中国临床药理学杂志

槲皮素调控JAK2-STAT3信号通路对压力性尿失禁模型大鼠盆底功能恢复的影响及作用机制

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陈嫣 ¹^,² , 杨晨 ¹^,² , 叶利群 ¹^,²

中国临床药理学杂志 | 临床与基础桥接研究 2026,42(2): 211-218

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中国临床药理学杂志 |临床与基础桥接研究 2026 , 42 (2) : 211 -218

槲皮素调控JAK2-STAT3信号通路对压力性尿失禁模型大鼠盆底功能恢复的影响及作用机制

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陈嫣¹^,², 杨晨¹^,², 叶利群¹^,²

作者信息

^1.宁波市中医院&浙江中医药大学附属宁波市中医院妇二科，浙江，宁波 315010

^2.浙江中医药大学宁波临床医学院，浙江，宁波 315010

通讯作者:

叶利群，主任中医师，硕导 MP: 13609905058 E-mail: eix292@163.com

作者简介:

陈嫣（1983-）女，副主任中医师，主要从事中医妇科相关研究

The effect and mechanism of quercetin on the recovery of pelvic floor function in a rat model of stress urinary incontinence based on the JAK2-STAT3 signaling pathway

Yan CHEN¹^,², Chen YANG¹^,², Li-qun YE¹^,²

Affiliations

^1.Department of Second Gynecology, Ningbo Hospital of Traditional Chinese Medicine & Ningbo Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese Medical University, Ningbo 315010, Zhejiang Province, China

^2.College of Ningbo Clinical Medical, Zhejiang Chinese Medical University, Ningbo 315010, Zhejiang Province, China

出版时间: 2026-01-28 doi: 10.13699/j.cnki.1001-6821.2026.02.010

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摘要

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目的

探究槲皮素调控Janus激酶2（JAK2）-信号转导子和转录激活子3（STAT3）通路对压力性尿失禁（SUI）模型大鼠盆底功能恢复的影响及机制。

方法

选取SD雌性大鼠，按照每组12只的标准分为对照（NC）组、SUI组、低、中、高剂量槲皮素（L、M、H-槲皮素）组、H-槲皮素+JAK2-STAT3激活剂RO8191（H-槲皮素+RO8191）组，各给药组连续5周每日根据剂量给药。之后检测大鼠尿动力学、膀胱内压、盆底功能相关指标及盆底肌肉表面电信号；ELISA及DCFH-DA荧光探针检测氧化应激指标；HE染色观察尿道组织病理变化；Western blot检测蛋白表达。

结果

SUI组大鼠尿道组织细胞及细胞核密度降低，胞体萎缩且排列失序，着色不均，肌束间间隙增宽、肌层变薄，可见细胞内空泡形成；SUI组较NC组漏尿点压力、最大容积、腹压漏尿点压力、排尿时间间隔、排尿效率、阴道收缩压、阴道静息压、Ⅰ类、Ⅱ类盆底肌纤维电位、超氧化物歧化酶（SOD）、谷胱苷肽过氧化物酶（GSH-Px）降低，残余尿量、排尿量、膀胱颈活动度、尿道旋转角度、丙二醛（MDA）、活性氧（ROS）荧光强度、p-JAK2/JAK2、p-STAT3/STAT3升高（P<0.05）；L、M、H-槲皮素组较SUI组漏尿点压力、最大容积、腹压漏尿点压力、排尿时间间隔、排尿效率、阴道收缩压、阴道静息压、Ⅰ类、Ⅱ类盆底肌纤维电位、SOD、GSH-Px升高，残余尿量、排尿量、膀胱颈活动度、尿道旋转角度、MDA、ROS荧光强度、p-JAK2/JAK2、p-STAT3/STAT3降低（P<0.05）；H-槲皮素+RO8191组较H-槲皮素组漏尿点压力、最大容积、腹压漏尿点压力、排尿时间间隔、排尿效率、阴道收缩压、阴道静息压、Ⅰ类、Ⅱ类盆底肌纤维电位、SOD、GSH-Px降低，残余尿量、排尿量、膀胱颈活动度、尿道旋转角度、MDA、ROS荧光强度、p-JAK2/JAK2、p-STAT3/STAT3升高（P<0.05）。

结论

槲皮素可能通过抑制JAK2-STAT3通路，恢复SUI大鼠盆底功能。

关键词

槲皮素 / JAK2-STAT3通路 / 压力性尿失禁 / 盆底功能

Abstract

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Objective

To investigate the effect and mechanism of quercetin on the recovery of pelvic floor function in a rat model of stress urinary incontinence (SUI) by regulating the Janus kinase 2 (JAK2)-signal transducer and activator of transcription 3 (STAT3) pathway.

