIAN, Xiao LIU, authorCompany=null, correspAuthors=Xiao LIU, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1291339169700238226, articleId=1291339168316117904, tenantId=1146029695717560320, journalId=1246415772164075586, language=CN, title=miRNA-633调控BMP/Smad蛋白通路对牙髓干细胞增殖和成牙本质向分化的影响, columnId=1246531407485489349, journalTitle=中国临床药理学杂志, columnName=临床与基础桥接研究, runingTitle=null, highlight=null, articleAbstract=
目的

研究miRNA-633靶向调控骨形态发生蛋白(BMP)和Smad蛋白通路促进牙髓干细胞增殖和成牙本质向分化的作用。

方法

将人牙髓干细胞随机分为对照组、ov-miRNA-633组、NC组、anti-miRNA-633组和共转染组。Ov-miRNA-633组和对照组分别转染miRNA-633过表达载体和对应对照空白载体,anti-miRNA-633组和NC组分别转染miRNA-633敲除载体和对应对照空白载体,共转染组转染miRNA-633敲除载体+shRNA-N-Cadherin。用CCK-8法检测细胞的增殖情况,用实时荧光定量聚合酶链反应法检测N-黏蛋白(N-Cadherin)的相对表达水平,用蛋白质印迹法检测磷酸化Smad1/5/8(p-Smad 1/5/8)的相对表达水平。

结果

对照组、ov-miRNA-633组、NC组、anti-miRNA-633组和共转染组的第7天增殖活性(光密度值)分别为1.65±0.05、1.79±0.08、1.55±0.05、1.19±0.08和1.76±0.07,N-Cadherin mRNA相对表达水平分别为1.09±0.03、0.63±0.03、1.06±0.04、1.31±0.09和0.46±0.06,p-Smad 1/5/8蛋白相对表达水平分别为0.79±0.05、1.28±0.08、0.80±0.09、0.62±0.06和1.09±0.18。Ov-miRNA-633组的上述指标和对照组比较,共转染组的上述指标和anti-miRNA-633组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。

结论

miRNA-633可以通过抑制N-Cadherin表达,激活BMPs/Smad通路,从而促进牙髓干细胞增殖和成牙本质向分化。

, authors=钱莉莉1, 刘啸2, authorsList=钱莉莉, 刘啸, authorCompany=null, correspAuthors=刘啸, authorNote=

钱莉莉(1982-),女,医师,主要从事口腔内科方向的相关研究

, correspAuthorsNote=
刘啸,副主任医师,硕士生导师 MP: 15955502396 E-mail:
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=dbb3GyRcLbpkTa4gVsalGg==, magXml=ED8dGXGg3kCuEhrf7WA8Hg==, pdfUrl=null, pdf=X4Z2VS1DUi/teFDdF6lmKg==, pdfFileSize=1418068, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=rLbp3YJK+EvP02oVe3CtUQ==, mapNumber=null, fund=null)}, authors=[Author(id=1291339171520566170, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291339171608646556, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, authorId=1291339171520566170, language=EN, stringName=Li-li QIAN, firstName=Li-li, middleName=null, lastName=QIAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.Department of Stomatology, North District, The First People’s Hospital of Chuzhou, Chuzhou 239000, Anhui Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291339171684144029, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, authorId=1291339171520566170, language=CN, stringName=钱莉莉, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.滁州市第一人民医院 北区 口腔科,安徽 滁州 239000, bio={"content":"

