中国临床药理学杂志

汉黄芩素通过LncRNA SNHG16/miR-302a-3p/E2F1分子轴调控胃癌细胞生物学行为研究

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常毅刚 , 霍华英

中国临床药理学杂志 | 临床与基础桥接研究 2026,42(7): 954-961

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中国临床药理学杂志 |临床与基础桥接研究 2026 , 42 (7) : 954 -961

汉黄芩素通过LncRNA SNHG16/miR-302a-3p/E2F1分子轴调控胃癌细胞生物学行为研究

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常毅刚, 霍华英

作者信息

山西省人民医院胃肠胰科，山西太原 030012

通讯作者:

霍华英，主任医师，硕士生导师 MP: 13753130258 E-mail: gracechang82@163.com

作者简介:

常毅刚（1976-），男，主任医师，主要从事胃癌、结直肠癌、胰腺癌及后腹膜肿瘤方面的临床工作和研究

Research of baicalin regulates the biological behavior of gastric cancer cells through the LncRNA SNHG16/miR-302a-3p/E2F1 molecular axis

Yi-gang CHANG, Hua-ying HUO

Affiliations

Department of Gastrointestinal and Pancreatic Surgery, Shanxi Provincial People's Hospital, Taiyuan 030012, Shanxi Province, China

出版时间: 2026-04-17 doi: 10.13699/j.cnki.1001-6821.2026.07.009

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摘要

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目的

探讨汉黄芩素（WOG）通过长链非编码核糖核酸小核仁核糖核酸宿主基因16（LncRNA SNHG16）/微小核糖核酸（miR）-302a-3p/E2F转录因子1（E2F1）对胃癌细胞生物学行为的影响。

方法

在细胞实验部分，将人胃癌细胞（MGC-803）分为细胞对照组（正常培养）、细胞高剂量实验组（100 μmol·L^-1 WOG处理细胞）、oe-NC组（转染oe-NC）、oe-LncSNHG16组（转染oe-LncSNHG16）、mimic-NC组（转染mimic-NC）、miR-302a-3p mimic组（转染miR-302a-3p mimic）、汉黄芩素+oe-NC组（在100 μmol·L^-1 WOG处理组基础上转染oe-NC）、汉黄芩素+oe-SNHG16组（在100 μmol·L^-1 WOG处理组基础上转染oe-SNHG16）和汉黄芩素+oe-LncSNHG16+miR-302a-3p mimic组（在100 μmol·L^-1WOG处理组基础上转染oe-LncSNHG16和miR-302a-3p mimic）。在动物试验部分，在Balb/c雄性裸鼠中建立胃癌皮下移植瘤模型，将建模成功的裸鼠随机分为动物低、中、高剂量试验组（15、30、60 mg·kg^-1 WOG灌胃处理），动物对照组裸鼠灌胃等体积生理盐水，每组10只。用实时荧光定量聚合酶链式反应（qRT-PCR）法检测LncRNA SNHG16、miR-302a-3p和E2F1相对表达水平，用细胞计数试剂盒8（CCK-8）法检测细胞存活率，用5-乙炔基-2’脱氧尿嘧啶核苷（Edu）法、Transwell法及原位末端转移酶标记技术（TUNEL）法分别检测细胞增殖、侵袭及凋亡情况。

