中国临床药理学杂志

莪术醇通过降低Siglec-15的表达抑制肝细胞癌增殖迁移的研究

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连海文 ¹ , 关璐璐 ² , 李诚煜 ³ , 郭彩茹 ² , 亓民 ²^,⁴

中国临床药理学杂志 | 临床与基础桥接研究 2026,42(8): 1144-1150

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中国临床药理学杂志 |临床与基础桥接研究 2026 , 42 (8) : 1144 -1150

莪术醇通过降低Siglec-15的表达抑制肝细胞癌增殖迁移的研究

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连海文¹, 关璐璐², 李诚煜³, 郭彩茹², 亓民²^,⁴

作者信息

^1.河南医药大学研究生院，河南新乡 453003

^2.洛阳市中心医院洛阳市肝癌个体化治疗重点实验室，河南洛阳 471000

^3.郑州大学，河南郑州 450000

^4.洛阳市中心医院老年医学科，河南洛阳 471000

通讯作者:

亓民，主任医师，硕士生导师 MP: 15037922015 E-mail: Qimin19720629@163.com

作者简介:

连海文（2002-），女，硕士研究生，主要从事消化系统疾病方面的研究

Research of curcumol inhibiting the proliferation and migration of hepatocellular carcinoma by downregulating Siglec-15 expression

Hai-wen LIAN¹, Lu-lu GUAN², Cheng-yu LI³, Cai-ru GUO², Min QI²^,⁴

Affiliations

^1.Graduate School of Henan Medical University, Xinxiang 453003, Henan Province, China

^2.Luoyang Key Laboratory of Individualized Treatment of Liver Cancer, Luoyang Central Hospital, Luoyang 471000, Henan Province, China

^3.Zhengzhou University, Zhengzhou 450000, Henan Province, China

^4.Department of Geriatrics, Luoyang Central Hospital, Luoyang 471000, Henan Province, China

出版时间: 2026-04-28 doi: 10.13699/j.cnki.1001-6821.2026.08.015

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摘要

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目的

研究莪术醇（curcumol）通过唾液酸结合免疫球蛋白样凝集素15（Siglec-15）对于抑制肝细胞癌（HCC）生长的效果和机制。

方法

随机将Bel-7402和HepG2细胞分别分为对照组（正常培养）、低剂量实验组（50 μmol·L^-1莪术醇干预48 h）和高剂量实验组（100 μmol·L^-1莪术醇干预48 h）。用噻唑蓝（MTT）比色法、Transwell小室实验法、划痕愈合实验法及流式细胞术，分别评估细胞的增殖、侵袭及迁移能力，通过实时荧光定量聚合酶链反应（RT-qPCR）法和蛋白质免疫印迹法对Siglec-15、磷脂酰肌醇3-激酶（PI3K）、蛋白激酶B（AKT）信号通路相关分子的信使RNA（mRNA）与蛋白相对表达水平进行检测。

结果

Bel-7402细胞对照组、低、高剂量实验组与HepG2细胞的对照组、低、高剂量实验组中的细胞存活率分别为（100.00±0）%、（72.09±1.07）%、（55.65±2.99）%与（100.00±0）%、（57.38±6.97）%、（44.63±6.04）%，Bel-7402细胞与HepG2细胞的各组凋亡率分别为（1.87±0.05）%、（2.77±0.08）%、（4.19±0.11）%与（3.52±0.17）%、（5.46±0.11）%、（6.63±0.16）%，Bel-7402细胞与HepG2细胞的各组划痕愈合率分别为（39.06±0.81）%、（19.20±0.59）%、（8.34±0.89）%与（52.25±1.43）%、（21.10±1.95）%、（15.31±0.80）%，Bel-7402细胞与HepG2细胞的纵向迁移率分别为（100.00±0）%、（72.40±4.34）%、（62.47±3.08）%与（100.00±0）%、（60.51±2.62）%、（40.47±2.76）%，在Bel-7402细胞的对照组及低、高剂量实验组与HepG2细胞的对照组及低、高剂量实验组中，Siglec-15 mRNA的相对表达水平测定分别为1.00±0.18、0.89±0.01、0.69±0.07与1.00±0.09、0.90±0.04、0.88±0.03，PI3K mRNA相对表达水平分别为1.00±0.16、0.80±0.12、0.75±0.11与1.00±0.16、0.93±0.08、0.93±0.04，AKT mRNA相对表达水平分别为1.00±0.15、0.73±0.17、0.52±0.11与1.00±0.21、0.69±0.06、0.27±0.05，Siglec-15蛋白相对表达水平分别为1.05±0.07、0.91±0.09、0.84±0.07与0.99±0.05、0.80±0.02、0.64±0.04，PI3K蛋白相对表达水平分别为0.96±0.09、0.49±0.10、0.17±0.10与1.01±0.03、0.73±0.03、0.49±0.03，AKT蛋白相对表达水平分别为0.98±0.04、0.88±0.08、0.63±0.04与1.06±0.04、0.92±0.02、0.67±0.03。2种细胞系中，与对照组相比，各实验组的上述指标，在统计学上差异均具有统计学意义（均P<0.001）。

结论

莪术醇可通过下调Siglec-15表达，抑制肝癌细胞Bel7402、HepG2的增殖与迁移，发挥抗肝细胞癌作用。

关键词

莪术醇 / 肝癌 / 唾液酸结合免疫球蛋白样凝集素15 / 磷脂酰肌醇3-激酶/蛋白激酶B信号通路 / 增殖 / 迁移

Abstract

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Objective

To investigate the inhibitory effect of curcumol on the growth of hepatocellular carcinoma(HCC)cells through sialic acid-binding immunoglobulin-like lectin 15(Siglec-15)and its underlying mechanism.