钱莉莉(1982-),女,医师,主要从事口腔内科方向的相关研究

"}, bioImg=null, bioContent=

钱莉莉(1982-),女,医师,主要从事口腔内科方向的相关研究

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291339171327628179, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, xref=1., ext=[AuthorCompanyExt(id=1291339171340211092, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171327628179, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Department of Stomatology, North District, The First People’s Hospital of Chuzhou, Chuzhou 239000, Anhui Province, China), AuthorCompanyExt(id=1291339171348599701, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171327628179, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.滁州市第一人民医院 北区 口腔科,安徽 滁州 239000)])]), Author(id=1291339171742864287, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=yanyanyx2018@163.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1291339171835138977, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, authorId=1291339171742864287, language=EN, stringName=Xiao LIU, firstName=Xiao, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.Department of Stomatology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291339171898053538, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, authorId=1291339171742864287, language=CN, stringName=刘啸, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.新疆医科大学 第二附属医院 口腔科,新疆维吾尔自治区 乌鲁木齐 830000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291339171432485782, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, xref=2., ext=[AuthorCompanyExt(id=1291339171436680087, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171432485782, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Department of Stomatology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China), AuthorCompanyExt(id=1291339171445068696, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171432485782, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.新疆医科大学 第二附属医院 口腔科,新疆维吾尔自治区 乌鲁木齐 830000)])])], keywords=[Keyword(id=1291339171986133923, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=EN, orderNo=1, keyword=miRNA-633), Keyword(id=1291339172061631396, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=EN, orderNo=2, keyword=dental pulp stem cell), Keyword(id=1291339172116157349, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=EN, orderNo=3, keyword=N-Cadherin), Keyword(id=1291339172170683302, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=EN, orderNo=4, keyword=differentiation of dentin), Keyword(id=1291339172241986471, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=EN, orderNo=5, keyword=bone morphogenetic protein/Smad protein pathway), Keyword(id=1291339172321678248, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=CN, orderNo=1, keyword=miRNA-633), Keyword(id=1291339172481061801, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=CN, orderNo=2, keyword=牙髓干细胞), Keyword(id=1291339172560753578, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=CN, orderNo=3, keyword=N-钙黏蛋白), Keyword(id=1291339172653028267, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=CN, orderNo=4, keyword=成牙本质向分化), Keyword(id=1291339172715942828, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, language=CN, orderNo=5, keyword=骨形态发生蛋白/Smad蛋白通路)], refs=null, funds=[Fund(id=1291339172833383341, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, awardId=2023D01E31, language=CN, fundingSource=新疆维吾尔自治区杰出青年科学基金资助项目(2023D01E31), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1291339171327628179, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, xref=1., ext=[AuthorCompanyExt(id=1291339171340211092, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171327628179, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Department of Stomatology, North District, The First People’s Hospital of Chuzhou, Chuzhou 239000, Anhui Province, China), AuthorCompanyExt(id=1291339171348599701, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171327628179, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.滁州市第一人民医院 北区 口腔科,安徽 滁州 239000)]), AuthorCompany(id=1291339171432485782, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, xref=2., ext=[AuthorCompanyExt(id=1291339171436680087, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171432485782, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Department of Stomatology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China), AuthorCompanyExt(id=1291339171445068696, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171432485782, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.新疆医科大学 第二附属医院 口腔科,新疆维吾尔自治区 乌鲁木齐 830000)])], figs=null, attaches=null, journal=Journal(id=1246415058658443328, delFlag=0, nameCn=中国临床药理学杂志, nameEn=Chinese Journal of Clinical Pharmacology, nameHistory1=null, nameHistory2=null, issn=1001-6821, eissn=null, cn=11-2220/R, coden=null, periodic=3, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=8cEfS7jvj/ukyhKVOMJePA==, journalPrice=null, startedYear=null, abbrevIsoEn=Chinese Journal of Clinical Pharmacology, journalRemark=null, publicationField=null, createdTime=1775098107259, updatedTime=1784020014723, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=Z, firstLetterEn=Z, subjectCode=Life Sciences, subjectName=null, subjectCodeEn=Life Sciences, subjectNameEn=null, picCn=8cEfS7jvj/ukyhKVOMJePA==, picEn=cQ12tu98gbNetbFuefeYGg==, jcr=null, cjcr=null, exts=[JournalExt(id=1283836250926859016, language=CN, name=中国临床药理学杂志, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1784020014745, updatedTime=1784020014745, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://lcylauthor.cast.org.cn/webm, submissionEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionReviewUrl=https://lcylauthor.cast.org.cn/webm, submissionCeEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionAeEditorUrl=https://lcyleditor.cast.org.cn/webm, option={"copyright":""}), JournalExt(id=1283836250981384969, language=EN, name=Chinese Journal of Clinical