结果

在细胞实验部分，细胞对照组、细胞高剂量实验组、mimic-NC组、miR-302a-3p mimic组、oe-NC组和oe-LncSNHG16组的LncRNA SNHG16相对表达水平分别为1.00±0.13、0.47±0.05、1.00±0.10、0.81±0.12、1.02±0.09和3.76±0.41，miR-302a-3p相对表达水平分别为1.00±0.11、3.60±0.39、1.00±0.09、3.74±0.32、0.97±0.10和0.27±0.03，E2F1 mRNA相对表达水平分别为1.00±0.14、0.23±0.02、1.00±0.10、0.33±0.03、0.95±0.08和2.18±0.25，细胞高浓度实验组与细胞对照组相比、miR-302a-3p mimic组与mimic-NC组对比、oe-LncSNHG16组与oe-NC组相比，上述指标在统计学上差异均有统计学意义（P<0.01，P<0.001）。细胞对照组、细胞高剂量实验组、汉黄芩素+oe-LncSNHG16组和汉黄芩素+oe-LncSNHG16+miR-302a-3p mimic组的细胞增殖率分别为（95.04±10.14）%、（36.36±4.75）%、（97.64±11.04）%和（31.87±3.56）%，侵袭细胞个数分别为（147.36±15.01）、（43.31±6.25）、（153.45±15.22）和（37.35±5.04）个，细胞凋亡率分别为（7.83±0.83）%、（35.77±4.38）%、（8.44±0.88）%和（29.35±3.09）%，细胞高剂量实验组的上述指标与细胞对照组比较、汉黄芩素+oe-LncSNHG16组的上述指标与实验组比较、汉黄芩素+oe-LncSNHG16组的上述指标与汉黄芩素+oe-LncSNHG16+miR-302a-3p mimic组比较，在统计学上差异均有统计学意义（均P<0.001）。在动物试验部分，动物对照组和动物低、中、高剂量试验组的肿瘤体积分别为（2 554.50±280.41）、（2 194.10±234.22）、（1 512.40±166.75）和（1 146.20±124.32）mm³，肿瘤质量分别为（2.13±0.28）、（1.84±0.23）、（1.65±0.21）和（1.32±0.14）g，动物低、中、高剂量实验组的上述指标与动物对照组比较，在统计学上差异均有统计学意义（P<0.05，P<0.01，P<0.001）。

结论

WOG可以抑制胃癌细胞的增殖和侵袭，并促进其凋亡，这可能与调控LncRNA SNHG16/miR-302a-3p/E2F1轴阻滞细胞周期有关。

关键词

汉黄芩素 / 长链非编码核糖核酸小核仁核糖核酸宿主基因16 / E2F转录因子1 / 胃癌 / 细胞周期 / 增殖 / 侵袭 / 凋亡

Abstract

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Objective

To explore the effects of woginostatin (WOG) on the biological behaviors of gastric cancer cells through long non-coding RNA small nucleolar RNA host gene 16 (LncRNA SNHG16)/microRNA (miR)-302a-3p/E2F transcription factor 1 (E2F1).

Methods

In cell experiment, human gastric cancer cells (MGC-803) were divided into cell control group (normal culture), cell experimental-H group (100 μmol·L^-1 WOG treated cells), oe-NC group (transfected oe-NC), oe-LncSNHG16 group (transfected oe-LncSNHG16), mimic-NC group (transfected mimic-NC), miR-302a-3p mimic group (transfected miR-302a-3p mimic), WOG + oe-NC group (transfected oe-NC based on 100 μmol·L^-1 WOG treated cells), WOG + oe-LncSNHG16 group (transfected oe-LncSNHG16 based on 100 μmol·L^-1 WOG treated cells), WOG +oe-LncSNHG16+miR-302a-3p mimic group (transfection of oe-LncSNHG16 and miR-302a-3p mimic on the basis of 100 μmol·L^-1WOG treatment group). In animal experiment, the subcutaneous transplanted tumor model of gastric cancer was established in BALB/C male nude mice. The nude mice were randomly divided into animal experimental-L, -M and -H dose groups (15, 30 and 60 mg·kg^-1 WOG gavage), and the animal control group was gavaged with equal volume of physiological saline, with 10 mice in each group. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the relative expression levels of LncRNA SNHG16, miR-302a-3p and E2F1. The cell viability was detected using the cell counting kit 8 (CCK-8). The cell proliferation, invasion and apoptosis were detected using 5-ethynyl-2′-deoxyuridine (Edu), Transwell and in situ terminal transferase labeling technique (TUNEL).