Methods

Bel-7402 and HepG2 cells were randomly divided into control group(normal culture), low-dose experimental group (treated with 50 μmol·L^-1 curcumol for 48 hours), and a high-dose experimental group (treated with 100 μmol·L^-1 curcumol for 48 hours). The proliferative, invasive, and migratory capacities of cells were evaluated through a combination of functional assays, including the methyl thiazolyl tetrazolium (MTT) method for cell viability, Transwell and wound healing assays for migration and invasion, and flow cytometry for additional cellular characteristics. The expression levels of Siglec-15, along with key molecules involved in the phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT) signaling cascade, were examined at both the messenger RNA(mRNA) and protein levels. For this purpose, real-time quantitative polymerase chain reaction (RT-qPCR) was employed to assess transcript abundance, while Western blotting was used to determine protein expression.

Results

The cell viability rates in the control, low-dose, and high-dose experimental groups of Bel-7402 cells and HepG2 cells were (100.00±0)%, (72.09±1.07)%, (55.65±2.99)% and (100.00±0)%, (57.38±6.97)%, (44.63±6.04)%, respectively; the apoptosis rates in the corresponding groups of Bel-7402 cells and HepG2 cells were (1.87±0.05)%, (2.77±0.08)%, (4.19±0.11)% and (3.52±0.17)%, (5.46±0.11)%, (6.63±0.16)%, respectively; the wound healing rates (horizontal migration rates) in the corresponding groups of Bel-7402 cells and HepG2 cells were (39.06±0.81)%, (19.20±0.59)%, (8.34±0.89)% and (52.25±1.43)%, (21.10±1.95)%, (15.31±0.80)%, respectively; the vertical migration rates in the corresponding groups of Bel-7402 cells and HepG2 cells were (100.00±0)%, (72.40±4.34)%, (62.47±3.08)% and (100.00±0)%, (60.51±2.62)%, (40.47±2.76)%, respectively. In the control group, low-dose experimental group, and high-dose experimental group of Bel-7402 cells and HepG2 cells, the relative mRNA expression levels of Siglec-15 were 1.00±0.18, 0.89±0.01, 0.69±0.07 and 1.00±0.09, 0.90±0.04, 0.88±0.03, respectively; the relative mRNA expression levels of PI3K were 1.00±0.16, 0.80±0.12, 0.75±0.11 and 1.00±0.16, 0.93±0.08, 0.93±0.04, respectively; the relative mRNA expression levels of AKT were 1.00±0.15, 0.73±0.17, 0.52±0.11 and 1.00±0.21, 0.69±0.06, 0.27±0.05, respectively; the relative protein expression levels of Siglec-15 were 1.05±0.07, 0.91±0.09,0.84±0.07 and 0.99±0.05, 0.80±0.02, 0.64±0.04, respectively; the relative protein expression levels of PI3K were 0.96±0.09, 0.49±0.10, 0.17±0.10 and 1.01±0.03, 0.73±0.03, 0.49±0.03, respectively. The relative protein expression levels of AKT were 0.98±0.04, 0.88±0.08, 0.63±0.04 and 1.06±0.04, 0.92±0.02, 0.67±0.03, respectively. In both cell lines, compared with the control group, the differences in the above indicators between each experimental group and the control group were all statistically significant (all P<0.001).

Conclusion

Curcumol exerts anti-hepatocellular carcinoma effects by downregulating Siglec-15 expression and inhibiting the proliferation and migration of Bel-7402 and HepG2 hepatoma cells.

Key words

curcumol / hepatocellular carcinoma / sialic acid-binding immunoglobulin-like lectin 15 / phosphatidylinositol 3-kinase/protein kinase B pathway / proliferation / migration

引用本文

连海文, 关璐璐, 李诚煜, 郭彩茹, 亓民. 莪术醇通过降低Siglec-15的表达抑制肝细胞癌增殖迁移的研究. 中国临床药理学杂志, 2026 , 42 (8) : 1144 -1150 . DOI: 10.13699/j.cnki.1001-6821.2026.08.015

Hai-wen LIAN, Lu-lu GUAN, Cheng-yu LI, Cai-ru GUO, Min QI. Research of curcumol inhibiting the proliferation and migration of hepatocellular carcinoma by downregulating Siglec-15 expression[J]. Chinese Journal of Clinical Pharmacology, 2026 , 42 (8) : 1144 -1150 . DOI: 10.13699/j.cnki.1001-6821.2026.08.015

基金

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河南省医学科技攻关计划基金资助项目(LHGJ20250771)
河南省科技攻关基金资助项目(252102310123)

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2026年第42卷第8期

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文章信息

doi: 10.13699/j.cnki.1001-6821.2026.08.015

接收时间：2026-01-27
首发时间：2026-08-06
出版时间：2026-04-28

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收稿日期：2026-01-27

基金

河南省医学科技攻关计划基金资助项目(LHGJ20250771)

河南省科技攻关基金资助项目(252102310123)

作者信息

^1.河南医药大学研究生院，河南新乡 453003

^2.洛阳市中心医院洛阳市肝癌个体化治疗重点实验室，河南洛阳 471000

^3.郑州大学，河南郑州 450000

^4.洛阳市中心医院老年医学科，河南洛阳 471000

通讯作者:

亓民，主任医师，硕士生导师 MP: 15037922015 E-mail: Qimin19720629@163.com

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https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2026.08.015

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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