Pharmacology, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1784020014758, updatedTime=1784020014758, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://lcylauthor.cast.org.cn/webm, submissionEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionReviewUrl=https://lcylauthor.cast.org.cn/webm, submissionCeEditorUrl=https://lcyleditor.cast.org.cn/webm, submissionAeEditorUrl=https://lcyleditor.cast.org.cn/webm, option={"copyright":""})], databaseList=null, tenantJournalId=1246415772164075586, websiteList=[Website(id=1246421196657546140, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1246415772164075586, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN, language=CN, createTime=1775099570670, createBy=18614031015, updateTime=1775099747371, updateBy=18614031015, name=中国临床药理学杂志-中文, tplId=1146099689490845704, title=中国临床药理学杂志, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1246422027293316008, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=articleTextType, value=kx, createTime=1775099768708, updateTime=1775099768708, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027268150181, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=banner, value=null, createTime=1775099768702, updateTime=1775099768702, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027310093227, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=grayFlag, value=0, createTime=1775099768712, updateTime=1775099768712, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027259761572, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=logo, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/file/pic?fileId=KeUk4pyVjfIIkOq40uZX0Q==, createTime=1775099768700, updateTime=1775099768700, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027322676141, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=minRunFlag, value=0, createTime=1775099768715, updateTime=1775099768715, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027284927399, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/file/pic, createTime=1775099768706, updateTime=1775099768706, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027318481836, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=silenceFlag, value=0, createTime=1775099768714, updateTime=1775099768714, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027276538790, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1775099768704, updateTime=1775099768704, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027297510313, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=themeColor, value=null, createTime=1775099768709, updateTime=1775099768709, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422027305898922, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196657546140, code=themeStyle, value=null, createTime=1775099768711, updateTime=1775099768711, creator=18614031015, updator=18614031015)]), Website(id=1246421196871455649, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1246415772164075586, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN, language=EN, createTime=1775099570720, createBy=18614031015, updateTime=1775099757298, updateBy=18614031015, name=中国临床药理学杂志-英文, tplId=1146101810881728533, title=Chinese Journal of Clinical Pharmacology, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1246422058998059954, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=articleTextType, value=kx, createTime=1775099776267, updateTime=1775099776267, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058977088431, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=banner, value=null, createTime=1775099776262, updateTime=1775099776262, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059014837173, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=grayFlag, value=0, createTime=1775099776271, updateTime=1775099776271, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058968699822, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=logo, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/file/pic?fileId=KeUk4pyVjfIIkOq40uZX0Q==, createTime=1775099776260, updateTime=1775099776260, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059031614391, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=minRunFlag, value=0, createTime=1775099776275, updateTime=1775099776275, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058993865649, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/file/pic, createTime=1775099776266, updateTime=1775099776266, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059023225782, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=silenceFlag, value=0, createTime=1775099776273, updateTime=1775099776273, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422058985477040, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1775099776264, updateTime=1775099776264, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059002254259, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=themeColor, value=null, createTime=1775099776268, updateTime=1775099776268, creator=18614031015, updator=18614031015), WebsiteProps(id=1246422059010642868, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1246421196871455649, code=themeStyle, value=null, createTime=1775099776270, updateTime=1775099776270, creator=18614031015, updator=18614031015)])], journalTitle=中国临床药理学杂志, weixinUrl=null, journalUrl=null, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Journal of Clinical Pharmacology, journalPhotoCn=8cEfS7jvj/ukyhKVOMJePA==, journalPhotoEn=cQ12tu98gbNetbFuefeYGg==, journalFirstLetter=Z, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.03.007, detailUrlEn=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/10.13699/j.cnki.1001-6821.2025.03.007, pdfUrlCn=https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/PDF/10.13699/j.cnki.1001-6821.2025.03.007, pdfUrlEn=https://castjournals.cast.org.cn/joweb/zglcylxzz/EN/PDF/10.13699/j.cnki.1001-6821.2025.03.007, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1739721600000, fullTextJson=null, articleText=null, reference=null)
收藏切换
miRNA-633调控BMP/Smad蛋白通路对牙髓干细胞增殖和成牙本质向分化的影响
收藏切换
PDF下载
钱莉莉 1 , 刘啸 2
中国临床药理学杂志 | 临床与基础桥接研究 2025,41(3): 330-334
收起
收藏切换
中国临床药理学杂志 |临床与基础桥接研究 2025 , 41 (3) : 330 -334
miRNA-633调控BMP/Smad蛋白通路对牙髓干细胞增殖和成牙本质向分化的影响
全屏
[Author(id=1291339171520566170, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1291339171608646556, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, authorId=1291339171520566170, language=EN, stringName=Li-li QIAN, firstName=Li-li, middleName=null, lastName=QIAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.Department of Stomatology, North District, The First People’s Hospital of Chuzhou, Chuzhou 239000, Anhui Province, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291339171684144029, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, authorId=1291339171520566170, language=CN, stringName=钱莉莉, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.滁州市第一人民医院 北区 口腔科,安徽 滁州 239000, bio={"content":"