Results

In cell experiment, the relative expression levels of LncRNASNHG16 in cell control group, cell experimental-H group, mimic-NC group, miR-302a-3p mimic group, oe-NC group and oe- LncSNHG16 group were 1.00±0.13, 0.47±0.05, 1.00±0.10, 0.81±0.12, 1.02±0.09 and 3.76±0.41, respectively; the relative expression levels of miR-302a-3p were 1.00±0.11, 3.60±0.39, 1.00±0.09, 3.74±0.32, 0.97±0.10 and 0.27±0.03, respectively; the relative expression levels of E2F1 mRNA were 1.00±0.14, 0.23±0.02, 1.00±0.10, 0.33±0.03, 0.95±0.08 and 2.18±0.25, respectively. Compared the cell experimental-H group with cell control group, compared miR-302a-3p mimic group with mimic-NC group and compared oe-LncSNHG16 group with oe-NC group, the above indicators were all statistically significantly different (P<0.01, P<0.001). The proliferation rates of cell control group, cell experimental-H group, WOG + oe-LncSNHG16 group and WOG + oe-LncSNHG16 + miR-302a-3p mimic group were (95.04±10.14)%, (36.36±4.75)%, (97.64±11.04)% and (31.87±3.56)%, respectively; the number of invasive cells were (147.36±15.01), (43.31±6.25), (153.45±15.22) and (37.35±5.04), respectively; the apoptosis rates were (7.83±0.83)%, (35.77±4.38)%, (8.44±0.88)% and (29.35±3.09)%, respectively. There were statistically significant differences in the above indexes between the cell experimental-H group and the cell control group, between the WOG + oe-LncSNHG16 group and the cell experimental-H group, and between the WOG + oe-LncSNHG16 group and the WOG + oe-LncSNHG16 + miR-302a-3p mimic group (all P<0.001). In animal experiment, the tumor volume of animal control group and animal experimental-L,-M,-H groups were (2 554.50±280.41), (2 194.10±234.22), (1 512.40±166.75) and (1 146.20±124.32) mm³, respectively; the tumor mass was (2.13±0.28), (1.84±0.23), (1.65±0.21) and (1.32±0.14) g, respectively. There were statistically significant differences in the above indicators between experimental-L, -M, -H groups and animal control group (P<0.05, P<0.01, P<0.001).

Conclusion

WOG can inhibit the proliferation and invasion of gastric cancer cells and promote their apoptosis. This may be related to the regulation of the LncRNA SNHG16/miR-302a-3p/E2F1 axis, which blocks the cell cycle.

Key words

woginostatin / long-chain non-coding ribonucleic acid small nucleolar ribonucleic acid host gene 16 / E2F transcription factor 1 / gastric cancer / cell cycle / proliferation / invasion / apoptosis

引用本文

常毅刚, 霍华英. 汉黄芩素通过LncRNA SNHG16/miR-302a-3p/E2F1分子轴调控胃癌细胞生物学行为研究. 中国临床药理学杂志, 2026 , 42 (7) : 954 -961 . DOI: 10.13699/j.cnki.1001-6821.2026.07.009

Yi-gang CHANG, Hua-ying HUO. Research of baicalin regulates the biological behavior of gastric cancer cells through the LncRNA SNHG16/miR-302a-3p/E2F1 molecular axis[J]. Chinese Journal of Clinical Pharmacology, 2026 , 42 (7) : 954 -961 . DOI: 10.13699/j.cnki.1001-6821.2026.07.009

基金

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山西省中医药管理局科研课题计划基金资助项目(2024ZYYC040)

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2026年第42卷第7期

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文章信息

doi: 10.13699/j.cnki.1001-6821.2026.07.009

接收时间：2026-03-17
首发时间：2026-08-06
出版时间：2026-04-17

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收稿日期：2026-03-17

基金

山西省中医药管理局科研课题计划基金资助项目(2024ZYYC040)

作者信息

山西省人民医院胃肠胰科，山西太原 030012

通讯作者:

霍华英，主任医师，硕士生导师 MP: 13753130258 E-mail: gracechang82@163.com

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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