Methods

Female SD rats were selected and assigned into the control (NC) group, SUI group, low-, medium-, and high-dose quercetin (L, M, H-quercetin) groups, and H-quercetin+JAK2-STAT3 activator RO8191 (H-quercetin+RO8191) group according to the criteria of 12 rats in each group. Each administration group was administered daily according to the dose, for 5 consecutive weeks. Subsequently, the urodynamics, intravesical pressure, pelvic floor function-related parameters of rats and the surface electrical signals of pelvic floor muscles were detected. The oxidative stress indexes were detected by ELISA and DCFH-DA fluorescent probe. HE staining was used to observe the pathological changes of urethral tissue. Moreover, protein expression was detected by Western blot.

Results

In the SUI group, the density of cells and cell nuclei in the rat urethral tissue decreased, the cells atrophied and were arranged in a disordered manner with uneven staining, the muscular layer became thinner, the interstitial spaces widened, and intracellular vacuolation was observed. The urinary leakage point pressure, maximum volume, abdominal pressure, urinary leakage point pressure when abdominal pressing, urination time interval, urination efficiency, vaginal systolic pressure, vaginal resting pressure, type Ⅰ and type Ⅱ pelvic floor muscle fiber potentials, supeioxide dismucase (SOD) and glutathione peroxidase (GSH-Px) in the SUI group were lower than those in the NC group, while the residual urine volume, urine output, bladder neck range of motion, urethral rotation angle, malondialdhyde(MDA), reactive oxygen species (ROS) fluorescence intensities, p-JAK2/JAK2, and p-STAT3/STAT3 were higher (P<0.05). The urinary leakage point pressure, maximum volume, abdominal pressure, urinary leakage point pressure when abdominal pressing, urination time interval, urination efficiency, vaginal systolic pressure, vaginal resting pressure, type Ⅰ and type Ⅱ pelvic floor muscle fiber potentials, SOD and GSH-Px in the L, M, and H-quercetin groups were higher than those in the SUI group, while the residual urine volume, urine output, bladder neck range of motion, urethral rotation angle, MDA, ROS fluorescence intensities, p-JAK2/JAK2, and p-STAT3/STAT3 were lower (P<0.05). The urinary leakage point pressure, maximum volume, abdominal pressure, urinary leakage point pressure when abdominal pressing, urination time interval, urination efficiency, vaginal systolic pressure, vaginal resting pressure, type Ⅰ and type Ⅱ pelvic floor muscle fiber potentials, SOD and GSH-Px in the H-quercetin+RO8191 group were lower than those in the H-quercetin group, while the residual urine volume, urine output, bladder neck range of motion, urethral rotation angle, MDA, ROS fluorescence intensities, p-JAK2/JAK2, and p-STAT3/STAT3 were higher (P<0.05).

Conclusion

Quercetin may restore pelvic floor function in SUI rats by inhibiting the JAK2-STAT3 pathway.

Key words

quercetin / JAK2-STAT3 pathway / stress urinary incontinence / pelvic floor function

引用本文

陈嫣, 杨晨, 叶利群. 槲皮素调控JAK2-STAT3信号通路对压力性尿失禁模型大鼠盆底功能恢复的影响及作用机制. 中国临床药理学杂志, 2026 , 42 (2) : 211 -218 . DOI: 10.13699/j.cnki.1001-6821.2026.02.010

Yan CHEN, Chen YANG, Li-qun YE. The effect and mechanism of quercetin on the recovery of pelvic floor function in a rat model of stress urinary incontinence based on the JAK2-STAT3 signaling pathway[J]. Chinese Journal of Clinical Pharmacology, 2026 , 42 (2) : 211 -218 . DOI: 10.13699/j.cnki.1001-6821.2026.02.010

基金

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国家中医药管理局科技司-浙江省中医药管理局共建项目(GZY-ZJ-KJ-Z3090)
宁波市公益类科技计划项目(20211JCGY020397)

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2026年第42卷第2期

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doi: 10.13699/j.cnki.1001-6821.2026.02.010

接收时间：2025-12-14
首发时间：2026-08-06
出版时间：2026-01-28

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收稿日期：2025-12-14

基金

国家中医药管理局科技司-浙江省中医药管理局共建项目(GZY-ZJ-KJ-Z3090)

宁波市公益类科技计划项目(20211JCGY020397)

作者信息

^1.宁波市中医院&浙江中医药大学附属宁波市中医院妇二科，浙江，宁波 315010

^2.浙江中医药大学宁波临床医学院，浙江，宁波 315010

通讯作者:

叶利群，主任中医师，硕导 MP: 13609905058 E-mail: eix292@163.com

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https://castjournals.cast.org.cn/joweb/zglcylxzz/CN/10.13699/j.cnki.1001-6821.2026.02.010

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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