钱莉莉(1982-),女,医师,主要从事口腔内科方向的相关研究

"}, bioImg=null, bioContent=

钱莉莉(1982-),女,医师,主要从事口腔内科方向的相关研究

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291339171327628179, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, xref=1., ext=[AuthorCompanyExt(id=1291339171340211092, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171327628179, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Department of Stomatology, North District, The First People’s Hospital of Chuzhou, Chuzhou 239000, Anhui Province, China), AuthorCompanyExt(id=1291339171348599701, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171327628179, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.滁州市第一人民医院 北区 口腔科,安徽 滁州 239000)])]), Author(id=1291339171742864287, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=yanyanyx2018@163.com, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1291339171835138977, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, authorId=1291339171742864287, language=EN, stringName=Xiao LIU, firstName=Xiao, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.Department of Stomatology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1291339171898053538, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, authorId=1291339171742864287, language=CN, stringName=刘啸, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2.新疆医科大学 第二附属医院 口腔科,新疆维吾尔自治区 乌鲁木齐 830000, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1291339171432485782, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, xref=2., ext=[AuthorCompanyExt(id=1291339171436680087, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171432485782, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Department of Stomatology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China), AuthorCompanyExt(id=1291339171445068696, tenantId=1146029695717560320, journalId=1246415772164075586, articleId=1291339168316117904, companyId=1291339171432485782, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.新疆医科大学 第二附属医院 口腔科,新疆维吾尔自治区 乌鲁木齐 830000)])])]
钱莉莉1, 刘啸2
作者信息
  • 1.滁州市第一人民医院 北区 口腔科,安徽 滁州 239000
  • 2.新疆医科大学 第二附属医院 口腔科,新疆维吾尔自治区 乌鲁木齐 830000
通讯作者:
刘啸,副主任医师,硕士生导师 MP: 15955502396 E-mail:
作者简介:

钱莉莉(1982-),女,医师,主要从事口腔内科方向的相关研究

Effects of miRNA-633 regulate BMP / Smad pathway on proliferation and dentition differentiation of dental pulp stem cells
Li-li QIAN1, Xiao LIU2
Affiliations
  • 1.Department of Stomatology, North District, The First People’s Hospital of Chuzhou, Chuzhou 239000, Anhui Province, China
  • 2.Department of Stomatology, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
出版时间: 2025-02-17 doi: 10.13699/j.cnki.1001-6821.2025.03.007
文章导航
收藏切换
目的

研究miRNA-633靶向调控骨形态发生蛋白(BMP)和Smad蛋白通路促进牙髓干细胞增殖和成牙本质向分化的作用。

方法

将人牙髓干细胞随机分为对照组、ov-miRNA-633组、NC组、anti-miRNA-633组和共转染组。Ov-miRNA-633组和对照组分别转染miRNA-633过表达载体和对应对照空白载体,anti-miRNA-633组和NC组分别转染miRNA-633敲除载体和对应对照空白载体,共转染组转染miRNA-633敲除载体+shRNA-N-Cadherin。用CCK-8法检测细胞的增殖情况,用实时荧光定量聚合酶链反应法检测N-黏蛋白(N-Cadherin)的相对表达水平,用蛋白质印迹法检测磷酸化Smad1/5/8(p-Smad 1/5/8)的相对表达水平。

结果

对照组、ov-miRNA-633组、NC组、anti-miRNA-633组和共转染组的第7天增殖活性(光密度值)分别为1.65±0.05、1.79±0.08、1.55±0.05、1.19±0.08和1.76±0.07,N-Cadherin mRNA相对表达水平分别为1.09±0.03、0.63±0.03、1.06±0.04、1.31±0.09和0.46±0.06,p-Smad 1/5/8蛋白相对表达水平分别为0.79±0.05、1.28±0.08、0.80±0.09、0.62±0.06和1.09±0.18。Ov-miRNA-633组的上述指标和对照组比较,共转染组的上述指标和anti-miRNA-633组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。

结论

miRNA-633可以通过抑制N-Cadherin表达,激活BMPs/Smad通路,从而促进牙髓干细胞增殖和成牙本质向分化。

miRNA-633  /  牙髓干细胞  /  N-钙黏蛋白  /  成牙本质向分化  /  骨形态发生蛋白/Smad蛋白通路
Objective

To investigate the role of miRNA-633 in targeting the regulation of bone morphogenetic protein (BMP) and Smad protein pathways in promoting the proliferation and differentiation of dental pulp stem cells into dentin.

Methods

Human dental pulp stem cells were randomly divided into control, ov-miRNA-633, NC, anti-miRNA-633 and cotransfection groups. The ov-miRNA-633 and control groups were transfected with miRNA-633 overexpression vector and corresponding control blank vector, respectively. The anti-miRNA-633 and NC groups were transfected with miRNA-633 knockout vector and corresponding control blank vector, respectively. The cotransfection group was transfected with miRNA-633 overexpression vector + shRNA-N-Cadherin. Cell proliferation was detected by CCK-8 method. The expression levels of N-Cadherin were detected by real-time polymerase chain reaction. The expression levels of phosphorylated Smad 1/5/8 (p-Smad 1/5/8) were detected by Western blotting.

Results

The optical density values on the 7th day of the control, ov-miRNA-633, NC, anti-miRNA-633 and cotransfection groups were 1.65±0.05, 1.79±0.08, 1.55±0.05, 1.19±0.08 and 1.76±0.07; the relative expression levels of N-Cadherin mRNA were 1.09±0.03, 0.63±0.03, 1.06±0.04, 1.31±0.09 and 0.46±0.06; the relative expression levels of p-Smad 1/5/8 protein were 0.79±0.05, 1.28±0.08, 0.80±0.09, 0.62±0.06 and 1.09±0.18, respectively. The differences of above indexes were statistically significant between the ov miRNA-633 group and the control group (P<0.05, P<0.01). And the differences of above indexes were statistically significant between the cotransfection group and the anti miRNA-633 group (all P<0.05).

Conclusion

miRNA-633 could promote the proliferation and differentiation of dental pulp stem cells into dentin by inhibiting N-cadherin expression, activating the BMPs/Smad pathway.

miRNA-633  /  dental pulp stem cell  /  N-Cadherin  /  differentiation of dentin  /  bone morphogenetic protein/Smad protein pathway
钱莉莉, 刘啸. miRNA-633调控BMP/Smad蛋白通路对牙髓干细胞增殖和成牙本质向分化的影响. 中国临床药理学杂志, 2025 , 41 (3) : 330 -334 . DOI: 10.13699/j.cnki.1001-6821.2025.03.007
Li-li QIAN, Xiao LIU. Effects of miRNA-633 regulate BMP / Smad pathway on proliferation and dentition differentiation of dental pulp stem cells[J]. Chinese Journal of Clinical Pharmacology, 2025 , 41 (3) : 330 -334 . DOI: 10.13699/j.cnki.1001-6821.2025.03.007
  • 新疆维吾尔自治区杰出青年科学基金资助项目(2023D01E31)
参考文献 引证文献
排序方式:
2025年第41卷第3期
PDF下载
3
1
引用本文
BibTeX
文章信息
doi: 10.13699/j.cnki.1001-6821.2025.03.007
  • 接收时间:2024-07-30
  • 首发时间:2026-08-04
  • 出版时间:2025-02-17
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期:2024-07-30
基金
新疆维吾尔自治区杰出青年科学基金资助项目(2023D01E31)
作者信息
    1.滁州市第一人民医院 北区 口腔科,安徽 滁州 239000
    2.新疆医科大学 第二附属医院 口腔科,新疆维吾尔自治区 乌鲁木齐 830000

通讯作者:

刘啸,副主任医师,硕士生导师 MP: 15955502396 E-mail:
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2025.03.007
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